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Antisepsis

Antisepsis refers to the prevention or reduction of microbial contamination and infection, particularly in medical and surgical settings.
This process involves the use of antiseptic agents, procedures, and techniques to maintain a sterile or aseptic environment.
Effective antisepsis is crucial in healthcare to minimize the risk of hospital-acquired infections and promote patient safety.
Key aspects of antisepsis include hand hygiene, skin preparation, disinfection of medical equipment, and sterile technique during invasive procedures.
Reserchers can enhance their antisepsis studies by utilizing PubCompare.ai, an AI-driven platform that helps identify optimal protocols from academic literature, preprints, and patents.
This tool enables efficient comparison of antisepsis methods and products, boosting research productivity and succes in the field.

Most cited protocols related to «Antisepsis»

The organ culture procedures were modified from published reports (Zheng and Gao, 1996 (link), Ding et al., 2002 (link)). In brief, mice of postnatal day 3 (p3) were euthanized after antisepsis with 70% ethanol, and the inner ears were removed. The cochleae were immersed in cold Hank’s Balanced Salt Solution and the lateral wall tissues (stria vascularis and spiral ligament) and the auditory nerve bundle were dissected away leaving the organ of Corti and spiral ganglion neurons. The explants were placed onto a previously prepared culture dish: a 15-μL drop of rat tail collagen solution was allowed to polymerize for approximately 15 min on a 35 mm dish, then 1 mL of serum-free medium, including Basal Medium Eagle (BME), 1% serum-free supplement (Gibco catalogue number 51500-056; Invitrogen, Carlsbad, CA), 1% bovine serum albumin, 5 mg/mL glucose and 10 U/mL penicillin G were added. The cochlear explants were incubated (37°C, 5% CO2) for 4 h, then an additional 1 mL of culture medium was added to submerse the explants.
Publication 2009
Antisepsis Cochlea Cochlear Nerve Collagen Common Cold Culture Media Dietary Supplements Eagle Ethanol Ganglion of Corti Glucose Hyperostosis, Diffuse Idiopathic Skeletal Labyrinth Mice, House Neurons Organ Culture Techniques Organ of Corti Penicillin G Serum Serum Albumin, Bovine Sodium Chloride Spiral Ligament of Cochlea Stria Vascularis Tail Tissues

