Monocytes trafficking into primary tumors and their metastases were studied by adoptive transfer of mouse (Ly6C/Gr1+ or Ly6C/Gr1−) or human (CD14+CD16+ and CD16−) monocytes using MMTV-PyMT autochthonous, human and mouse experimental metastasis and human orthotopic tumor models. Monocytes and macrophages were recovered by enzymatic disaggregation of the tumors followed by FACS analysis. To test mechanisms behind monocyte recruitment and the effect of inhibition of this trafficking on metastasis anti-mouse or human neutralizing CCL2 antibodies or Ccr2 null mutant mice were used. In order to ablate VEGF expression in monocytes a myeloid specific (Csf1r promoter) tamoxifen inducible Cre expressing strain was crossed with VEGFflox/flox mice and gene ablation induced by tamoxifen. Effects of monocyte depletion on tumor cell extravasation using Met-1, a FVB PyMT tumor derived metastatic cell line, was determined using an ex vivo intact lung imaging system and an in vitro extravasation assay.
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Adoptive Transfer
Adoptive Transfer
Adoptive Transfer: The transfer of cells, such as T cells or natural killer cells, from one individual to another to enhance the recipient's immune response against diseases like cancer or infections.
This technique can help optimize research by locating relevant protocols, leveraging AI-comparisons to identify the best approaches, and improving reproducibility and accuracy in your adoptive transfer experiments.
Discover how PubCompare.ai, the leading AI-driven platform, can support your adoptive transfer research and acheive your research goals.
This technique can help optimize research by locating relevant protocols, leveraging AI-comparisons to identify the best approaches, and improving reproducibility and accuracy in your adoptive transfer experiments.
Discover how PubCompare.ai, the leading AI-driven platform, can support your adoptive transfer research and acheive your research goals.
Most cited protocols related to «Adoptive Transfer»
Adoptive Transfer
Antibodies, Neutralizing
Biological Assay
CCL2 protein, human
Cell Line, Tumor
Cells
Enzymes
Gene, c-fms
Genes
Homo sapiens
Lung
Macrophage
Mice, Knockout
Monocytes
Mouse mammary tumor virus
Mus
Neoplasm Metastasis
Neoplasms
Psychological Inhibition
Strains
Tamoxifen
Vascular Endothelial Growth Factors
Adoptive Transfer
Antibodies, Neutralizing
Biological Assay
CCL2 protein, human
Cell Line, Tumor
Cells
Enzymes
Gene, c-fms
Genes
Homo sapiens
Lung
Macrophage
Mice, Knockout
Monocytes
Mouse mammary tumor virus
Mus
Neoplasm Metastasis
Neoplasms
Psychological Inhibition
Strains
Tamoxifen
Vascular Endothelial Growth Factors
Purified nuocytes were cultured for 2 - 5 days in RPMI supplemented with 10 ng/ml IL-33 and 10 ng/ml IL-7. Cells were washed and injected intravenously in PBS via the tail vein at 0.5 × 106 cells/mouse. Adoptive transfer was performed four hours after infection with N. brasiliensis, or 12 hours before the first cytokine administration of IL-25 i.p.
Adoptive Transfer
Cells
Cytokine
IL25 protein, human
IL33 protein, human
Infection
Interleukin-10
Mus
Tail
Veins
Adoptive Transfer
Antibodies
BLOOD
CD8-Positive T-Lymphocytes
cesium chloride
Cloning Vectors
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Gene Expression
HIV Envelope Protein gp120
Homo sapiens
Immunoglobulins
Mus
Muscle, Gastrocnemius
Serum
Transfection
Transients
Ultracentrifugation
Virus
AAV2/8 was produced by transient transfection and purification from culture supernatant by PEG precipitation and cesium chloride ultracentrifugation. Virus was quantified by qPCR against CMV sequences and functionally validated in vitro to confirm gene expression prior to use in vivo. Mice were given single injections with purified vector in the gastrocnemius muscle. Antibody concentration in the serum was determined using an ELISA specific for either total human IgG or human IgG against HIV-gp120. Humanized mice expressing antibodies were produced by adoptive transfer of expanded huPBMCs into mice previously transduced with AAV vectors. HIV challenge was carried out via IP or IV injection and blood was sampled weekly to determine the ratio of CD4 to CD8 cells by flow cytometry.
