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Islets of Langerhans Transplantation

Islets of Langerhans Transplantation: Unlocking the Path to Effective Diabetes Treatment.
Islets of Langerhans, the insulin-producing clusters within the pancreas, play a crucial role in regulating blood sugar levels.
Transplantation of these islets has emerged as a promising approach for treating type 1 diabetes, offering the potential to restore normal glycemic control.
PubCompare.ai's AI-driven platform enhances the reproducibility and accuracy of islet transplantation research, empowering researchers to easily locate protocols from literature, pre-prints, and patents, and utilize AI-driven comparisons to identify the best protocols and products for their studies.
Improve your outcomes with PubCompare.ai's intuative tools and take a step closer to revolutionizing diabetes care.

Most cited protocols related to «Islets of Langerhans Transplantation»

Optimized Islet Isolation Protocol

Cited 10 times

In compliance with federal regulations, islet isolations were performed from deceased donors (research/clinical) (n=94), split pancreas digestion (n=5), and chronic pancreatitis (n=150) pancreases. On arrival at the laboratory, the pancreas was trimmed, cannulated, and distended with tissue dissociation enzymes of various combinations. After ductal perfusion of the enzymes, the pancreas was digested using a modified Ricordi’s semi-automated method (31 (link)). The digested tissue was then purified by continuous iodixanol (OptiPrep™, Axis-Shield, Oslo, Norway) density gradient on a COBE-2991 cell processor.
Clinical pancreases were accepted following standard organ acceptance criteria and the Edmonton pancreas donor scoring algorithm was applied to each donor (32 (link)). In clinical allograft isolations, our University of Minnesota isolation protocol (17 (link)) was used for all isolations performed with EC-A (n=13) and EC-F (n=19). For clinical isolations performed with the NEM, 2 were performed with this protocol while the remaining 8 isolations were performed with the Clinical Islet Transplantation (CIT) Consortium islet isolation protocol. In all cases of allotransplantation, the liberated islets were first cultured in CMRL-1066 supplemented medium (Mediatech, Inc, Manassas, VA) for 36–72h before being transplanted.
Autologous islet isolations were performed following total pancreatectomy as described (33 (link)–35 (link)). The isolated islets were transplanted immediately after isolation.
Split pancreas digestions (n=5) were performed on research pancreases to study the potency of the NEM compared to EC-F (Table-1C). Each pancreas was split into two lobes, head/body and body/tail, and each portion was digested with either intact collagenase and ChNP (NEM) or intact collagenase and thermolysin (EC-F). Digestions were performed sequentially and each lobe received alternating enzyme treatments to reduce intra-pancreatic variability.

Balamurugan A.N., Loganathan G., Bellin M.D., Wilhelm J.J., Harmon J., Anazawa T., Soltani S.M., Radosevich D.M., Yuasa T., Tiwari M., Papas K.K., McCarthy R., Sutherland D.E, & Hering B.J. (2012). A new enzyme mixture to increase the yield and transplant rate of autologous and allogeneic human islet products. Transplantation, 93(7), 693-702.

Publication 2012

Allografts Clinical Protocols Collagenase Culture Media Digestion Donors Enzymes Epistropheus Head Hereditary pancreatitis Human Body iodixanol Islets of Langerhans Transplantation isolation Pancreas Pancreatectomy Perfusion Tail Thermolysin Tissues

Mouse Pancreatic Intraductal Virus Infusion

Cited 7 times

All mouse experiments were approved by the Animal Research and Care Committee at the Children’s Hospital of Pittsburgh and the University of Pittsburgh IACUC. BAC transgenic glucagon promoter Cre reporter (GCG-Cre) mice were purchased from MMRRC (Chapel Hill, NC, USA) [30 (link)]. The BAC transgenic elastase promoter CreERT reporter (Ela-CreERT) mouse has been described before [31 (link), 32 (link)]. C57/6, Rosa26CAGTomato (Tomato) and BAC transgenic mouse insulin promoter green fluorescent protein reporter (MIP-GFP) mice [20 (link)] were all purchased from Jackson ImmunoResearch (Bar Harbor, Maine, USA). Tamoxifen induction of Tomato expression in acinar cells in Ela-CreERT; Tomato mice have been described before [32 (link)]. All experiments used 8-week-old male mice. Fasting blood glucose monitoring and intraperitoneal glucose tolerance test (IPGTT) were performed as described previously [20 (link), 33 (link), 34 (link)].
Pancreatic intraductal virus infusion was performed as described previously [32 (link), 35 (link)], but here we delivered 50 μl adeno-associated virus serotype6 vectors (AAV6) (titration of 10⁹) via catheter at a rate of 10 μl/min to optimise infection of duct cells in the current study. Islet transplantation was performed on a heated plate. The left kidney of the mouse was exposed through a lumbar incision. The kidney capsule was then incised with a Hamilton syringe (Fisher Scientific, Pittsburgh, PA, USA) and a pocket was made with polyethylene tubing, after which islets were placed under the kidney capsule. The incision in the kidney capsule was cauterised and the peritoneum and skin were closed with suture.

