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> Procedures > Therapeutic or Preventive Procedure > Microsurgery

Microsurgery

Microsurgery is a specialized field of surgery that involves the use of microscopes and other magnification devices to perform delicate procedures on small structures, such as blood vessels, nerves, and tissue.
This precision-based approach allows for intricate repairs and reconstructions, often in the context of plastic and reconstructive surgery, neurosurgery, and other medical disciplines.
By leveraging advanced techniques and technologies, microsurgeons are able to restore function and improve outcomes for patients with complex medical conditions.
The latest innovations in AI-driven protocol optimizatioin are revolutionizing the field, enabling researchers to identify the most effective treatments and drive breakthroughs in microsurgery.

Most cited protocols related to «Microsurgery»

1
Zebrafish Embryo Microsurgery and Imaging
Cited 19 times
Zebrafish and their embryos were handled according to standard protocols23 and in accordance with University of Massachusetts Medical School IACUC guidelines. For laser-assisted microsurgery, embryos at 46 hpf were anesthetized and immobilized in 0.5% of low-melt agarose (Biorad). The connection between AA5 and AA6 and the ventral aorta was ablated using a Micropoint laser (Photonic Instrument, Inc) mounted on a Zeiss AX10 Imager M1. SU5416 (Calbiochem) was prepared and used as described previously11 (link). Control embryos were treated with 0.1% dimethyl sulfoxide (DMSO). To arrest heartbeat, embryos were treated with 15 mM of 2,3-butanedione 2-monoxime (BDM; Sigma-Aldrich) or with buffered Tricaine methanesulfonate (Sigma-Aldrich) at 0.66 mg/ml in egg water for the indicated times. Two-photon time-lapse imaging, confocal microscopy and microangiography was performed as previously13 (link), 24 (link), with additional modifications as noted in Supplementary Methods. Antisense riboprobes against dll4, vegfa, kdrl, fli1a, and cdh5 were generated and used for whole mount in situ hybridization as described elsewhere25 (link). A klf2a fragment was PCR amplified and cloned by Gateway recombination. The resulting clone was linearized with BglII and a DIG-labeled riboprobe was synthesized using T7 polymerase. Digoxigenin (DIG)-labeled locked nucleic acid (LNA) probes (Exiqon, Copenhagen) were used to detect mature miR-126 and let-7 using in situ hybridization or Northern analysis as described elsewhere18 (link). Morpholinos, mRNA and Tol2-based plasmids were prepared and injected as previously11 (link),21 (link). In cases of co-injection with Morpholinos, Tol2-plasmids and transposase, a DNA/transposase mRNA mixture was initially injected, followed by Morpholino. Plasmid construction details are provided in the full methods section. Morpholinos against vegfa, tnnt2 and gata1 have been described elsewhere15 (link), 26 (link), 25 (link); all other Morpholino and oligonucleotide sequences are provided in the full methods section.
Nicoli S., Standley C., Walker P., Hurlstone A., Fogarty K.E, & Lawson N.D. (2010). microRNA-mediated integration of haemodynamics and Vegf signaling during angiogenesis. Nature, 464(7292), 1196-1200.