Adoptive Transfer
Antibodies
BLOOD
CD8-Positive T-Lymphocytes
cesium chloride
Cloning Vectors
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Gene Expression
HIV Envelope Protein gp120
Homo sapiens
Immunoglobulins
Mus
Muscle, Gastrocnemius
Serum
Transfection
Transients
Ultracentrifugation
Virus
Most recents protocols related to «Adoptive Transfer»
HEK293T cells were transfected and treated as described above. On day 2, splenocytes were prepared from STG mice and cultured in 10-cm plates at a density of 5–10 × 107 cells in 15 ml T cell media with soluble LCMV peptide GP61-80 (1 μg/ml; AnaSpec). The following day, activated STG+ T cells were isolated using an EasySep Mouse CD4+ T cell isolation kit (Stemcell Technologies) and plated in 24-well plates at a density of 1–2 × 106 cells per well. Cells were transduced with viral supernatants as described above. Following transduction, cells were washed and resuspended in PBS for adoptive transfer via retro-orbital injection into recipient mice.
Adoptive Transfer
CD4 Positive T Lymphocytes
Cells
isolation
Lymphocytic choriomeningitis virus
Mus
Peptides
Peptide T
Stem Cells
T-Lymphocyte
Adoptive transfers were performed by intravenous injection of 5 × 103 Igλ-enriched B1-8 B cells into the retro-orbital plexus of anesthetized mice. Mice were immunized the following day. All immunizations were performed using NP-CGG (Biosearch Technologies) resuspended at 1 mg/ml in D-PBS and mixed 50:50 volumetrically with Alhydrogel (Accurate Chemical and Scientific). Mice were injected subcutaneously with 20 μl of this solution (10 μg of NP-CGG per injection) in each ear. At endpoint, the facial LNs from each side were pooled for analysis (see figure legends for various timepoints).
Unmodified αIgE (clone R1E4; produced by hybridoma culture as described below), αIgE with a mutated Fc-receptor binding domain (clone R1E4; Cedarlane), or control rat γ globulin were diluted in D-PBS to a concentration of 0.3 mg/ml and injected intravenously to achieve a final dose of 3.25 mg/kg. For the experiment shown, mouse γ globulin (Jackson ImmunoResearch) was used as a control whereas in previous experiments that the presented data are representative of rat γ globulin was used as a control.
Tamoxifen was dissolved at 50 mg/ml in corn oil (Sigma-Aldrich) by shaking at 56°C for several hours. Approximately 100 μl/mouse was delivered by intraperitoneal injection to achieve a dose of 200 mg/kg.
Unmodified αIgE (clone R1E4; produced by hybridoma culture as described below), αIgE with a mutated Fc-receptor binding domain (clone R1E4; Cedarlane), or control rat γ globulin were diluted in D-PBS to a concentration of 0.3 mg/ml and injected intravenously to achieve a final dose of 3.25 mg/kg. For the experiment shown, mouse γ globulin (Jackson ImmunoResearch) was used as a control whereas in previous experiments that the presented data are representative of rat γ globulin was used as a control.
Tamoxifen was dissolved at 50 mg/ml in corn oil (Sigma-Aldrich) by shaking at 56°C for several hours. Approximately 100 μl/mouse was delivered by intraperitoneal injection to achieve a dose of 200 mg/kg.
Adoptive Transfer
Alhydrogel
B-Lymphocytes
Clone Cells
Corn oil
Face
Fc Receptor
gamma-Globulin
Hybridomas
Immunization
Injections, Intraperitoneal
Mice, House
NP 10
Tamoxifen
Single cell suspensions were isolated from spleens and erythrocytes were lysed with lysis buffer. CD4+CD25+ Treg cell isolation kits (Miltenyi) were used to isolate CD4+CD25- cells and perform adoptive transfer colitis.
