The molecular size of the rHSP27 and rC1 proteins with or without the PAb (using ratios of 1:1 and 1:5 for Hsp27:PAb; wt:wt) was determined by size exclusion chromatography in AKTA Primer Plus fast protein liquid chromatography system (GE Healthcare) with Superose TM 6 10/30 GL Column (GE Healthcare; Supplemental Fig. 1B). Samples were diluted in phosphate buffered saline (PBS) to 20 μg/ml and the PAb was mixed with rHSP27 for 30 mins. A final sample volume of 0.2 ml was loaded on the columns, the fractions were eluted with 0.2 ml/min PBS buffer and absorption was monitored at 280 nm. A standard curve was constructed using a mix of blue dextran (2,000 kD, Millipore Sigma, Oakville, ON), apoferritin (443 kD, Millipore Sigma,), Alcohol Dehydrogenase from yeast (150 kD; Millipore Sigma) and bovine serum albumin (BSA; 66kD), as well as human LDL and HDL (Biomedical Technologies).
Human ldl and hdl
Manufactured by Biomedical Technologies
Human LDL and HDL are laboratory equipment used for the analysis and measurement of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) levels in human samples. These lipoproteins are essential components of the body's cholesterol transport system.
Automatically generated - may contain errors
Lab products found in correlation
Akta primer plus fast protein liquid chromatography system, by GE Healthcare (2 mentions)
Superose tm 6 10 30 gl column, by GE Healthcare (2 mentions)
Blue dextran, by Merck Group (2 mentions)
Apoferritin, by Merck Group (2 mentions)
Alcohol dehydrogenase from yeast, by Merck Group (2 mentions)
Bovine serum albumin bsa, by Biomedical Technologies (2 mentions)
2 protocols using human ldl and hdl
1
Molecular Size Determination of Hsp27 and C1
2
Molecular Size Determination of Hsp27 and C1
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The molecular size of the rHSP27 and rC1 proteins with or without the PAb (using ratios of 1:1 and 1:5 for Hsp27:PAb; wt:wt) was determined by size exclusion chromatography in AKTA Primer Plus fast protein liquid chromatography system (GE Healthcare) with Superose TM 6 10/30 GL Column (GE Healthcare; Supplemental Fig. 1B). Samples were diluted in phosphate buffered saline (PBS) to 20 μg/ml and the PAb was mixed with rHSP27 for 30 mins. A final sample volume of 0.2 ml was loaded on the columns, the fractions were eluted with 0.2 ml/min PBS buffer and absorption was monitored at 280 nm. A standard curve was constructed using a mix of blue dextran (2,000 kD, Millipore Sigma, Oakville, ON), apoferritin (443 kD, Millipore Sigma,), Alcohol Dehydrogenase from yeast (150 kD; Millipore Sigma) and bovine serum albumin (BSA; 66kD), as well as human LDL and HDL (Biomedical Technologies).
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