Publication 2010
Aorta Cardiac Arrest CDH5 protein, human Clone Cells diacetylmonoxime Digoxigenin DNA, A-Form Embryo GATA1 protein, human In Situ Hybridization Institutional Animal Care and Use Committees locked nucleic acid methanesulfonate Microscopy, Confocal Microsurgery Morpholinos Nucleic Acid Probes Oligonucleotides Plasmids Pulse Rate Recombination, Genetic RNA, Messenger Sepharose SU 5416 Sulfoxide, Dimethyl Transposase tricaine Zebrafish
2
Zebrafish Embryo Microsurgery and Imaging
Cited 19 times
Zebrafish and their embryos were handled according to standard protocols23 and in accordance with University of Massachusetts Medical School IACUC guidelines. For laser-assisted microsurgery, embryos at 46 hpf were anesthetized and immobilized in 0.5% of low-melt agarose (Biorad). The connection between AA5 and AA6 and the ventral aorta was ablated using a Micropoint laser (Photonic Instrument, Inc) mounted on a Zeiss AX10 Imager M1. SU5416 (Calbiochem) was prepared and used as described previously11 (link). Control embryos were treated with 0.1% dimethyl sulfoxide (DMSO). To arrest heartbeat, embryos were treated with 15 mM of 2,3-butanedione 2-monoxime (BDM; Sigma-Aldrich) or with buffered Tricaine methanesulfonate (Sigma-Aldrich) at 0.66 mg/ml in egg water for the indicated times. Two-photon time-lapse imaging, confocal microscopy and microangiography was performed as previously13 (link), 24 (link), with additional modifications as noted in Supplementary Methods. Antisense riboprobes against dll4, vegfa, kdrl, fli1a, and cdh5 were generated and used for whole mount in situ hybridization as described elsewhere25 (link). A klf2a fragment was PCR amplified and cloned by Gateway recombination. The resulting clone was linearized with BglII and a DIG-labeled riboprobe was synthesized using T7 polymerase. Digoxigenin (DIG)-labeled locked nucleic acid (LNA) probes (Exiqon, Copenhagen) were used to detect mature miR-126 and let-7 using in situ hybridization or Northern analysis as described elsewhere18 (link). Morpholinos, mRNA and Tol2-based plasmids were prepared and injected as previously11 (link),21 (link). In cases of co-injection with Morpholinos, Tol2-plasmids and transposase, a DNA/transposase mRNA mixture was initially injected, followed by Morpholino. Plasmid construction details are provided in the full methods section. Morpholinos against vegfa, tnnt2 and gata1 have been described elsewhere15 (link), 26 (link), 25 (link); all other Morpholino and oligonucleotide sequences are provided in the full methods section.
Nicoli S., Standley C., Walker P., Hurlstone A., Fogarty K.E, & Lawson N.D. (2010). microRNA-mediated integration of haemodynamics and Vegf signaling during angiogenesis. Nature, 464(7292), 1196-1200.
Publication 2010
Aorta Cardiac Arrest CDH5 protein, human Clone Cells diacetylmonoxime Digoxigenin DNA, A-Form Embryo GATA1 protein, human In Situ Hybridization Institutional Animal Care and Use Committees locked nucleic acid methanesulfonate Microscopy, Confocal Microsurgery Morpholinos Nucleic Acid Probes Oligonucleotides Plasmids Pulse Rate Recombination, Genetic RNA, Messenger Sepharose SU 5416 Sulfoxide, Dimethyl Transposase tricaine Zebrafish
3
Quantifying Egg-Laying Behavior in C. elegans
Cited 11 times
Transgenic lines were scored as egg-laying defective if young adults (24 h after late L4) had, on average, greater than 30 eggs in utero. To score the developmental stages of newly laid eggs, young adults were transferred to fresh nematode growth medium plates with bacteria, five worms per plate, for 1 h at room temperature and then removed. Operated worms were assayed individually, and a single operated worm was assayed two and four times.
Eggs on the plate were examined under a high-power dissecting microscope and categorized as described in Figure 2.
Distributions of the developmental stages of eggs laid by worms of different genotypes were analyzed with the Wilcoxon Mann-Whitney rank-sum test, a nonparametric test of statistical significance, as implemented in the Coin package of the R statistical analysis program49 . The results of statistical tests of significance can be found in Supplementary Table 1. Egg-laying defects of many strains were also independently quantified by measuring the number of retained eggs in staged adults as previously described19 (link), and we observed similar interactions between receptor- and ligand-encoding genes, receptor- and G protein-encoding genes, and genes in the egl-6 signaling pathway and acetylcholine biosynthesis genes (data not shown).
Laser microsurgeries were performed on L2-stage larvae as described50 (link). Cell identifications were made on the basis of nuclear position and cell morphology. A gcy-33∷gfp transgene was used in some experiments to identify BAG neurons (see Supplementary Methods).