Xiao X., Prasadan K., Guo P., El-Gohary Y., Fischbach S., Wiersch J., Gaffar I., Shiota C, & Gittes G.K. (2014). Pancreatic duct cells as a source of VEGF in mice. Diabetologia, 57(5), 991-1000.

Publication 2014

Acinar Cell Animals, Transgenic Blood Glucose Capsule Catheters Cells Cloning Vectors Dependovirus Glucagon Glucose Tolerance Test Green Fluorescent Proteins Infection Institutional Animal Care and Use Committees insulin, N(alpha)(B1), biotinyl-epsilon-aminocaproyl- Islets of Langerhans Transplantation Kidney Lumbar Region Lycopersicon esculentum Males Mice, Laboratory Mice, Transgenic Nephrotomy Pancreas Pancreatic Elastase Peritoneum Polyethylene, High-Density Skin Sutures Syringes Tamoxifen Titrimetry Virus

Multicenter Cohort Study of SOT Recipients

Cited 7 times

The STCS is a prospective multicenter cohort which was designed as a dynamic cohort study where SOT recipients move in and out as time progresses [12 ]. We define prospective in the sense that data definitions were made in agreement with the rationale of the study prior to the enrolment period, and that measurements are made in agreement with these definitions [13 ]. A version control strategy has been implemented that ensures data consistency over time, should changes or an updating of the data definitions become necessary.
A patient is considered as transplanted and therefore enrolled at the moment of transplantation, i.e. when the surgeon releases the clamps to start reperfusion of the allograft. For islets transplantation, we defined transplantation as the moment when the islets are injected into the recipient. At the time point of transplantation, the “patient clock” is set to zero initiating prospective follow-up of both the patient and the corresponding allograft(s). Any subsequent transplantation that may occur for a patient is prospectively registered within the patient-case system. Patient follow-up ends with death or definitive drop-out. Non-fatal graft failure does not truncate a patient’s follow-up (e.g. kidney transplant recipients).

Koller M.T., van Delden C., Müller N.J., Baumann P., Lovis C., Marti H.P., Fehr T., Binet I., De Geest S., Bucher H.C., Meylan P., Pascual M, & Steiger J. (2013). Design and methodology of the Swiss Transplant Cohort Study (STCS): a comprehensive prospective nationwide long-term follow-up cohort. European Journal of Epidemiology, 28(4), 347-355.

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Publication 2013

Allografts Grafts Islets of Langerhans Transplantation Kidney Kidney Transplantation Patient Discharge Patients Reperfusion Surgeons Transplantation Transplant Recipients

Isolation and Culture of Rat and Human Islets

Cited 6 times

Rat islets were isolated from pancreases of P5 Sprague Dawley rats (Charles River) as previously described12 (link) except using Liberase TL (Roche) for pancreas digestion. Hand-picked islets were cultured at 37 °C in 10 cm non-adherent cell culture dishes (500 islets/dish) in islet basal medium: RPMI 1640 medium with GlutaMAX (Gibco), 11 mM glucose, 10% fetal bovine serum (FBS), 1% Penicillin/Streptomycin.
Human pancreatic islets were obtained through the European Consortium on Islet Transplantation, (ECIT), Islets for Basic Research Program. Human islets were cultured at 24 °C in 10 cm non-adherent cell culture dishes (500 islets/dish) in CMRL medium with 2% glutamine, 10% FBS, 10 mM HEPES and 1% Penicillin/Streptomycin. Human islets used in this study were obtained from three normal non-diabetic donors, two male and one female, ranging in age 18–59 years with a body mass index of 20–29 kg/m².

Phelps E.A., Cianciaruso C., Santo-Domingo J., Pasquier M., Galliverti G., Piemonti L., Berishvili E., Burri O., Wiederkehr A., Hubbell J.A, & Baekkeskov S. (2017). Advances in pancreatic islet monolayer culture on glass surfaces enable super-resolution microscopy and insights into beta cell ciliogenesis and proliferation. Scientific Reports, 7, 45961.

