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Passive Immunization

Passive Immunization refers to the transfer of active immunoglobulin or antigen-specific T-cells from an immune individual to a non-immune one, providing immediate, short-term protection against a particular pathogen or toxin.
This approach can be used to prevent or treat infectious diseases, autoimmune disorders, and other conditions.
PubCompare.ai's innovative AI-driven platform helps researchers easily identify the most promising passive immunization strategies by locating the best research protocols from literature, pre-prints, and patents using smart comparisons.
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Most cited protocols related to «Passive Immunization»

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Publication 2013
Biotin Buffers Cells Deoxyribonucleases DNA Chips Exons Flow Cytometry Gene Chips Homo sapiens isolation Microscopy Mouse Embryonic Stem Cells Mus neuro-oncological ventral antigen 2, human Passive Immunization Serum Tissues Treatment Protocols

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Publication 2015
alpha HML-1 Antibodies Bicarbonates BLOOD Buffers CD44 protein, human Cells Cervix Uteri Collagenase, Clostridium histolyticum Dithioerythritol Enzymes Female Reproductive System Flow Cytometry Hemoglobin, Sickle HEPES Hyperostosis, Diffuse Idiopathic Skeletal Intestines Intestines, Small isolation Kidney Lamina Propria Large Intestine Liver Lung Lymphocyte Matrix Metalloproteinase 2 Mucus Mus Needles Nodes, Lymph Nylons Pancreas Passive Immunization Percoll Polystyrenes Salivary Glands Spleen Stomach Streptavidin Syringes Thymus Plant Tissues Uterine Cornua Vagina

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Publication 2015
Acetic Acid Buffers Capillaries Electrolytes formic acid Immunoglobulins Methanol Passive Immunization Pressure Proteins Radionuclide Imaging

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Publication 2014
Antibodies Biological Assay Enzyme-Linked Immunosorbent Assay Gammagard GP 140 Grafts HIV-1 HIV-2 Homo sapiens Infant, Newborn Infection Intravenous Immunoglobulins Mus Passive Immunization Serum Stem Cells, Hematopoietic
Neutralizing goat antibody (10 μg) against mouse flt-1 (R&D Systems), isotype control goat IgG (10 μg; Jackson Immunoresearch), shRNAs (4 μg) against mbflt-1 or sflt-1, psflt-1 (4 μg), psflt-1* (4 μg), pCre (4 μg; gift of R.K. Nordeen, University of Colorado), pNull (4 μg), rmVEGF-A164 (20–500 ng; R&D Systems), sflt-1/Fc (5 μg; R&D Systems), or isotype control IgG1/Fc (5 μg; Jackson Immunoresearch) were injected (2 μl) into the cornea with a 33-gauge needle, as previously reported18 (link). The efficiency of corneal transfection by naked plasmid of pGFP (gift of X. Li, University of Kentucky) or placZ (gift of B.T. Spear, University of Kentucky) exceeded 70%, as gauged by flow cytometry and X-gal staining (Supplementary Fig. 11). We performed tail-vein injection of clodronate liposomes (200 μl) and intraperitoneal injection of anti-Gr-1 antibodies (200 μg; eBioscience) on each of the two days before and after corneal injection of pshRNA–sflt-1 to deplete peripheral monocytes/macrophages and neutrophils.
Publication 2006
5-bromo-4-chloro-3-indolyl beta-galactoside Antibodies, Neutralizing Clodronate Cornea Flow Cytometry FLT1 protein, human Goat IgG1 Immunoglobulin Isotypes Liposomes Macrophage Mus Needles Neutrophil Passive Immunization PBMC Peripheral Blood Mononuclear Cells Plasmids Short Hairpin RNA Tail Transfection Veins

Most recents protocols related to «Passive Immunization»