Ringstad N, & Horvitz H.R. (2008). FMRFamide neuropeptides and acetylcholine synergistically inhibit egg-laying by C. elegans. Nature neuroscience, 11(10), 1168-1176.
Publication 2008
Acetylcholine Adult Anabolism Animals, Transgenic Bacteria Cells Culture Media Eggs Gene Products, Protein Genes Genotype Helminths Larva Ligands Microscopy Microsurgery Nematoda Neurons Signal Transduction Strains Transgenes Uterus Young Adult
4
Isolation of Murine Synovial Tissue
Cited 5 times
Mice were sacrificed by cervical dislocation and immersed in 75% alcohol for 2 min for sterilization. Under a stereomicroscope (Olympus, Tokyo, Japan), the skin of the hind limbs was removed and the synovial tissues around the hip joints were obtained using microsurgery scissors and forceps (white sponge; Fig. 1A). During this procedure, attention was focused on eliminating the ‘egg-yolk’-like yellow oval substance (insert in Fig. 1B). The synovium is transferred to a 60-mm Petri dish containing 2 ml of DMEM.
To obtain greater amounts of synovial tissues, the following procedures were implemented: Isolated the hind limbs (preserved all the muscle tissues and discarded foot and ankles) and placed in a 60-mm Petri dish containing 2 ml of DMEM. Under a stereomicroscope, the muscle inside the popliteal fossa was cut open with microsurgery scissors and forceps to harvest the synovium (insert 2 in Fig. 1C). During this procedure, attention was focused on eliminating the ‘egg-yolk’-like yellow oval substance (insert in Fig. 1D) in the middle of the synovium. Subsequently, the synovium was transferred to another 60-mm Petri dish containing 2 ml of DMEM. To harvest the intra-articular synovium, the articular cavity of the knee was cut open along both sides of the patella under a stereomicroscope (insert 3 in Fig. 1C), and isolated the intra-articular synovium carefully. Of note, the connective tissues around the synovium were carefully eliminated under a stereomicroscope.
Zhao J., Ouyang Q., Hu Z., Huang Q., Wu J., Wang R, & Yang M. (2016). A protocol for the culture and isolation of murine synovial fibroblasts. Biomedical Reports, 5(2), 171-175.
Publication 2016
Ankle Attention Connective Tissue Dental Caries Ethanol Foot Forceps Hip Joint Hyperostosis, Diffuse Idiopathic Skeletal Joint Dislocations Joints Knee Joint Mice, House Microsurgery Muscle Tissue Neck Patella Porifera Skin Sterilization, Reproductive Synovial Membrane Yolks, Egg
5
Laser Microsurgery of Caenorhabditis elegans Neurons
Cited 5 times
An amplified Titanium:sapphire laser system emits a 1-kHz train of near infrared pulses (λ = 800 nm), with pulse durations of about 100 fs. By serial insertion of neutral density filters and Kepler telescopic lenses, we adjusted the pulse energy to about 3 nJ at the sample and overfilled the back-aperture of a Zeiss Plan Apochromat 63x, 1.4-NA oil-immersion objective. The same objective was used to image the sample by epifluorescence microscopy using a Coolsnap CCD camera (Roper Scientific Photometrics, Tucson, AZ). The image plane of the CCD camera was adjusted to the laser focus by visualizing the plasma emission created by higher energy laser pulses in coverslip glass.
For femtosecond laser ablation, we followed pre – and postoperative procedures established for standard laser microsurgery [24 (link)]. We severed AFD dendrites in transgenic L4 or young adult worms that had been cultivated overnight at 20°C. We killed AFD neurons in L1 worms. We killed AIZ neurons in L2 worms, the larval stage at which the fluorescent label of AIZ became unambiguous. Worms were rinsed in NGM buffer, anaesthetized with 0.2–0.8 mM sodium azide, and mounted on a thin pad of 2% agarose between a glass coverslip and slide. Using fluorescence microscopy and a three-axis piezoelectric nanopositioning stage (Thorlabs MDT630), the target was placed in the focal point of laser pulses and then ablated. Worms were recovered from the sodium azide treatment within 30–60 minutes by rinsing them in NGM buffer for 20 minutes and then placing them on fresh plates with bacterial food. After recovery from sodium azide, worms were immediately returned to the 20°C incubator for at least 12 h before undergoing behavioral assays as young adults.
