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Diet, High-Fat

A high-fat diet is a dietary pattern characterized by a high intake of fats, often exceeding 30% of total daily caloric intake.
This type of diet is commonly associated with research on metabolic disorders, weight management, and the impact of dietary fats on health.
High-fat diets may include a variety of fat sources, such as saturated, monounsaturated, and polyunsaturated fats, and can be studied in the context of animal models or human clinical trials.
Researchers leveraging high-fat diet protocols may focus on investigating the physiological effects, potential health benefits or risks, and underlying mechanisms involved in the body's response to this dietary approach.

Most cited protocols related to «Diet, High-Fat»

Adipocyte-Specific Irf4 Deletion

Cited 66 times

Protocol full text hidden due to copyright restrictions

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Publication 2011

Diet Diet, High-Fat Food Mice, Laboratory Therapy, Diet

Autophagy Regulation of Lipid Metabolism

Cited 60 times

The hepatocyte cell line RALA255-10G was cultured under nontransformed conditions, as described previously20 (link). Wild-type and Atg5^−/− mouse embryonic fibroblasts provided by N. Mizushima21 (link) were cultured as described previously22 (link). TG content was determined by the Trig/GB Kit (Roche Diagnostics), cholesterol content by the Amplex Red Cholesterol Assay (Invitrogen), fatty acid β-oxidation by a modification of a previously used method23 (link), and TG decay in cells radiolabelled with [¹⁴C]oleate and TG synthesis by standard methods12 (link). shRNAs were cloned into pSUPER (Ambion) and then pCCL.sin.PPT.hPGK.GFPWpre24 (link). Protein isolation and western blotting were performed as described previously25 (link). Fluorescence microscopy for BODIPY 493/503 (Invitrogen) and immunofluorescence were performed as described previously26 (link). Atg7^F/F mice4 (link) were crossed with Alb-Cre mice27 (link) to generate Atg7^F/F-Alb-Cre mice. Some animals were fed a high-fat diet (60% kcal in fat; Research Diets, D12492). Electron microscopy and immunogold labelling were performed as described previously26 (link). LDs from mouse livers were isolated by sucrose density gradient centrifugation28 and autophagic vacuoles and lysosomes by centrifugation in metrizamide discontinuous density gradients29 (link).

Singh R., Kaushik S., Wang Y., Xiang Y., Novak I., Komatsu M., Tanaka K., Cuervo A.M, & Czaja M.J. (2009). Autophagy regulates lipid metabolism. Nature, 458(7242), 1131-1135.

Publication 2009

4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene Anabolism Animals Autophagosome Biological Assay Cell Lines Cells Centrifugation Cholesterol Diagnosis Diet Diet, High-Fat Electron Microscopy Embryo Fatty Acids Fibroblasts formaldehyde-serum albumin Hepatocyte Immunofluorescence isolation Liver Lysosomes Metrizamide Mice, Laboratory Microscopy, Fluorescence Oleate Proteins Short Hairpin RNA Sucrose

Filtering Microbiome Datasets for Microbe-Metabolite Interactions

Cited 37 times

Due to the overwhelming sparsity in microbiome datasets, some filtering is required in order to infer microbe-metabolite interactions. We chose to filter out microbes that appear in less than 10 samples, since these microbes don’t have enough information to infer which metabolites are co-occurring with them. In other words the mmvec model has too many degrees of freedom to perform inference on these microbes. For the cystic fibrosis study, there were 172 samples and after filtering there were 138 unique microbial taxa and 462 metabolite features. For the biocrust soils study, there were 19 samples and after filtering there were 466 unique microbial taxa and 85 metabolite features. For the murine high fat diet study, there were 434 samples and after filtering there were 902 microbes and 11978 metabolites. For the IBD dataset, there were 13920 features in the c18 LCMS dataset, 26966 features in the c8 LCMS dataset and 562 taxa. Cross validation was performed across all studies to evaluate overfitting. In the desert biocrust soils experiment, 1 sample out of 19 samples was randomly chosen to be left out for cross-validation. In all of the other studies, 10 samples were randomly chosen to be left out for cross-validation. All of the analyses can be found under https://github.com/knightlab-analyses/multiomic-cooccurences.