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Adoptive Transfer
Buffers
CD4 Positive T Lymphocytes
Cells
Cell Separation
Colitis
Erythrocytes
IL2RA protein, human
Tumor cell suspensions, BMDMs, RAW 264.7and TAMs were washed, blocked with Fc Block (anti-mouse CD16/32, BD Biosciences) at 4 °C for 15 min, and stained with fluorescence-conjugated antibodies against surface markers CD4 (clone OKT4), CD8 (clone 17D8), F4/80 (clone BM8), CD11b (clone VIM12), CD86 (clone BU63), CD206 (clone MR5D3) and PD-L1 (clone MIH5) antibodies purchased from BioLegend or BD Biosciences. Cells were then fixed in a fixation/permeabilization buffer (BD Biosciences, 554715) and stained with antibodies against intracellular proteins, including Granzyme B (clone GB11, BioLegend). The apoptosis of cells were detected using Annexin V-FITC/PI Apoptosis Detection Kit (BD Biosciences, 556547). Single cell suspensions were prepared and analyzed using the FACS Calibur (BD Biosciences). FACS-sorted CD206+ macrophages are from BMDMs for adoptive transfer experiments by using a BD FACS Aria-II SORP flow cytometer (BD Biosciences). Data analysis was performed using FlowJo Version7.6 (BD Biosciences).
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Adoptive Transfer
Antibodies
Apoptosis
Buffers
CD274 protein, human
Cells
Clone Cells
FITC-annexin A5
Fluorescent Antibody Technique
GZMB protein, human
ITGAM protein, human
Macrophage
Mus
Myeloproliferative Syndrome, Transient
Neoplasms
NRG1 protein, human
Proteins
Protoplasm
Two passive transfer experiments were conducted using 3–4-week-old mixed-sex A129 mice. For the passive transfer of immune mouse serum, mice (n = 5 per group) were injected with pooled serum from weeks 3, 4, and 5 of the A129 mouse vaccination experiment by IP injection (Supplementary Fig. 1b ). For the passive transfer of macaque serum, mice (n = 6 per group) were injected with pooled serum from week –2 (naive serum) or individual serum from week 8 (immune serum) of the macaque vaccination experiment by IP injection (Supplementary Fig. 1e ).
To determine if vaccination induced protection via cellular immunity, an adoptive transfer experiment was conducted using three groups of 3–4-week-old mixed-sex mice (6 per group). Ten million splenocytes were administered via retro-orbital injection in a volume of 50 µL. Serum samples were collected before challenge or at week 2 after the adoptive transfer (Supplementary Fig.1c ).
Mice were challenged by IP injection of YFV Asibi strain with a dose of 1 × 105 PFU in 50 µL of PBS. The Asibi strain in combination with a higher dose and the IP inoculation route was used to enhance the disease outcome in young A129 mice after wtYFV infection. These changes were prompted by the mild disease outcome observed in adult A129 mice after challenge with a low dose of YFV BeH 622205. Serum viral load was measured on day 3 or 6 after the challenge (Supplementary Fig.1 ). Animals were monitored daily for weight change or signs of disease throughout the course of 2 weeks.
To determine if vaccination induced protection via cellular immunity, an adoptive transfer experiment was conducted using three groups of 3–4-week-old mixed-sex mice (6 per group). Ten million splenocytes were administered via retro-orbital injection in a volume of 50 µL. Serum samples were collected before challenge or at week 2 after the adoptive transfer (Supplementary Fig.
Mice were challenged by IP injection of YFV Asibi strain with a dose of 1 × 105 PFU in 50 µL of PBS. The Asibi strain in combination with a higher dose and the IP inoculation route was used to enhance the disease outcome in young A129 mice after wtYFV infection. These changes were prompted by the mild disease outcome observed in adult A129 mice after challenge with a low dose of YFV BeH 622205. Serum viral load was measured on day 3 or 6 after the challenge (Supplementary Fig.
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Adoptive Transfer
Adult
Animals
Cytoprotection
Immune Sera
Infection
Injections, Intraperitoneal
Macaca
Mice, House
Response, Immune
Serum
Strains
Vaccination
Top products related to «Adoptive Transfer»
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CFSE (Carboxyfluorescein succinimidyl ester) is a fluorescent dye used for cell proliferation and tracking assays. It binds to cellular proteins, allowing the labeling and monitoring of cell division in various cell types.
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The FACSAria is a flow cytometry instrument manufactured by BD. It is used for the analysis and sorting of cells and other particles. The FACSAria is designed to provide high-performance cell sorting capabilities, enabling researchers to isolate specific cell populations for further analysis or experimentation.