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Publication 2017

Cell Culture Techniques Digestion Donors Europeans Females Fetal Bovine Serum Glucose Glutamine HEPES Homo sapiens Hyperostosis, Diffuse Idiopathic Skeletal Index, Body Mass Islets of Langerhans Islets of Langerhans Transplantation Liberase Males Pancreas Penicillins Rats, Sprague-Dawley Rivers Streptomycin

Islet Transplantation for Metabolic Control

Cited 6 times

Islet transplantation consisted of up to three sequential fresh islet infusions within 3 months, with the aim of reaching adequate metabolic control without exogenous insulin. Islets were isolated from ABO-compatible deceased donor pancreata with a negative cross-match and evaluated as previously described (8 (link)). The access to the portal vein was gained under general anesthesia by percutaneous catheterization of a peripheral portal branch under ultrasound guidance or by surgical catheterization of a small mesenteric vein. In all cases, heparin (35 units/kg) was added to the final product, gently infused by gravity with portal pressure monitoring. Immunosupression consisted of tacrolimus (Prograf) (Astellas, Fujisawa, Japan), target trough levels at 3–6 ng/ml, and sirolimus (Rapamune) (Wyeth Pharmaceuticals France, Paris, France), target trough levels at 12–15 ng/ml for 3 months and at 7–10 ng/ml thereafter. A five-dose induction course of dacluzimab (Zenapax) (1 mg/kg) (Roche, Welwyn Garden City, U.K.) was administered biweekly beginning 1 h before the first infusion.

Vantyghem M.C., Kerr-Conte J., Arnalsteen L., Sergent G., Defrance F., Gmyr V., Declerck N., Raverdy V., Vandewalle B., Pigny P., Noel C, & Pattou F. (2009). Primary Graft Function, Metabolic Control, and Graft Survival After Islet Transplantation. Diabetes Care, 32(8), 1473-1478.

Publication 2009

Catheterization Catheterization, Peripheral Crossmatching, Blood Donors General Anesthesia Gravity Heparin Insulin Islets of Langerhans Transplantation Operative Surgical Procedures Pancreas Pharmaceutical Preparations Portal Pressure Prograf Rapamune Sirolimus Tacrolimus Ultrasonic Shockwave Vein, Mesenteric Veins, Portal Zenapax

Most recents protocols related to «Islets of Langerhans Transplantation»

hPIs Encapsulation and Culturing

Cell cultures were prepared within 24/48 h after receiving islets. hPIs were incapsulated inside hydrogels or plated in suspension in 48-well with two different type of culture media: MIAMI medium #1A (Mediatech-Cellgro, VA, United States) (a CMRL-based culture medium commonly used in clinical trial for islet culture before transplantation) or GF medium (a serum-free medium with bFGF and EGF growth factors). 25 IEQ (low density) or 500 IEQ (high density) were incapsulated inside SAPs, previously dissolved in distilled water and diluted with 584 mM sucrose solution (ratio 1:1). A droplet of 25 µL was placed onto glass coverslip in 48-well, medium was added to start SAP gelation and to obtain free-floating samples. Same concentration of islets was used for samples in suspension in 48-well. In these conditions, the samples were maintained in culture up to 14-days (T14) and 28-days (T28) at 24°C, 20% O₂, 5% CO₂ in a humidified atmosphere. As positive control, suspensions of islets in MIAMI medium or GF medium were maintained in culture for 1 day (T1). hPIs incapsulated insides hydrogel were monitored individually during culture time and brightfield images from day 1 to day 28 were acquired weekly via Zeiss light microscope at ×5 magnification.

Marchini A., Ciulla M.G., Antonioli B., Agnoli A., Bovio U., Visnoviz V., Bertuzzi F, & Gelain F. (2023). Long-term cultures of human pancreatic islets in self-assembling peptides hydrogels. Frontiers in Bioengineering and Biotechnology, 11, 1105157.