Morton J.T., Aksenov A.A., Nothias L.F., Foulds J.R., Quinn R.A., Badri M.H., Swenson T.L., Van Goethem M.W., Northen T.R., Vazquez-Baeza Y., Wang M., Bokulich N.A., Watters A., Song S.J., Bonneau R., Dorrestein P.C, & Knight R. (2019). Learning accurate representations of microbe-metabolite interactions. Nature methods, 16(12), 1306-1314.

Publication 2019

Cystic Fibrosis Diet, High-Fat Laser Capture Microdissection Microbial Interactions Microbiome Mus

Dietary Modulation of GPR120 Signaling

Cited 37 times

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Publication 2010

AD 20 Animals Body Weight Diet, High-Fat Fishes Food Males Menhaden oil Mice, House Oils, Fish Specific Pathogen Free Therapy, Diet

MicroRNA Regulation of Atherosclerosis Development

Cited 30 times

Pre-miR miRNA precursor molecules negative (nonspecific) control #1 (AM17110) and Hsa-miR-181b-5p Pre-miR miRNA precursor (PM12442) were used from Ambion. Real-time quantitative polymerase chain reaction was performed with the Mx3000P real-time polymerase chain reaction system (Stratagene) following the manufacturer's instructions. NF-κB promoter with GFP/luciferase fusion reporter (NGL) mice fully backcrossed into C57BL/6 were crossed with homozygous ApoE^−/− mice to generate ApoE^−/−/NGL transgenic mice. To induce atherosclerosis, 8-week-old male ApoE^−/− mice were fed a high-fat diet (HFD) from Research Diets Inc (D12108Ci) for 12 weeks. Aortas were carefully excised from mice and examined for immunohistology and characterization of atherosclerotic lesions.
For detailed experimental methods, please see the Online Data Supplement.

Sun X., He S., Wara A.K., Icli B., Shvartz E., Tesmenitsky Y., Belkin N., Li D., Blackwell T.S., Sukhova G.K., Croce K, & Feinberg M.W. (2013). Systemic Delivery of MicroRNA-181b Inhibits Nuclear Factor-κB Activation, Vascular Inflammation, and Atherosclerosis in Apolipoprotein E–Deficient Mice. Circulation research, 114(1), 32-40.

Publication 2013

Aorta Apolipoproteins E Atherosclerosis Diet Diet, High-Fat Dietary Supplements Homozygote Luciferases Males Mice, Laboratory Mice, Transgenic MicroRNAs miR-181b, human pre-miRNA Real-Time Polymerase Chain Reaction RELA protein, human

Most recents protocols related to «Diet, High-Fat»

Oxyntomodulin Derivative-Fc Conjugates Reduce Body Weight

Not available on PMC !

Example 18

It was examined whether the oxyntomodulin derivative-immunoglobulin Fc conjugates show excellent body weight-reducing effects in vivo.

Specifically, 6-week-old normal C57BL/6 mice were fed a high fat diet of 60 kcal for 24 weeks to increase their body weight by approximately 50 g on average, and subcutaneously administered with oxyntomodulin derivative (SEQ ID NO: 23, 24 or 25)-immunoglobulin Fc conjugates at a dose of 0.03 or 0.06 mg/kg/week for 3 weeks. Thereafter, changes in the body weight of the mice were measured (FIG. 12 and FIG. 13). FIG. 12 and FIG. 13 are graphs showing changes in body weight of mice according to the type and administration dose of oxyntomodulin derivative-immunoglobulin Fc conjugates. As shown in FIG. 12 and FIG. 13, as the administration dose of the oxyntomodulin derivative-immunoglobulin Fc conjugates was increased, the body weight was reduced in direct proportion, even though there were differences between the types of the oxyntomodulin derivative-immunoglobulin Fc conjugates, suggesting that the oxyntomodulin derivative-immunoglobulin Fc conjugates reduce the body weight in a dose-dependent manner.

