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Diet, High-Protein

High-Protein Diet: An eating plan that emphasizes proteins as the primary nutrient, often with a reduced intake of carbohydrates.
This type of diet is commonly used to support weight loss, muscle growth, and overall health.
The high-protein content can come from a variety of sources, such as lean meats, fish, eggs, dairy, and plant-based options like beans, lentils, and soy.
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Most cited protocols related to «Diet, High-Protein»

In the randomized maintenance phase, which was initiated immediately after participants completed the weight-loss phase, participants were assigned to one of five diets, in a two-by-two factorial design: a diet that was low in protein (13% of total energy consumed) with a low glycemic index, a diet that was low in protein with a high glycemic index, a diet that was high in protein (25% of total energy consumed) with a low glycemic index, a high-protein and high-glycemic-index diet, or a control diet. The control diet, which followed dietary guidelines in each participating country, had a moderate protein content and did not include instructions to participants with respect to the glycemic index.
Study participants were instructed to maintain their weight loss during this phase, although further weight reduction was allowed. All five diets were designed to have a moderate fat content (25 to 30% of total energy consumed) with no restrictions on energy intake (i.e., ad libitum diets), in order to test the ability of the diets to regulate appetite and body weight. We targeted a difference of 15 glycemic-index units between the high-glycemic-index diets and the low-glycemic-index diets and a difference of 12% of total energy consumed from protein between the high-protein diets and the low-protein diets. Visits for dietary counseling took place every other week during the first 6 weeks and monthly thereafter. The families were provided with recipes, cooking and behavioral advice, and a point-based teaching system to achieve the targeted macronutrient compositions.13 (link)In Maastricht and Copenhagen (“shop centers”), the families received dietary instruction plus free foods from the laboratory shop for 26 weeks so that we could assess the effect that the provision of food would have on adherence. In the other six centers (“instruction centers”), the families were provided with dietary instruction only.14 (link),15 (link) Local sponsors made financial contributions to the shop centers, and food manufacturers provided a number of foods free of charge. The local sponsors and food manufacturers had no influence on the selection of foods found in the two shops, nor were they involved in designing the study or in analyzing and interpreting data.
Publication 2010
Body Weight Diet Diet, High-Protein Diet, Protein-Restricted Food Hyperglycemia Hypoglycemia Macronutrient Proteins Therapy, Diet
The aim is to determine the effects and interactions of two diets and two physical activity programmes on the prevention of T2D in overweight, pre-diabetic adults, who have undergone a short period of significant weight loss. Our primary hypothesis is that a higher protein, lower CHO/low GI diet (based on the DiOGenes study [19 (link)]) will be superior in preventing T2D when compared with a moderate protein, higher CHO/moderate GI diet (based on the DPS and DPP studies [10 (link),14 (link)]). We also hypothesise that high-intensity physical activity will be superior compared to moderate-intensity physical activity [25 (link)].
Each participant receives one of the two dietary programs, and one of the two physical activity programs, thus, we have four groups (high protein diet and high-intensity physical activity; moderate protein diet and high-intensity physical activity; high protein diet and moderate-intensity physical activity; moderate-protein diet and moderate-intensity physical activity). The majority of outcomes will be analysed by using these four arms. The primary endpoint and statistical power calculations are based on a two-arm design (diets compared against each other).
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Publication 2017
Adult Diet Diet, High-Protein Proteins Staphylococcal Protein A States, Prediabetic
Subjects kept a daily diary of their food intake via a smartphone app (MyFitnessPal®). The use of mobile apps for diet self-monitoring have been previously used [15 (link)]. If they did not use the mobile app, subjects instead kept a paper diary and their daily food intake was measured via the Nutribase® program. In order to maintain a high protein diet, subjects consumed commercially available whey and casein protein powder (MusclePharm® and Adept Nutrition [Europa®]). Otherwise, the rest of their dietary protein was obtained from their normal food intake.
Height was measured using standard anthropometry and total body weight was measured using a calibrated scale. Body composition was assessed by whole body densitometry using air displacement via the Bod Pod® (COSMED USA, Concord, CA). All testing was performed in accordance with the manufacturer’s instructions. Briefly, subjects were tested while wearing only tight fitting clothing (swimsuit or undergarments) and an acrylic swim cap. The subjects wore the exact same clothing for all testing. Thoracic gas volume was estimated for all subjects using a predictive equation integral to the Bod Pod® software. The calculated value for body density used the Siri equation to estimate body composition. Data from the Bod Pod® included body weight, percent body fat, fat free mass and fat mass. All testing was done with each subject at approximately the same time of day. Also, all subjects were required to keep a daily workout log showing the exercises with reps and sets performed. Volume load (repetitions × weight) was measured to ensure subjects did not alter their training regimen.
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Publication 2014
Body Composition Body Fat Caseins Densitometry Diet Diet, High-Protein Dietary Proteins Eating Human Body Powder Proteins Seizures Treatment Protocols Whey Proteins
Total bacterial DNA extractions from 500 mg faeces were performed according to Boon et al. [33 (link)]. Isolated DNA was subsequently used as a template to amplify the 16S rDNA for all members of the Bacteria with forward primer P338F-GC and the reverse primer P518r, and a GC-clamp of 40 bp was incorporated into the forward primer. DGGE based on the protocol of Muyer et al. [34 (link)] was performed on the Bio-Rad D gene system (Bio-Rad). The PCR products (10 μL of mixture from 20 μL PCR product and 5 μL loading dye) of the second round were loaded. The obtained DGGE patterns were normalized and analyzed using BioNumerics 2.0 (Applied Maths) [35 (link)]. The number of bands in the DGGE profile was used to calculate the richness in the present study. A matrix of similarities for the densiometric curves of the band patterns was calculated based on the Pearson product-moment correlation coefficient, and dendrograms were created by using Ward linkage [36 (link)].
The quantification of DNA by qPCR was performed with a C1000 Thermal Cycler (Bio-Rad). The amplification and detection were carried out in 96-well plates using SensiMixTM SYBR No-ROX Kit (Bioline Reagents Ltd). Each reaction was done in triplicate in 12 μL total reaction mixture using 2 μL of 50 ng of the DNA sample except for BK where 2 μL of undiluted DNA was used. All qPCR results were expressed as gene copies per g of fresh faeces. The primer sets used in this study are listed in Table 2. A melting curve analysis was done after amplification to confirm specificity of the reaction. Quantification was done by using standard curves made from known concentrations of plasmid DNA containing the respective amplicon for each set of primers.