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Publication 2023

Atmosphere Cell Culture Techniques Growth Factor Hydrogels Islets of Langerhans Transplantation Light Microscopy Serum SKAP2 protein, human Sucrose

Batch Allocation Algorithm for Gene Expression Analysis

We illustrate our novel batch allocation algorithm (Additional file 1) in a hypothetical case–control study. In order to create a biologically plausible scenario, we downloaded a publicly available microarray gene expression dataset from NCBI GEO, GSE50397. The dataset includes gene expression data from 89 human pancreas islet donors. The samples were obtained from Nordic Islet Transplantation Programme, Uppsala University, see http://www.nordicislets.org for more information about islet processing and isolation. Data processing is described in greater detail in previous publications [27 (link)–29 (link)]. In brief, microarray profiling was performed using the Affymetrix GeneChip^® Human Gene 1.0 ST whole transcript platform. Using the oligo R package, the Robust Multi-array Analysis (RMA) method was used to summarize and normalize the array data. Batch correction was performed using COMBAT function from SVA package [7 (link)]. The top 10,000 most variable genes from the batch corrected dataset (downloaded from GEO) were used for all subsequent analyses as the ‘true’ gene expression values.

Carry P.M., Vigers T., Vanderlinden L.A., Keeter C., Dong F., Buckner T., Litkowski E., Yang I., Norris J.M, & Kechris K. (2023). Propensity scores as a novel method to guide sample allocation and minimize batch effects during the design of high throughput experiments. BMC Bioinformatics, 24, 86.

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Publication 2023

Donors Gene Chips Gene Expression Genes Homo sapiens Islets of Langerhans Islets of Langerhans Transplantation isolation Microarray Analysis Oligonucleotides

Ethical Human Tissue Acquisition

The study has been conducted in accordance with the Declaration of Helsinki as well as the local and national guidelines. Anonymized human blood samples were obtained from the Uppsala University Hospital blood bank. Human islets were obtained from non-diabetic deceased donors within the Nordic Network for Clinical Islet Transplantation Laboratory (Uppsala University Hospital, Uppsala Sweden) through isolation methods described by Goto et al. [15 (link)].
All organ donors in Sweden provided written consent that their donated tissues may be entered into a biobank and used in medical research, following review and approval by the Regional Ethics Board (now Swedish Ethical Review Authority) of individual studies. The obtained tissues were anonymized, collected and treated according to local institutional and Swedish national rules and regulations, with the need for informed consent renounced by the Regional Ethics Board in Uppsala.
The use of human tissues from Uppsala Biobank (registration #827) was approved by the Regional Ethics Board, Uppsala, Sweden (2011/473, Ups 02-577, 2015/401).

Cheung P., Amin M.A., Zhang B., Lechi F., Korsgren O., Eriksson J., Odell L.R, & Eriksson O. (2023). [18F]MK-7246 for Positron Emission Tomography Imaging of the Beta-Cell Surface Marker GPR44. Pharmaceutics, 15(2), 499.

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Publication 2023

BLOOD Clinical Laboratory Services Donor, Organ Donors Ethical Review Homo sapiens Islets of Langerhans Transplantation isolation Tissues

Isolation and Culture of Human and Mouse Pancreatic Islets

Pancreatic islets were obtained from human cadaveric donors by the Nordic Network for Clinical Islet Transplantation (ethical approval by Uppsala Regional Ethics Board 2006/348) [32 (link)] or the ADI Isletcore at the University of Alberta (ethical approval by Alberta Human Research Ethics Board, Pro00001754) [33 (link)], with written donor and family consent for use in research. Work with human tissue complied with all relevant ethical regulations for use in research and the study was approved by the Gothenburg Regional Ethics Board, Sweden and ethical committees at Indian Institute of Science, India. Isolated islets were cultured in free-floating sterile dishes in RPMI 1640 culture medium containing 5.5 mmol/L glucose, 10% fetal bovine serum (FBS), streptomycin (100 U/mL), and penicillin (100 U/mL) at 37 °C in an atmosphere of 5% CO₂ up to a week.
Mouse islets were isolated from 13 to 17-week-old WT, ChR2^+/−-SST^+/−iCre or ChR2^+/−-Glu^+/−Cre. Mice were anesthetized and sacrificed by cervical dislocation. Canulation of the bile duct was performed with a 30G needle and a Liberase solution (Liberase TL Roche TM) was injected in the pancreas. The pancreas was excised and digested at 37 °C for 10–12 min. The islets were handpicked under a stereo microscope in Hanks’ balanced salts buffer (HBS) supplemented with 0.1% BSA and 5 mmol/L glucose. Isolated islets were cultured in RPMI 1640 culture medium containing 5.5 mmol/L glucose, 10% fetal bovine serum (FBS), streptomycin (100 U/mL), and penicillin (100 U/mL) at 37 °C in an atmosphere of 5% CO₂.
All animal experiments were previously approved by the ethics committee at the Sahlgrenska Academy, Gothenburg University, Sweden (approval number: 948/17) and Institutional Animal Ethics Committee (IHEC), Indian Institute of Science, India (approval number: CAF/Ethics/880/2022) respectively.