Chung S.H., Clark D.A., Gabel C.V., Mazur E, & Samuel A.D. (2006). The role of the AFD neuron in C. elegans thermotaxis analyzed using femtosecond laser ablation. BMC Neuroscience, 7, 30.
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Publication 2006
Animals, Transgenic Bacteria Biological Assay Buffers Dendrites Enzyme Multiplied Immunoassay Technique Epistropheus Food Helminths Larva Laser Ablation Lens, Crystalline Microscopy Microscopy, Fluorescence Microsurgery Neurons Plasma Postoperative Procedures Pulse Rate Sapphire Sepharose Sodium Azide Strains Submersion Telescopes Titanium Young Adult

Most recents protocols related to «Microsurgery»

1
Microsurgical Skills of Plastic Surgery Fellows
This study was conducted to assess the microsurgical skills and surgical outcomes of microsurgical fellows who completed either an independent or integrated plastic surgery residency and underwent a 1-year microsurgery fellowship at The University of Texas MD Anderson Cancer Center from March 2005 to June 2019. This study was approved by the MD Anderson institutional review board and followed the Strengthening the Reporting of Observational Studies in Epidemiology guidelines.
Hassan A.M., Egro F.M., Talanker M.M., Shah N.R., Liu J., Maricevich R.S., Chang E.I., Hanasono M.M., Selber J.C, & Butler C.E. (2023). Comparison of Long-term Surgical Outcomes and Microsurgical Skills between Independent and Integrated Plastic Surgery Trainees. Plastic and Reconstructive Surgery Global Open, 11(3), e4709.
Publication 2023
Ethics Committees, Research Fellowships Malignant Neoplasms Microsurgery Operative Surgical Procedures Plastic Surgical Procedures Residency
2
Bilateral Ovariectomy in Rodents
Animals were anesthetized by intraperitoneal administration of 2.5% solution of 2,2,2-tribromethanol (Sigma-Aldrich, St. Louis, MO, USA) at a dose of 400 mg/kg and bilateral ovariectomy was performed under aseptic conditions using microsurgery technique. The animals of the same age which had only incisions of the abdominal cavity and isolation of the ovaries without resection (sham-operated) were used as a control group. Wounds were sutured in layers; the animals were kept under a heat lamp until the recovery from anesthesia.
Kyryk V., Tsupykov O., Ustymenko A., Smozhanik E., Govbakh I., Butenko G, & Skibo G. (2023). Age-related ultrastructural changes in spheroids of the adipose-derived multipotent mesenchymal stromal cells from ovariectomized mice. Frontiers in Cellular Neuroscience, 17, 1072750.
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Publication 2023
Abdominal Cavity Anesthesia Animals Asepsis Female Castrations Injections, Intraperitoneal isolation Microsurgery Ovary Wounds
3
Microsurgical Treatment of Azoospermia
The study included 24 patients, representing the first treatment experience using micro-surgical methods in patients with azoospermia in the Republic of Moldova. Patients underwent investigations according to the pre-established algorithm: sperm evaluation accordingly to the WHO 2010 guide recommendations, hormonal testing (LH, FSH, testosterone, SHBG, prolactin, and estradiol), ultrasound of the scrotum and relevant genetic examination (karyotype, AZF microdeletions, and CFTR mutations) before surgical treatment. Azoospermia was established when the absence of spermatozoa was confirmed in two semen samples. Thus, according to WHO guidelines, sediment analysis was performed after 600 g centrifugation for 15 minutes and screening at 400x magnification using an inverted microscope. The same team worked on all interventions and post-intervention tissue analyses. The tissue removed during microsurgery was carefully examined using a combination of mechanical and enzymatic maceration techniques. Samples were preserved by freezing and then subjected to both histological and immunohistochemical testing to obtain detailed information about the tissue.