Ingredient composition and nutrient analysis of the experimental diets

ItemsLPHP
Ingredients
As-is basis (g/100 g)
Pork greaves11.053.3
Brewers rice50.920.0
Lard11.08.50
Rice meal15.06.00
Beet pulp3.705.00
Dicalcium phosphate3.002.10
Yeast1.001.00
Salmon Oil1.001.00
Animal digest10.890.89
Calcium carbonate0.400.50
Bentonite clay0.500.50
Salt0.810.42
Vitamin mix0.260.23
Mineral mix0.220.22
Chorine chloride0.140.14
Lecithine0.100.10
Nutrient Analysis
DM (g/100 g)92.796.2
g/100 g DM
Ash5.354.75
CP17.850.0
EE13.612.2
CF1.050.91
NFE262.332.2
Insoluble fibre2.648.28
Soluble fibre2.150.27
TDF4.798.56
ME, kJ/100gDM318501833

LP: low protein diet; HP: high protein diet; CP: crude protein; DM: dry matter; EE: ether extract; CF: crude fibre; ME: metabolizable energy; NFE: nitrogen-free extract; TDF: total dietary fibre

1Animal digest: a material which results from chemical and/or enzymatic hydrolysis of clean and undecomposed animal tissue [66 (link)]

2Calculated %NFE = % DM – (% EE+ % CP + % ash + % CF)

3Calculated ME = 16.7×g CP + 37.7×g Fat +16.7×g NFE [27 ]