Kothegala L., Miranda C., Singh M., Krieger J.P, & Gandasi N.R. (2023). Somatostatin Containing δ-Cell Number Is Reduced in Type-2 Diabetes. International Journal of Molecular Sciences, 24(4), 3449.

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Publication 2023

Animals Atmosphere Buffers Cannulation Culture Media Donors Duct, Bile Ethics Committees Fetal Bovine Serum Glucose Homo sapiens Hyperostosis, Diffuse Idiopathic Skeletal Institutional Ethics Committees Islets of Langerhans Islets of Langerhans Transplantation Joint Dislocations Liberase Mice, House Microscopy Neck Needles Pancreas Penicillins Salts Sterility, Reproductive Streptomycin Tissue Donors Tissues

Engrafting Human Islets in Scid-Beige Mice

The immunodeficient scid-beige mice (Model#CBSCBG-M; C.B-Igh-1b/GbmsTac-Prkdc^scid-Lyst^bg N7) used as a recipient of human islet transplant were purchased from Taconic (Rensselaer, NY, United States), and were housed in a pathogen free facility before and after human islet transplantation. Islet transplantation was performed as described elsewhere (Pagliuca et al., 2014 (link)). 10–17 weeks old mice received 1,500–2,000 IEQ per mouse. 4–8 weeks later, blood was collected to confirm the presence of human insulin in the blood stream using human insulin ELISA (80-INSHU-E01, ALPCO, Salem, NH, United States) before initiation of treatment with ASO. Successful engraftment of human islets was affirmed by the presence of plasma human insulin in all 12 transplanted mice but not in non-transplanted controls. Fasting plasma insulin ranged from 4.7 to 31.0 μIU/ml, sensitivity of the assay 0.135 μIU/ml.

Gurlo T., Prakash T.P., Wang Z., Archang M., Pei L., Rosenberger M., Pirie E., Lee R.G, & Butler P.C. (2023). Efficacy of IAPP suppression in mouse and human islets by GLP-1 analogue conjugated antisense oligonucleotide. Frontiers in Molecular Biosciences, 10, 1096286.

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Publication 2023

Biological Assay BLOOD Blood Circulation Enzyme-Linked Immunosorbent Assay Homo sapiens Hypersensitivity Immunologic Deficiency Syndromes Insulin Islets of Langerhans Transplantation Mice, Laboratory pathogenesis Plasma SCID Mice Transplant Recipients

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More about "Islets of Langerhans Transplantation"

Islets of Langerhans are specialized clusters of cells within the pancreas that play a crucial role in regulating blood sugar levels by producing the hormone insulin.
Transplantation of these insulin-secreting islet cells has emerged as a promising approach for treating type 1 diabetes, offering the potential to restore normal glycemic control and reduce or eliminate the need for exogenous insulin administration.
The process of islet transplantation typically involves isolating the islet cells from a donor pancreas and then transplanting them into the recipient, often within the liver.
This procedure requires the use of various culture media and reagents, such as STZ (streptozotocin) for pancreatic islet isolation, CMRL 1066 medium for islet culture, and supplements like FBS (fetal bovine serum), penicillin, streptomycin, gentamicin, ciprofloxacin, and Fungizone to support islet viability and function.
PubCompare.ai's AI-driven platform enhances the reproducibility and accuracy of islet transplantation research by enabling researchers to easily locate and compare protocols from the literature, preprints, and patents.
This empowers researchers to identify the most effective protocols and products for their studies, ultimately improving the outcomes and advancing the field of diabetes treatment.
The TruSeq RNA Sample Preparation Kit is a commonly used tool in islet transplantation research, allowing for the extraction and purification of high-quality RNA from islet cells for downstream analyses, such as gene expression profiling and transcriptomics.
By leveraging these advanced tools and technologies, researchers can gain deeper insights into the molecular mechanisms underlying islet function and the impact of transplantation on glucose homeostasis.
Overall, the field of islets of Langerhans transplantation holds great promise for revolutionizing the management of type 1 diabetes, and the innovative solutions offered by PubCompare.ai are poised to accelerate progress in this critical area of biomedical research.