To deplete neutrophils, mice were treated with a 400 μg intraperitoneal injection of anti-mouse Ly6G antibody (108454, Biolegend) or IgG2b isotype control (400675, Biolegend) 24 h prior to injection of BSA-NPs. Subsequent 200 μg antibody injections were administered accompanied by BSA-NPs. Animals were euthanized after 16 h to collect peritoneal lavage fluid, ectopic lesions, and eutopic endometrium. Neutrophil depletion was confirmed by flow cytometry as described below.
Publication 2023
Animals Endometrium Flow Cytometry IgG2B Immunoglobulin Isotypes Mus Neutrophil Passive Immunization Peritoneal Fluid Peritoneal Lavage
30 μL 10 mg/ml FITC-BSA-NPs, ICG-BSA-NPs, FITC-BSA-GOx-NPs or ICG-BSA-GOx-NPs were i.p. or i.v. injected into endometriosis mice. After 16 h without further statement, the major organs of mice, including heart, liver, spleen, lung, kidney, eutopic endometrium and ectopic lesions, were collected and fluorescent images of these organs were acquired with IVIS. Moreover, 400 μg anti-Ly6G antibody or an IgG2b isotype control antibody was administered intraperitoneally 24 h prior to injection of FITC-BSA-NPs. Subsequent 200 μg antibody injections were intraperitoneal administered accompanied with FITC- BSA-NPs. Animals were euthanized after 16 h to collect the major organs and fluorescent images were acquired.
Publication 2023
Animals Antibodies, Anti-Idiotypic Endometriosis Endometrium fluorescein isothiocyanate bovine serum albumin Heart IgG2B Immunoglobulin Isotypes Immunoglobulins Kidney Liver Lung Mus Passive Immunization Spleen
Intravenous injection of anti-PD-1 antibodies was given: camrelizumab (200 mg), sintilimab (200 mg), or tislelizumab (200 mg) at 3-week intervals. In cases of severe TRAEs or disease progression, the drug was discontinued.
Publication 2023
camrelizumab Disease Progression Passive Immunization Pharmaceutical Preparations sintilimab tislelizumab
On days −3, −2, and −1, mice received a daily i.p. injection of either 500 μg anti-CD8a antibody (BioXCell, Cat. no. BE0004-1; RRID:AB_1107671) or 500 μg InVivoMAb rat IgG2a isotype control, anti-trinitrophenol (BioXCell, Cat. #BE0079). InVivoPure pH 6.5 dilution buffer (BioXCell, Cat. #IP0065) was used as a vehicle for both antibodies. On day 0, CD8 T cell depletion was validated via flow cytometry (Figure S5AB), and mice within each antibody group were randomized into four treatment groups based on IVIS total flux: IgG2a + Vehicle control (n = 9), Anti-CD8a antibody (n = 10), 150 mg/kg EZM8266 (n = 9), and Anti-CD8a + EZM8266 (n = 10). EZM8266 was administered daily via oral gavage, and 250 μg anti-CD8a antibody was administered via i.p. injection twice per week starting on day 1.
Publication Preprint 2023
Antibodies Antibodies, Anti-Idiotypic Buffers CD8-Positive T-Lymphocytes Flow Cytometry IgG2A Immunoglobulin Isotypes Immunoglobulins Mus Passive Immunization picric acid Technique, Dilution Tube Feeding
Tfh cells were depleted using a blockade of ICOS/ICOS-L signaling as described previously (38 (link)). Beginning at day 0, AQP4-immunized mice were given 150 μg in 200 μL PBS of anti–ICOS-L (clone HK5.3, Bio X Cell) or isotype control (clone 2A3, Bio X Cell) antibody via intraperitoneal injection every other day for 42 days (Figure 8A).
Publication 2023
Cells Clone Cells ICOS protein, human Immunoglobulin Isotypes Mus Passive Immunization T Follicular Helper Cells

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BALB/c mice are an inbred strain of laboratory mice commonly used in scientific research. They are a widely utilized model organism for various experiments and studies. The BALB/c strain is known for its susceptibility to certain diseases and its ability to produce high levels of antibodies, making it a valuable tool for immunological research.
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Anti-CD4 (clone GK1.5) is a monoclonal antibody that specifically binds to the CD4 cell surface receptor. It is a commonly used tool in immunological research and analysis.
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Complete Freund's adjuvant is a laboratory reagent used to enhance the immune response in laboratory animals during the production of antibodies. It contains inactivated and dried mycobacteria suspended in a mineral oil emulsion. The mycobacteria component serves to stimulate the animal's immune system, leading to a stronger and more sustained antibody response to the antigen of interest.
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The Anti-CD8 (clone 2.43) is a monoclonal antibody that binds to the CD8 surface marker. It is commonly used in flow cytometry and cell-based assays to identify and analyze CD8+ T cells.
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Rat IgG2b is an immunoglobulin subclass produced by rats. It is a laboratory reagent used for various research applications.
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The Clone 1A8 is a laboratory instrument designed for cell culture applications. It is a specialized device that facilitates the cloning and expansion of individual cells or cell lines. The core function of the Clone 1A8 is to enable the isolation, selection, and propagation of specific cell populations, supporting research and development activities in various fields.
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More about "Passive Immunization"

Passive immunization, also known as passive immunity, is a process in which antibodies or antigen-specific T-cells are transferred from an immune individual to a non-immune one.
This approach provides immediate, short-term protection against a particular pathogen or toxin.
Passive immunization can be used to prevent or treat infectious diseases, autoimmune disorders, and other conditions.
BALB/c mice, a commonly used murine model, are often employed in passive immunization studies.
Anti-CD4 (clone GK1.5, BP0075-1, BE0089) and Anti-CD8 (clone 2.43, BP0089) antibodies are frequently utilized to deplete specific T-cell subsets in these experiments.
Complete Freund's adjuvant may also be used to enhance the immune response.
PubCompare.ai's innovative AI-driven platform helps researchers easily identify the most promising passive immunization strategies by locating the best research protocols from literature, pre-prints, and patents using smart comparisons.
This allows researchers to optimize their workflow and experence the future of research today.