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Publication 2017
Animals Bacteria Denaturing Gradient Gel Electrophoresis Diet Diet, High-Protein Diet, Protein-Restricted DNA, Bacterial Enzymes Ethyl Ether Feces Fibrosis Genes Genitalia Hydrolysis Nitrogen Nutrients Oligonucleotide Primers Plasmids Pork Proteins Recombinant DNA Tissues
Subjects kept a daily diary of their food intake via a smartphone app (MyFitnessPal®). The use of mobile apps for self-monitoring of diets has been previously used [8 (link)]. Virtually every subject had previously used this mobile app. Thus familiarity was not an issue except for a small minority. These individuals were taught by the investigators how to properly input data into the app. Note that the MyFitnessPal® app is a database comprised of over 5 million foods that have been provided by users via entering data manually or by scanning the bar code on packaged goods. Thus, the data themselves are primarily derived from food labels (i.e., Nutrition Facts Panel) derived from the USDA National Nutrient database. Thus, in order for subjects to consume a high protein diet, whey or beef protein powder was provided at no cost to the research subjects. However, they were not required to consume protein powder. The rest of their dietary protein was obtained from their regular food intake.
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Publication 2015
Beef Diet Diet, High-Protein Dietary Proteins Eating Food Food Labeling Minority Groups Nutrients Powder Proteins Teaching Whey Proteins

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Publication 2023
Animals Diet, High-Protein Fruit Humidity Immunoglobulins Institutional Animal Care and Use Committees Macaca mulatta Males Monkeys Nuts Perch Secondary Immunization Vaccination Vegetables
The study consisted of (i) a cross-sectional, online questionnaire and (ii) a phone interview, conducted amongst individuals with a diagnosis, history or elevated risk of CVD, with questions focused on a proposed high-protein Mediterranean-style diet and RE intervention for CR patients (37 (link)). This methodology was used due to COVID-19-related social distancing restrictions implemented in the UK at the time of data collection.
The principles of a “person-based” approach were used to help refine and evaluate the proposed intervention. Such a person-based approach may help those designing the intervention to better understand how potential participants, as individuals, react to the proposed methodology and identify which aspects may need to be refined for a more feasible implementation (36 (link)). Core-elements of such an approach include (i) intervention planning, (ii) design, and (iii) evaluation of acceptability (36 (link)). The initial planning and design of the proposed intervention were carried out in 2019 with the assistance of the Liverpool Heart and Chest Hospital (LHCH) Service Users Research Endeavour (SURE) group1, a PPI group, and CR staff from LHCH Knowsley Community Cardiovascular Service (KCCS).
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Publication 2023
Cardiovascular System Chest COVID 19 Diagnosis Diet, High-Protein Heart Patients Personnel, Hospital
Thirty female five-to-eight-day old European starlings (Sturnus vulgaris) were collected from nest boxes in late-April to early-May 2015 from a native colony in Upper Bavaria, South Germany (47° 58′ N, 11° 13′ 142 E) and brought into an animal care facility at the Max Planck Institute for Ornithology (MPIO), Seewiesen, Germany. We chose to focus on female starlings as they may face unique physiological challenges during migration in relation to their reproductive success. Migratory birds prepare to breed shortly after spring migration and depositing antioxidants into eggs has been shown to lead to higher hatching and fledging rates for offspring [128 (link), 129 (link)]. Female birds may thus face substantial oxidative tradeoff during migration, as they must balance preventing oxidative damage with their future reproductive success [44 (link)]. Hatchlings were hand-raised and fed a high protein diet consisting of bee larvae, crickets, wax worms, green bottle fly larvae (pinkies and buffaloes), beef heart with vitamin mixture and calcium carbonate powder. Once hatchlings were able to feed independently, we additionally offered them live mealworms, fresh fruits and vegetables. The diet has been successfully used to hand-raise starlings at the MPIO in previous experiments [32 (link), 73 (link), 130 (link)]. From about the age of 35–100 days, the starlings were moved to outdoor aviaries, kept under a natural light cycle, and maintained on an MPIO diet that consisted of insect powder, lettuce, fresh apples and oranges, dried fruit pellets, and mealworms.
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Publication 2023
Animals Antioxidants Aves Beef Buffaloes Carbonate, Calcium Diet Diet, High-Protein Eggs Europeans Face Females Fruit Gryllidae Heart Helminths Insecta Lactuca sativa Larva Maggots Oxidative Damage Pellets, Drug physiology Powder Reproduction Sturnidae Tenebrio Vegetables Vitamins