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The FACSAria II is a high-performance cell sorter produced by BD. It is designed for precision cell sorting and analysis. The system utilizes flow cytometry technology to rapidly identify and separate different cell populations within a sample.
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CellTrace Violet is a cell tracking dye used for flow cytometry applications to monitor cell division. It binds to intracellular proteins, allowing the dye to be evenly distributed between daughter cells during cell division, enabling the visualization and quantification of cell proliferation.
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C57BL/6J is a mouse strain commonly used in biomedical research. It is a common inbred mouse strain that has been extensively characterized.
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The CD8+ T cell isolation kit from Miltenyi Biotec is designed to isolate CD8+ T cells from a variety of cell samples. The kit utilizes a magnetic bead-based separation technology to selectively enrich for the CD8+ T cell population.
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C57BL/6 is a widely used inbred mouse strain. It is a robust, readily available laboratory mouse model.
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The CD4+ T cell isolation kit is a laboratory equipment product designed for the isolation and enrichment of CD4+ T cells from biological samples. The kit utilizes magnetic separation technology to effectively separate the CD4+ T cell population from other cell types present in the sample.
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Magnetic beads are small, spherical particles that are coated with a magnetic material. They can be used for a variety of laboratory applications, such as cell separation, protein purification, and nucleic acid isolation.
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C57BL/6 mice are a widely used inbred mouse strain commonly used in biomedical research. They are known for their black coat color and are a popular model organism due to their well-characterized genetic and physiological traits.
More about "Adoptive Transfer"
Adoptive Transfer, also known as Cell Transfer or Adoptive Immunotherapy, is a powerful technique used in biomedical research and clinical applications to enhance the immune response against diseases like cancer and infections.
This process involves the transfer of immune cells, such as T cells or natural killer (NK) cells, from one individual to another to bolster the recipient's immune defenses.
In adoptive transfer experiments, researchers often utilize various tools and techniques to isolate, expand, and characterize the transferred cells.
Common methods include the use of CFSE (Carboxyfluorescein Succinimidyl Ester) for cell proliferation tracking, FACSAria or FACSAria II flow cytometers for cell sorting and analysis, and CellTrace Violet for cell division monitoring.
The transfer of cells is often conducted in animal models, such as C57BL/6J mice, to evaluate the efficacy and safety of the approach.
To further optimize adoptive transfer research, scientists may employ cell isolation kits, such as the CD8+ T cell isolation kit or CD4+ T cell isolation kit, to precisely select the desired cell populations.
Magnetic beads are another commonly used tool for cell separation and enrichment.
The C57BL/6 mouse strain is a widely used model in adoptive transfer studies due to its well-characterized immune system and its suitability for various experimental protocols.
PubCompare.ai, the leading AI-driven platform, can support your adoptive transfer research by helping you locate relevant protocols from the literature, preprints, and patents, and by leveraging AI-comparisons to identify the best approaches.
This can improve the reproducibility and accuracy of your adoptive transfer experiments, ultimately accelerating your research goals and discoveries.
This process involves the transfer of immune cells, such as T cells or natural killer (NK) cells, from one individual to another to bolster the recipient's immune defenses.
In adoptive transfer experiments, researchers often utilize various tools and techniques to isolate, expand, and characterize the transferred cells.
Common methods include the use of CFSE (Carboxyfluorescein Succinimidyl Ester) for cell proliferation tracking, FACSAria or FACSAria II flow cytometers for cell sorting and analysis, and CellTrace Violet for cell division monitoring.
The transfer of cells is often conducted in animal models, such as C57BL/6J mice, to evaluate the efficacy and safety of the approach.
To further optimize adoptive transfer research, scientists may employ cell isolation kits, such as the CD8+ T cell isolation kit or CD4+ T cell isolation kit, to precisely select the desired cell populations.
Magnetic beads are another commonly used tool for cell separation and enrichment.
The C57BL/6 mouse strain is a widely used model in adoptive transfer studies due to its well-characterized immune system and its suitability for various experimental protocols.
PubCompare.ai, the leading AI-driven platform, can support your adoptive transfer research by helping you locate relevant protocols from the literature, preprints, and patents, and by leveraging AI-comparisons to identify the best approaches.
This can improve the reproducibility and accuracy of your adoptive transfer experiments, ultimately accelerating your research goals and discoveries.