US11872283B2. Conjugate comprising oxyntomodulin and an immunoglobulin fragment, and use thereof (2024-01-16). HANMI SCIENCE CO., LTD [KR]. Inventors: Sung Youb Jung [KR], Dae Jin Kim [KR], Sung Hee Park [KR], Young Eun Woo [KR], In Young Choi [KR], Se Chang Kwon [KR].

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Patent 2024

Body Weight Diet, High-Fat Human Body Immunoglobulin Fc Fragments Immunoglobulins Mice, Inbred C57BL Mus Oxyntomodulin

Obesity and Adipocyte ACAT Expression

Not available on PMC !

Example 1

To examine the function of ACATs in obesity, the expression patterns of ACAT1 and ACAT2 genes, and their gene products during adipogenesis of murine 3T3-L1 preadipocytes in vitro were examined. ACAT1 mRNA level was markedly increased in adipocytes from 2 days after initiation of adipogenesis (i.e., D2) as judged by real-time PCR assay (FIG. 1). However, ACAT2 mRNA level was similar between preadipocytes (D0) and mature adipocytes (D6) while a temporal reduction of ACAT2 level was observed at D2 (FIG. 1). In addition, white adipose tissue (WAT) isolated from high fat diet-induced obese mice displayed elevated mRNA level of ACAT1 and reduced mRNA level of ACAT2 when compared with those in lean mice as judged by real-time PCR assay. Leptin level was measured in WAT from lean and obese mice to ensure the development of obesity (FIG. 2). In addition, brown adipose tissue (BAT) from obese mice exhibited elevated levels of both ACAT1 and ACAT2. Uncoupling protein-1 (UCP-1) level was measured in BAT from lean and obese mice as a BAT-specific marker protein (FIG. 2). However, liver from lean and obese mice exhibited similar levels of ACAT1 and ACAT2 (FIG. 2).

US11872198B2. Compositions and methods for regulating body weight and metabolic syndromes (2024-01-16). Purdue Research Foundation [US]. Inventors: Kee-Hong Kim [US], Yuyan Zhu [US].

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Patent 2024

3T3-L1 Cells Adipocytes Adipogenesis a protein, mouse Biological Assay Brown Adipose Tissue Uncoupling Protein Brown Fat CES1 protein, human Diet, High-Fat Genes Leptin Liver Mice, Obese Mus Obesity Proteins Real-Time Polymerase Chain Reaction RNA, Messenger White Adipose Tissue

Milk Fatty Acid Composition Analysis

There were no significant differences between results from recordings or analyses from samples obtained on lactation d 10 and d 17, except for piglet performance. The mean of values observed for d 10 and d 17 were therefore used in the statistical analysis. All data were analyzed using the PROC MIXED procedure of SAS (version 9.4, SAS Institute Inc). For gene expression data, the delta Ct values (ΔCt = Ct of the target gene – mean Ct of the reference genes) of genes were used for statistical analysis. All the data were analyzed in 2 ways: as orthogonal contrasts between low-fat and the four high-fat diets, and by multiple comparisons between the four high-fat diets. Treatment differences between groups were determined using the PDIFF option, and the results were expressed as least-squares means with pooled-standard error, except for the genes relative abundance, which were reported as a mean ± 95% confidence limits. The CORR procedure of SAS was used to analyze the correlations of digestible FA intake and milk FA output, and the amounts of de novo synthesized fat using the two prediction methods. For the statistical evaluation, P ≤ 0.05 was declared as a significant response, while P ≤ 0.10 was declared as a tendency.

Zhe L., Krogh U., Lauridsen C., Nielsen M.O., Fang Z, & Theil P.K. (2023). Impact of dietary fat levels and fatty acid composition on milk fat synthesis in sows at peak lactation. Journal of Animal Science and Biotechnology, 14, 42.