This prospective cohort study recruited 224 patients who began HD therapy at the Blood Purification Center, Zhongshan Hospital, Fudan University, from January 1, 2010 to October 31, 2012. Exclusion criteria: < 18 years of age, rapidly progressive kidney disease, history of chronic rheumatic heart disease, chronic liver disease, cancer, kidney transplantation, and peritoneal dialysis. All patients were of Chinese origin. The clinical data included age, sex, body mass index (BMI), smoking history and comorbidities such as hypertension (HBP), diabetes (DM), and CVDs. Patients were treated three times per week (4 h per session) with standard bicarbonate dialysate (Na+ 138.0 mmol/L, HCO3 32.0 mmol/L, K+ 2.0 mmol/L, Ca2+ 1.25 mmol/L, Mg2+ 0.5 mmol/L) by low-flux haemodialysis using 1.4-m2 dialyzers with synthetic membranes (BLS514SD;Sorin Group Italia, Mirandola, Italy and Polyflux 14L; Gambro Dialysatoren GmbH, Hechigen, Germany). The blood flow was 200–300 ml/min, and the dialysate flow was 500 ml/min. The water quality conformed to the Association for the Advancement of Medical Instrumentation standard and was examined every month. During the study, dry weight was reevaluated every month to guarantee a dry weight in every patient. In our centre, all patients on haemodialysis were advised to have a high-protein diet (at least 1.2 g/kg per day with mainly animal protein).
This study was approved by the ethics committee, Zhongshan Hospital, Fudan University, and all the patients provided written informed consent.
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Publication 2023
Animals Bicarbonates Birth BLOOD Blood Circulation Chinese Diabetes Mellitus Dialysis Solutions Diet, High-Protein Disease, Chronic Ethics Committees Hemodialysis High Blood Pressures Index, Body Mass Kidney Diseases Kidney Transplantation Liver Liver Diseases Malignant Neoplasms Patients Peritoneal Dialysis Proteins Rheumatic Heart Disease Therapeutics Tissue, Membrane
To gain insight in the metabolic capabilities of the microbe-microbe interactions we used community modelling. Community models for each patient in the metformin cohort were reconstructed using the MIGRENE toolbox57 . A maximum of 10 MSPs per community was reconstructed due to computational power requirements and to assure model functionality. The S matrices were combined so that each microbe has their own cellular compartment. Each microbial cell has a compartment which represents the intestinal lumen where metabolites from food ingestion is present. Another compartment is present for secreted microbial-metabolites and remaining food-derived metabolites that are not consumed by microbes. These microbial metabolites can be absorbed and reach blood circulation or excreted and present in human faeces. For each community, each individual bacterium biomass function was constrained on the respective abundance in each specific sample. Community biomass is composed of all the different microbes present in the community. FBA was performed with biomass as the objective function. The communities were constrained based on multiple diets (high protein omnivorous, high protein plant-based, high fiber plant-based, high fiber omnivorous, western diet and ketogenic diet) in anaerobic conditions. Models that had similar number of productions across communities in both groups (M0 and M4) were ignored, in order to identify main contributors to increased or decreased secretions after metformin treatment (M4). Only microbial models with production in at least 3 communities in the group were considered.
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Publication 2023
Bacterial Physiological Phenomena Blood Circulation Cells Diet Diet, High-Protein Feces Fibrosis Food Homo sapiens Intestines Ketogenic Diet Metformin Microbial Interactions MST1 protein, human Patients Plants Secretions, Bodily

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D10001 is a precision diet dispenser designed for controlled feeding studies. It accurately dispenses a specified amount of rodent diet into food cups or hoppers. The device features programmable portion sizes and delivery schedules to facilitate consistent and regulated feeding regimens.
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More about "Diet, High-Protein"

High-Protein Diets: Optimizing Nutrition and Health.
A high-protein diet, also known as a protein-rich diet or a low-carb, high-protein (LCHP) diet, emphasizes the consumption of proteins as the primary macronutrient.
This type of eating plan is commonly used to support weight loss, muscle growth, and overall well-being.
The high-protein content can come from a variety of sources, including lean meats, fish, eggs, dairy products, and plant-based options like beans, lentils, and soy.
High-protein diets have been the subject of extensive research, with studies examining their effects on body composition, metabolism, and various health outcomes.
The SWR/J mouse strain and the High Protein Monkey Diet no. 5045 have been utilized in animal studies to investigate the impacts of high-protein diets on factors like muscle growth and body weight regulation.
Similarly, the NZ10 mice and the TD.88137 diet have been employed to explore the metabolic and physiological changes associated with high-protein intake.
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