Arian I., Dumbraveanu I., Ghenciu V., Machidon D., Ernu I, & Ceban E. (2023). Histological and immunohistochemical outcomes after microdissection TESE in contrast with hormonal profile, testis volume and genetics in patients with azoospermia. Journal of Medicine and Life, 16(1), 144-152.
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Publication 2023
Azoospermia Centrifugation Cystic Fibrosis Transmembrane Conductance Regulator Enzymes Estradiol Genetic Testing Karyotyping Microscopy Microsurgery Mutation Operative Surgical Procedures Patients Plant Embryos Prolactin Scrotum Sperm Testosterone Tissues Ultrasonics
4
Orthopedic Nursing Workforce in Key Hospital
The Department of Orthopedics of our hospital is a national key construction discipline, integrating trauma, joint, and spine surgeries, bone oncology, microsurgery, sports medicine, and other specialized disease treatment disciplines. Seventy-seven orthopedic nurses were included as the study participants, with the age ranging from 22 to 42 (29.69, 5.34) years; title: one deputy chief nurse (1.30%), 13 chief nurses (16.88%), 51 nurse practitioner (66.23%), and 12 nurses (15.58%); working years: 29 (37.66%) with <5 years, 30 (38.96%) with 5– 10 years, seven (9.09%) with 11– 15 years, and 11 (14.29%) with >15 years; and posts: 19 nursing team leaders or specialized nurses (24.68%), 22 senior nurses (28.57%), and 36 junior nurses (46.75%).
The following are the inclusion criteria: (i) clinical practical nurses registered with the Provincial Health Commission, (ii) clinical practical nurses registered with the Provincial Health Commission, (iii) clinical practical nurses registered with the Provincial Health Commission, with at least orthopaedic nursing work (1 year). The following are the exclusion criteria: (i) nursing managers (head nurses), nurses without a nursing practice certificate were excluded; (ii) working in orthopaedics for <1 year, with long-term leave; and (iii) sick leave, maternity leave; or studying abroad for more than 15 days (nurse).
Zhang W., Huang X, & Huang T. (2023). Individualized Management of Quality of Care in Orthopedic Nurses Based on Sensitive Indicators. Computational and Mathematical Methods in Medicine, 2023, 1950220.
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Publication 2023
Bones Joints Microsurgery Neoplasms Nurse Managers Nurses Nurses, Head Nursing, Team Operative Surgical Procedures Orthopedic Surgical Procedures Practitioner, Nurse Vertebral Column Wounds and Injuries
5
Microsurgical Eye Resection Technique
Eye resection was performed as previously described [62 (link)]. In each experiment, six animals were used in each group. Animals were placed on moist filter paper on a cold block in order to limit movement, while adjusting the focus and magnification of the dissector to make the eyes clearly visible. A microsurgery blade was used to remove the eyes through a small longitudinal dorsal incision.
Wang S., Sun Y., Liu X., Guo Y., Huang Y., Zhang S, & Tian Q. (2023). Meis1 Controls the Differentiation of Eye Progenitor Cells and the Formation of Posterior Poles during Planarian Regeneration. International Journal of Molecular Sciences, 24(4), 3505.
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Publication 2023
Animals Common Cold Eye Microsurgery Movement

Top products related to «Microsurgery»

1
Ultrapure low melting point agarose by Thermo Fisher Scientific
Sourced in United States, United Kingdom
UltraPure Low Melting Point Agarose is a highly purified agarose product used for the preparation of low-melting point gels. It is designed for applications where a low gelling temperature is required, such as cell embedding and in vitro transcription/translation.
2
Opmi pico by Zeiss
Sourced in Germany
The OPMI pico is a microscope system designed for ophthalmic procedures. It features high-quality optics and a compact, ergonomic design. The OPMI pico is engineered to provide a clear and detailed visual field for the user.