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Publication 2009
alexa fluor 488 Animals Antisepsis Axilla Choleragenoid Pneumothorax Respiratory Rate Rib Cage Skin Sterility, Reproductive Syringes Thoracic Cavity
This is a prospective randomized single-blind clinical trial comparing in-plane ulnar approach carpal tunnel injection versus out-plane carpal tunnel injection and blind injection of 40 mg of triamcinolone in patients with idiopathic CTS in terms of efficacy and safety. The patients were maintained in the supine position, with the forearm supinated and the wrist placed in slight dorsiflexion position to prevent change in the appearance of the median nerve according to wrist position (Figure 1).
In blind injection, after skin antisepsis, the 26-gauge needle was inserted into the proximal carpal tunnel at the distal wrist crease just ulnar to the palmaris longus tendon. The out-plane approach was performed using a perpendicularly placed transducer, and the needle was inserted into the proximal carpal tunnel at the distal wrist crease just ulnar to the palmaris longus tendon.4 The needle tip was identified as a moving reflector in real time as the tip passed obliquely from the skin surface to the proximal to distal carpal tunnel. In the in-plane ulnar approach, the transducer is moved ulnarly while keeping the median nerve in view (Figure 2A). In this manner, the pisiform, ulnar nerve, and ulnar artery are brought into view (Figure 2B).4 The pisiform appears as a prominent superiorly rounded hyperechoic structure on the ulnar side of the screen. The ulnar nerve lies just radial to the pisiform, and the ulnar artery lies radial to the ulnar nerve. Doppler images can confirm the position of the ulnar artery.7 (link) Although the optimal injectable location has not been determined, target sign which is produced by injectate as a ring form structure is used as a proper injection guideline.
A typical injectate consists of 1 mL of 40 mg/mL triamcinolone and 1 mL of 1% lidocaine, delivered in equal portions above the nerve, below the nerve, and into the subsynovial connective tissue. After completion of the injection, the distal carpal tunnel is scanned to ensure distribution of injectate throughout the proximal-to-distal extent of the carpal tunnel.7 (link) All injections were performed by the same physician. The US-guided injections were performed using an US device (GE healthcare, Hertfordshire, UK).
Publication 2014
Antisepsis Arteries, Ulnar Carpal Bones Connective Tissue Forearm Lidocaine Medical Devices Needles Nerves, Median Nervousness Patients Physicians Pisiform Bones Radial Nerve Safety Skin Tendons Transducers Triamcinolone Triquetral Bone Ulnar Nerve Visually Impaired Persons Wrist Joint
Animals were anesthetized with a combination of ketamine and xylazine (10:7,
100 mg/kg injected intraperitoneally). The coccygeal intervertebral spaces
Co6-7, Co7-8, and Co8-9 were selected for the study. The needle puncture was
performed at coccygeal intervertebral levels Co6-7 and Co8-9. The intervertebral
level Co7-8 remained undisturbed as the control level. The selected coccygeal
intervertebral levels (Co6-7 and Co8-9) were identified by digital palpation and
confirmed by fluoroscopy. After tail skin antisepsis with alcohol iodate and
using fluoroscopy, a 20-gauge needle was inserted at the level of the annulus
fibrosus of Co6-7 (proximal) and Co8-9 (distal), crossing the nucleus pulposus
up to the contralateral annulus fibrosus. After full penetration, the needle was
rotated 360° twice and held for 30 s. The depth of needle penetration was
controlled by the resistance of the contralateral annulus fibrosus.
Publication 2013
Animals Annular Nucleus Antisepsis Anulus Fibrosus ARID1A protein, human Coccyx Ethanol Fingers Fluoroscopy Iodates Ketamine Needles Palpation Punctures Skin Tail Xylazine
After detailed study of the anorectal morphology on three Wistar and three BDIX rats, we designed a simple procedure, as follows (Figure 1).
Firstly, the animal was placed over a heating blanket, in a supine position. Perineal antisepsis was performed. The anal canal was emptied and a probe (Abbocath® 14G or similar) was inserted through it.
An arciform 10 mm to 15 mm anterior perianal incision (3 mm from verge) was performed. A 6/0 stitch at the inferior border to the tail was knotted. Adipose tissue was dissected sideways (with scissors or gauze) to expose approximately 20 mm of anorectal conduit until visualization of a thin darker line (external sphincter) or up to the anal verge.
Then, the muscular layer was held with clamps and a small incision was made until herniation of the submucosa or the probe was visualized by transparency. Submucosal dissection was continued longitudinally and the muscular layer was sectioned in a longitudinal fashion up to the anal verge and near the stitch caudally and proximally until completing 10 mm. If a mucosal perforation occurred, an interrupted suture was performed, leaving knots on the parietal side.
Publication 2018
Anal Canal Animals Antisepsis Anus ARID1A protein, human Bladder Detrusor Muscle Darkness Dissection Hernia Mucous Membrane Perineum Rattus norvegicus Sutures Tail Tissue, Adipose

Most recents protocols related to «Antisepsis»

The control group will receive the usual treatment following the recommendations for the treatment of skin ulcers of the Madrid Health Service [1 ], which consists of assessment, cleansing, antisepsis, debridement, topical treatment (healing in a moist environment through the use of dressings) and multilayer compression therapy.
The intervention group will receive the usual treatment together with the “Active Legs” intervention, which consists of an evidence-based, structured, nurse-led educational intervention in health centre consultations. It incorporates a home-based lower limb exercise program and daily ambulation guidelines. Figure 1 shows schematically the different components of both the control and the Active Legs interventions. The home lower limb exercise program consists of four exercises of progressive difficulty, which the patient will perform twice a day, at least 5 days a week. In addition, patients will be required to follow a program of progressive daily ambulation until the target of 150 min/week (30 minutes for 5 days a week) is reached [25 ]. The detailed intervention is described using TIDieR (see Additional file 2).