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Publication 2023

Breast Feeding Contrast Media Diet, High-Fat Fat-Restricted Diet Gene Expression Genes Milk

Murine NAFLD and Atherosclerosis Protocol

All animal procedures were performed under a project licence (PPL P94B395E0) approved by the U.K. Home Office under the Animals (Scientific Procedures) Act 1986 and the University of Aberdeen ethics review board. Studies were performed following the recommendations in the ARRIVE guidelines under guidance by the Veterinary Surgeon and Animal Care and Welfare Officers of the institutional animal research facility. Thus, all methods were performed in accordance with the relevant guidelines and regulations. Male LDLR^−/− mice, aged 4–6 weeks, were purchased from The Jackson Laboratory (supplied by Charles River UK Ltd), male and female ApoE^−/− mice were bred in-house (University of Aberdeen). All mice were fed chow diet until 12 weeks of age then placed into three groups and fed the following diets (all Research Diets Inc.) to induce atherogenesis and NAFLD for 14 weeks: control (10% kCal fat D14121001) or high-fat/high-cholesterol diet (HFD, 40% kCal fat from cocoa butter and soybean oil, 34.5% kcal and 5.5% kcal respectively, plus 1.25% cholesterol, Clinton/Cybulsky D12108C) + /- 0.04% Fenretinide (FEN-HFD, D18061502,^{16 (link),27 (link)–29 (link)}). Mice were maintained at 22–24 °C on 12-h light/dark cycle with free access to food/water. At week 14, mice were fasted for 5 h and injected intraperitoneally with either saline or insulin (10 mU/g body weight) for 10 min prior to CO₂-induced anaesthesia followed by cervical dislocation. Heart and aortic tissues were collected for histological analysis. Peripheral metabolic tissues (liver, muscle and white adipose tissue (WAT)) were frozen in liquid nitrogen and stored at − 80 °C until subsequent analysis.

Thompson D., Mahmood S., Morrice N., Kamli-Salino S., Dekeryte R., Hoffmann P.A., Doherty M.K., Whitfield P.D., Delibegović M, & Mody N. (2023). Fenretinide inhibits obesity and fatty liver disease but induces Smpd3 to increase serum ceramides and worsen atherosclerosis in LDLR−/− mice. Scientific Reports, 13, 3937.

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Publication 2023

Anesthesia Animals Aorta Apolipoproteins E Atherogenesis Body Weight Cholesterol cocoa butter Diet Diet, High-Fat Females Fenretinide Food Freezing Heart Insulin Joint Dislocations LDLR protein, human Liver Males Mice, House Muscle Tissue Neck Nitrogen Non-alcoholic Fatty Liver Disease Rivers Saline Solution Soybean oil Surgeons Therapy, Diet Tissues White Adipose Tissue

Alzheimer's Disease Comorbidity Study in Mice

All experiments detailed herein complied with the regulations formulated by the Institutional Animal Care and Use Committee (IACUC) of the Weizmann Institute of Science (application numbers: 03960618-3, 01200121-2, 03230322-2). Female and male mice were bred and maintained by the Animal Breeding Center of the Weizmann Institute of Science. Housing conditions were: 12-hour dark/light cycle (lights on at 8 am), temperature 22 °C, humidity 30-70%. For comorbidity studies, heterozygous 5xFAD transgenic mice^{33 (link)} (line Tg6799, The Jackson Laboratory) on a C57/BL6-SJL background and age-matched wild-type (WT) controls were used. Genotyping was performed by PCR analysis of ear clipping DNA, as previously described^{33 (link)}. Since the C57/BL6-SJL strain carries the retinal degeneration Pde6b^rd1 mutation, which causes visual impairment in homozygosis (https://www.jax.org/strain/100012), mice were further tested for presence of the allele, as previously described^{90 (link)}. To avoid gut microbiota-related cage effects due to coprophagia^{91 (link)}, 5xFAD and WT mice were housed together. For the study of the effects of NANA on the immune system in vivo, we used four cohorts of female and male WT mice, and specifically: three cohorts of C57/BL6-SJL mice, age 6.5, 9, and 14 mo; one cohort of C57/BL6 mice, age 11 mo. For the study of the effects of NANA on novelty discrimination, female C57/BL6-SJL 5xFAD and age-matched WT controls were used. To avoid NANA assimilation with coprophagia, NANA-injected mice were housed separately from the PBS-injected controls. All mice were provided with standard chow (calories from proteins: 24%; calories from carbohydrates: 58%; calories from fat: 18%; 2918, Teklad), placed on a hopper integrated with the cage lid, and water ad libitum, and housed in cages enriched with one paper shelter. For comorbidity studies, to induce obesity, at 6-9 weeks of age, mice were switched to a high-fat diet (HFD; calories from proteins: 18%; calories from carbohydrates: 22%; calories from fat: 60%; TD.06414, Teklad), and the food pellet checked twice a week for replenishment. Control mice were kept on standard chow (control diet, CD). Mice allocated for behavioral studies or NANA/PBS injections were switched to a 12-h reversed dark/light cycle (lights on at 8 pm) at least 7 days prior to behavior assessment, and maintained in the regimen until experimental endpoint.