3
Rompun by Bayer
Sourced in Germany, France, United States, United Kingdom, Canada, Italy, Brazil, Belgium, Cameroon, Switzerland, Spain, Australia, Ireland, Sweden, Portugal, Netherlands, Austria, Denmark, New Zealand
Rompun is a veterinary drug used as a sedative and analgesic for animals. It contains the active ingredient xylazine hydrochloride. Rompun is designed to induce a state of sedation and pain relief in animals during medical procedures or transportation.
4
C57bl 6j mice by Jackson ImmunoResearch
Sourced in United States, Montenegro, Japan, Canada, United Kingdom, Germany, Macao, Switzerland, China
C57BL/6J mice are a widely used inbred mouse strain. They are a commonly used model organism in biomedical research.
5
Mivacurium chloride by Abcam
Sourced in United Kingdom
Mivacurium chloride is a short-acting neuromuscular blocking agent used in anesthesia and critical care settings. It acts by competitively inhibiting the action of acetylcholine at the neuromuscular junction, resulting in muscle relaxation. Mivacurium chloride is primarily used to facilitate endotracheal intubation and provide muscle relaxation during surgical procedures.
6
Low melting point agarose by Leica
Low melting point agarose is a type of agarose, a polysaccharide derived from red algae, that has a lower melting point compared to standard agarose. It is commonly used in gel electrophoresis and other laboratory applications where a lower melting point is desirable.
7
U shaped glass capillary by Leica
The U-shaped glass capillary is a laboratory equipment component used to precisely measure and transfer small volumes of liquids. It features a U-shaped design that allows for accurate manipulation and delivery of sample materials. The capillary structure enables controlled fluid movement and precise dispensing for various experimental and analytical applications.
8
Hank s buffered saline solution by Thermo Fisher Scientific
Sourced in United Kingdom
Hank's Buffered Saline Solution is a balanced salt solution commonly used in cell culture and laboratory applications. It maintains the osmotic balance and pH of biological samples. The solution contains a mixture of inorganic salts and is buffered to maintain a physiologically relevant pH.
9
Ilaspulse laser system by Teledyne
Sourced in France
The ILasPulse laser system is a compact and powerful device designed for laboratory use. It generates high-energy laser pulses with a precise and adjustable output. The core function of the ILasPulse is to provide researchers and scientists with a reliable and versatile laser source for their experimental needs.
10
Fluid cell lite coverslip holder by Oxford Instruments
Sourced in United States
The Fluid Cell Lite coverslip holder is a laboratory equipment designed to securely hold coverslips in place during microscopy experiments. It provides a stable platform for sample observation and analysis.

More about "Microsurgery"

Microsurgery is a highly specialized field of surgery that leverages advanced techniques and technologies to perform intricate procedures on small structures, such as blood vessels, nerves, and delicate tissues.
This precision-based approach, often employed in plastic and reconstructive surgery, neurosurgery, and other medical disciplines, allows surgeons to restore function and improve outcomes for patients with complex medical conditions.
Key innovations in microsurgery include the use of microscopes and other magnification devices, as well as the latest advancements in AI-driven protocol optimization.
These cutting-edge tools enable researchers to identify the most effective treatments and drive breakthroughs in the field.
For example, microsurgeons may utilize specialized equipment like the OPMI pico microscope, U-shaped glass capillaries, and Hank's buffered saline solution to perform delicate procedures.
Low melting point agarose and Rompun can also be employed in microsurgery research, often involving the use of C57BL/6J mice.
Additionally, innovative laser systems like the ILasPulse laser can be leveraged to enhance precision and outcomes.
The Fluid Cell Lite coverslip holder is another useful tool that can help streamline microsurgical workflows.
By harnessing the power of these advanced technologies and techniques, microsurgeons are able to achieve remarkable results, restoring function and improving the quality of life for patients.
The field of microsurgery continues to evolve, with AI-driven protocol optimization playing a crucial role in driving the next generation of breakthroughs.