Components of Active Legs complex intervention vs standard clinical practice

Patients will be provided with an “Active Legs” diary containing all the information on the exercise program and a pedometer to record daily steps.
Those who have not reached complete healing at the end of the study will continue with the same treatment until complete healing is achieved and may resume the daily ambulation and exercise program.
In order to avoid possible differences in the application of the Active Legs program, training has been planned beforehand for the nursing professionals participating in the study, including both training in injury assessment and educational intervention.
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Publication 2023
Administration, Topical Antisepsis Debridement Dressings Health Services Administration Injuries Leg Lower Extremity Nurses Patients Skin Ulcer Teaching
Except for the control and CLP groups, silymarin (SIGMA S0292-10G) was given to the other groups in 50 mg/kg, 100 mg/kg, and 200 mg/kg doses with a gavage needle and orally. Silymarin serum was applied after being suspended in physiological water. An hour later, CLP was applied to the third, fourth, and fifth groups (Groups 3, 4, and 5). Oral-applied silymarin reaches the highest level in plasma within approximately 2–4 hr and it has a half-life of 6 hr [72 (link)]. The application of anesthetic material for the operation was given an hour before the silymarin operation because it slowed down the absorption of the drug. Only the CLP model was applied to the second group.
CLP is a method used to create septicemia in polymicrobial properties. The animals in the operation groups were anesthetized with xylazine (5 mg/kg) and ketamine hydrochloride (40 mg/kg). The rats were secured on the back, and the infiltration line of the abdomen was wiped twice with povidone-iodine solution, providing the antisepsis. The midline of the abdomen was opened with a 3 cm incision and the cecum was explored. The ligation was applied under the ileocecal valve with silk floss (3/0). The connected section was drilled twice with the standard injector tip measuring 18 gauge and tightened to allow some of the fecal matter to come out. Then, the cecum was placed in the abdomen and the abdomen was closed with the stapler in normal anatomical form.
In the CLP rat model, the perforation was constituted with 13, 16, 18, and 22 caliber injector tips and a 0.5 cm scalpel, and the mortality of the experimental subjects was assessed in the literature [45 (link)]. Therefore, we used an 18-gauge injector to obtain blood and liver tissue in this study before the rats died.
The operated animals were taken into postoperative care for 16 hr. At the end of 16 hr, 15 mg/kg xylazine and 100 mg/kg ketamine were administered intraperitoneally to all subjects. Thus, the reflexes of the anesthetized rats were controlled and an incision was made at the level of the abdominal diaphragm. The animals whose blood samples were taken from their hearts under deep anesthesia were sacrificed by cervical dislocation. Then, the liver tissues of all animals were taken to a solution containing 10% formaldehyde for histopathological and immunohistochemical studies. The blood samples taken were used for biochemical analysis.
Publication 2023
Abdomen Abdominal Cavity Anesthesia Anesthetics Animals Antisepsis BLOOD Cecum Feces Formaldehyde Heart Ileocecal Valve Joint Dislocations Ketamine Ketamine Hydrochloride Ligation Liver Liver Function Tests Neck Needles Pharmaceutical Preparations physiology Plasma Postoperative Care Povidone Iodine Rattus norvegicus Reflex Septicemia Serum Silk Silymarin Tissues Tube Feeding Vaginal Diaphragm Xylazine
All interventions were performed by two experienced vitreoretinal surgeons (T.C., A.B.) following local anesthesia (peribulbar block) and antisepsis with povidone–iodine solution. Both groups received a standard 25-Gauge three-port vitrectomy (Constellation® Vision System, Alcon Laboratories Inc, Fort Worth, TX, USA) followed by a staining of the epiretinal tissues by the injection of a combination 0.15% TrypanBlue, 0.025% Brilliant Blue G and 4% polyethylene glycol vital dye (Membraneblue-Dual®, DORC International, Zuidland, The Netherlands). Prior to epiretinal membrane peeling, a small bubble of approximately 1.0 cc of Perfluoro-N-Octane (EFTIAR Octane®, DORC International, Zuidland, The Netherlands) was injected over the macula in the PFCL-assisted group.
During the intervention, upon creating a stable flap of epiretinal tissue with end-gripping forceps, the surgeon continued applying a mild traction and proceeded to acquire an iOCT B-Scan (Rescan 700®, Carl Zeiss Meditec AG, Jena, Germany) oriented in parallel to the projection of the vector of traction over the retina (Figure 1a). The acquired scans had a width of 6 mm and an A-Scan depth of 5.8 mm in tissue. The same imaging procedure was repeated whenever the flap was dragged into a different macular subfield or a new flap was started (Figure 2). Superior, nasal, inferior and temporal macular subfields were estimated following a simplified version of the ETDRS grid (Figure 1b). The careful evaluation of intraoperative complications such as the occurrence of retinal tears was conducted. Moreover, an independent observer (G.G.) collected intraoperative data regarding the number of grabs the surgeon had to perform with the forceps in order to complete the ERM removal.