Suzzi S., Croese T., Ravid A., Gold O., Clark A.R., Medina S., Kitsberg D., Adam M., Vernon K.A., Kohnert E., Shapira I., Malitsky S., Itkin M., Brandis A., Mehlman T., Salame T.M., Colaiuta S.P., Cahalon L., Slyper M., Greka A., Habib N, & Schwartz M. (2023). N-acetylneuraminic acid links immune exhaustion and accelerated memory deficit in diet-induced obese Alzheimer’s disease mouse model. Nature Communications, 14, 1293.

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Publication 2023

Alleles Animals Animals, Transgenic Carbohydrates Coprophagia Diet, High-Fat Discrimination, Psychology Females Food Gastrointestinal Microbiome Heterozygote Homozygote Humidity Institutional Animal Care and Use Committees Light Low Vision Males Mice, Laboratory Mutation Obesity Proteins Retinal Degeneration Strains System, Immune Therapy, Diet Treatment Protocols

Top products related to «Diet, High-Fat»

D12492 by Research Diets

Sourced in United States, China, Canada, Japan, Denmark, Montenegro, United Kingdom

The D12492 is a powdered rodent diet formulated by Research Diets. It is a highly palatable, nutrient-dense diet that provides a standardized nutritional profile for research purposes. The diet is designed to be easily administered and consumed by laboratory rodents.

D12451 by Research Diets

Sourced in United States, China, Germany, Canada, Japan, United Kingdom

D12451 is a standard rodent chow formulated to provide a controlled amount of calories, macronutrients, and micronutrients for research purposes. It is a nutritionally complete diet suitable for maintaining rodents in a laboratory setting. The product details and nutrient composition are available upon request.

High fat diet (hfd) by Research Diets

Sourced in United States, China, Switzerland, Canada, Denmark, Japan, Germany

The HFD is a high-fat diet formulation designed for research purposes. It provides a specified nutritional composition to support dietary studies. The core function of the HFD is to deliver a controlled high-fat diet to laboratory animals.

Streptozotocin (stz) by Merck Group

Sourced in United States, Germany, China, Sao Tome and Principe, United Kingdom, India, Japan, Macao, Canada, France, Italy, Switzerland, Egypt, Poland, Hungary, Denmark, Indonesia, Singapore, Sweden, Belgium, Malaysia, Israel, Spain, Czechia

STZ is a laboratory equipment product manufactured by Merck Group. It is designed for use in scientific research and experiments. The core function of STZ is to serve as a tool for carrying out specific tasks or procedures in a laboratory setting. No further details or interpretation of its intended use are provided.

C57bl 6j mice by Jackson ImmunoResearch

Sourced in United States, Montenegro, Japan, Canada, United Kingdom, Germany, Macao, Switzerland, China

C57BL/6J mice are a widely used inbred mouse strain. They are a commonly used model organism in biomedical research.

Td 88137 by Inotiv

Sourced in United States, United Kingdom, Montenegro

The TD.88137 is a laboratory equipment product from Inotiv. It is designed for use in scientific research and analysis applications. The core function of the TD.88137 is to perform a specific task or measurement, but a detailed description is not available while maintaining an unbiased and factual approach.

C57bl 6j by Jackson ImmunoResearch

Sourced in United States, Montenegro, Germany, United Kingdom, Japan, China, Canada, Australia, France, Colombia, Netherlands, Spain

C57BL/6J is a mouse strain commonly used in biomedical research. It is a common inbred mouse strain that has been extensively characterized.