Following a complete peeling of the ERM, the surgeon further proceeded to completely remove any residual ILM, with additional injections of vital dye as needed. In the PFCL-assisted group, the PFCL was then thoroughly removed by active aspiration and fluid/air exchange was performed in both groups.
All OCT images for each eye were exported to external software (ImageJ, NIH, USA; version 1.53e) for postoperative analysis (Figure 1c). Two masked independent ophthalmologists (S.M.P., G.G.) assessed the DA value for individual OCT scans (Figure 3) and then reported the mean value expressed in degrees for each eye for statistical analysis. A minimum of four scans—at least one for each macular subfield—per eye were examined. The interclass correlation coefficient (ICC) was determined to assess interobserver agreement. The statistical analysis of the results was conducted via the IBM SPSS Statistics® software (version 25).
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Publication 2023
Antisepsis brilliant blue G Cloning Vectors Epiretinal Membrane Forceps Intraoperative Complications Local Anesthesia Macula Lutea Nose octane Ophthalmologists perfluorooctane Polyethylene Glycols Povidone Iodine Radionuclide Imaging Retina Retinal Perforations Surgeons Surgical Flaps Tissues Traction Vitrectomy
The animals were subjected to an anesthetic procedure with 2% xylazine hydrochloride (Xilazin®, Syntec, Santana de Parnaíba, Brazil) (10 mg·Kg−1) associated with 5% dextrocetamine hydrochloride (Ketamin®, Cristália, Itapira, Brazil) (25 mg·Kg−1), prepared by combining 0.5 mL of xylazine (10 mg) with 0.5 mL of ketamine (25 mg) to a volume of 1.0 mL, which was administered intraperitoneally (1.0 mL·Kg−1).
After being anesthetized, the respective animals were positioned on appropriate tables in the horizontal prone position, and skin antisepsis with chlorhexidine 2% (RIOHEX®, Rioqumica, So José do Rio Preto, Brazil), followed by 0.5% alcoholic chlorhexidine (RIOHEX®, Rioqumica, So José do Rio Preto, Brazil), was performed as part of the pre-surgical preparation [132 (link)]. With the help of a scalpel (handle and blade number 15), Mezenbaum scissors curve, and anatomical forceps, each animal—having already been identified by its group—had one circular excision of the skin made in the median plane of the dorsal region, which was constrained in depth by the muscular aponeurosis. This was accomplished by precisely measuring the 2 cm diameter of each excision using a caliper and a plastic circular mold (Universal Digimess 100003) [132 (link)]. Thereafter, animals were housed individually and monitored in properly disinfected cages to prevent infection or further damage to the wounds after recovering from anesthesia.
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Publication 2023
Alcoholics Anesthesia Anesthetics Animals Antisepsis Aponeurosis Chlorhexidine Forceps Fungus, Filamentous Infection Ketamine Muscle Tissue Operative Surgical Procedures Skin Wounds Xylazine Xylazine Hydrochloride
After 60 days of the ovariectomy (OVX) and simulated ovariectomy (Sham) procedures, the same rats were subjected to the manufacture of critical bone defects in the calvaria. The animals were weighed and anesthetized with a solution of xylazine hydrochloride 2.3 g/100 mL (Anasedan®, Vetbrands, Jacareí, Brazil) and ketamine hydrochloride 1.16 g/10 mL (Dopalen®, Vetbrands, Jacareí, Brazil).
After the anesthesia of the animals, the surgical sites were subjected to trichotomy and antisepsis with an iodized alcohol solution. A linear incision of approximately 3.5 mm was performed with a scalpel blade number 15 in the region corresponding to the medial face of the calvaria. The tissues were divulsed to expose the calvaria cortices, in which the 5.0 mm defects were made under abundant and continuous irrigation with physiological solution to prevent heating due to the friction of the drill with the bone. To obtain the critical defect, a 5.0 mm diameter drill was used. The defects on the left side in the OVX group were made with (a) bioglass (BG) or (b) bioglass associated with 10% teriparatide (BGT), and on right side, they were filled with clot. In the sham group, the defect was filled only by clot. For the stabilization of the material in the surgical site of the defect, a larger-diameter biological membrane (GenDerm®) was positioned at the bone defect site. In all defects, the tissues were repositioned, and the layers were sutured with no3 silk thread (Ethicon/Johnson & Johnson). Again, iodized alcohol antisepsis was performed. After surgery, the rats were placed in mini-isolate of the ventilated rack and monitored until the euthanasia deadline.
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Publication 2023
Anesthesia Animals Antisepsis Bioglass Biopharmaceuticals Bones Calvaria Clotrimazole Cortex, Cerebral Drill Ethanol Euthanasia Face Friction Ketamine Hydrochloride Operative Surgical Procedures Ovariectomy physiology Rattus norvegicus Silk Suby's G solution Teriparatide Tissue, Membrane Tissues Xylazine Hydrochloride