C57bl 6j mice by Charles River Laboratories

Sourced in China, Japan, Germany, France, United Kingdom, United States, Italy, Canada, Montenegro, Belgium, Morocco, Netherlands, Spain

The C57BL/6J mouse is a widely used laboratory mouse strain. It is an inbred strain that has a black coat color. The C57BL/6J mouse is commonly used as a control strain in various research applications.

C57bl 6 mice by Jackson ImmunoResearch

Sourced in United States, Montenegro, Canada, China, France, United Kingdom, Japan, Germany

C57BL/6 mice are a widely used inbred mouse strain commonly used in biomedical research. They are known for their black coat color and are a popular model organism due to their well-characterized genetic and physiological traits.

C57bl 6 mice by Charles River Laboratories

Sourced in China, United States, Germany, United Kingdom, Canada, Japan, France, Italy, Morocco, Spain, Netherlands, Montenegro, Belgium, Portugal, Ireland, Hungary

The C57BL/6 mouse is a widely used inbred mouse strain. It is a common laboratory mouse model utilized for a variety of research applications.

What are the key benefits of a high-fat diet?

A high-fat diet can offer several potential benefits, including supporting weight management, improving metabolic health, and providing a sustainable energy source. However, the specific effects can vary depending on the types of fats consumed, the overall dietary composition, and individual factors. It's important to work with a healthcare professional to determine if a high-fat diet is appropriate and to ensure balanced nutrient intake.

What are the common types of high-fat diets?

High-fat diets can take various forms, such as the ketogenic diet, the Atkins diet, and the Mediterranean diet. These diets often differ in their macronutrient ratios, the types of fats emphasized, and the overall dietary approach. It's important to understand the nuances of each high-fat diet variation to determine the best fit for your specific health and fitness goals.

How can PubCompare.ai help with high-fat diet research?

PubCompare.ai can be a valuable tool for researchers studying high-fat diets. The platfrom allows you to efficiently screen protocol literature, leveraging AI to pinpoint critical insights. By comparing the effectiveness of different high-fat diet protocols, PubCompare.ai can help you identify the most suitable options for your specific research goals, improving reproducibility and accuracy. The platform's AI-driven analysis can highlight key differences in protocol effectiveness, enabling you to make informed decisions and achieve better results.

What are some common challenges with implementing a high-fat diet?

Implementing a high-fat diet can present some challenges, such as adjusting to the macronutrient shift, managing satiety and hunger, and ensuring adequate nutrient intake. Indivduals may also experience side effects like fatigue, digestive issues, or changes in energy levels during the transition period. Workinbg closely with a healthcare provider can help address these challenges and ensure the diet is tailored to your individual needs.

More about "Diet, High-Fat"

High-fat diets (HFDs), also known as high-fat, low-carb (HFLC) diets, are dietary patterns characterized by a significant intake of fats, often exceeding 30% of total daily caloric consumption.
These diets have become a subject of intense research, particularly in the context of metabolic disorders, weight management, and the impact of dietary lipids on overall health.
HFDs can encompass a diverse range of fat sources, including saturated, monounsaturated, and polyunsaturated fats, and are often studied in both animal models (e.g., C57BL/6J mice) and human clinical trials.
Researchers leveraging HFD protocols may focus on investigating the physiological effects, potential health benefits or risks, and underlying mechanisms involved in the body's response to this dietary approach.
In addition to HFDs, related terms and concepts such as D12492, D12451, streptozotocin (STZ), and the TD.88137 diet formulation are also relevant to this area of research.
These specialized diets and compounds are commonly used to induce metabolic disturbances, such as obesity and diabetes, in animal models to study the impact of dietary factors on disease pathogenesis and progression.
By optimizing your high-fat diet research with AI-driven platforms like PubCompare.ai, you can enhance the reproducibility and accuracy of your studies.
This tool helps you easily locate relevant protocols from the literature, preprints, and patents, while leveraging AI-driven comparisons to identify the best protocols and products for your high-fat diet investigations.
Acheive better results and gain deeper insights into the complexities of dietary fat metabolism and its implications for health and disease.