Top products related to «Antisepsis»

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Buprenorphine is a synthetic opioid compound that is used as a laboratory reagent and analytical standard. It is a Schedule III controlled substance in many countries. Buprenorphine is utilized in various research and analytical applications, but its detailed intended use cannot be provided in an unbiased and factual manner without further interpretation.
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Isoflurane is a volatile anesthetic agent used in the medical field. It is a clear, colorless, and nonflammable liquid that is vaporized and administered through inhalation. Isoflurane is primarily used to induce and maintain general anesthesia during surgical procedures.
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Rompun is a veterinary drug used as a sedative and analgesic for animals. It contains the active ingredient xylazine hydrochloride. Rompun is designed to induce a state of sedation and pain relief in animals during medical procedures or transportation.
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DNase I is an enzyme that catalyzes the hydrolytic cleavage of DNA. It is commonly used in molecular biology procedures to degrade DNA.
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Ketalar is a general anesthetic medication used to induce and maintain anesthesia. It is a clear, colorless, water-soluble compound that is administered via injection. The active ingredient in Ketalar is the chemical compound ketamine hydrochloride.
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ChloraPrep is a sterile skin preparation solution that contains chlorhexidine gluconate and isopropyl alcohol. It is designed for use in preparing the skin prior to surgery, intravenous catheter insertion, and other invasive medical procedures.
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MacConkey agar is a selective and differential culture medium used for the isolation and identification of Gram-negative enteric bacteria, particularly members of the Enterobacteriaceae family. It inhibits the growth of Gram-positive bacteria while allowing the growth of Gram-negative bacteria.

More about "Antisepsis"

Antisepsis is the process of preventing or reducing microbial contamination and infection, particularly in medical and surgical settings.
This critical process involves the use of antiseptic agents, procedures, and techniques to maintain a sterile or aseptic environment.
Effective antisepsis is crucial in healthcare to minimize the risk of hospital-acquired infections (HAIs) and promote patient safety.
Key aspects of antisepsis include hand hygiene, skin preparation, disinfection of medical equipment, and sterile technique during invasive procedures.
Researchers in the field of antisepsis can enhance their studies by utilizing PubCompare.ai, an AI-driven platform that helps identify optimal protocols from academic literature, preprints, and patents.
This powerful tool enables efficient comparison of antiseptic methods and products, boosting research productivity and success.
Related terms and concepts include Buprenorphine (a synthetic opioid analgesic), Isoflurane (a volatile anesthetic), Rompun (a sedative and analgesic for animals), DNase I (an enzyme used in molecular biology), PDS II (a suture material), Ketalar (the brand name for ketamine, a dissociative anesthetic), Ethyl ether (a volatile anesthetic), ChloraPrep (a skin antiseptic), FBS (fetal bovine serum, used in cell culture), and MacConkey agar (a selective and differential culture medium for Gram-negative bacteria).
By leveraging the power of AI-driven analysis and optimization tools like PubCompare.ai, researchers can enhance their antisepsis studies and drive success in this critical field of healthcare.