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2 protocols using 1 palmitoyl 2 stearoyl sn glycero 3 phosphocholine

1

Phosphatidylcholine: Sourcing and Standards

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Phosphatidylcholine type S100 (soybean lecithin, phosphatidylcholine content ≥94%) and S-PC-3 (soybean lecithin, hydrogenated, phosphatidylcholine content ≥98%) were kindly provided by Lipoid GmbH, Ludwigshafen, Germany.
According to the manufacturer’s specification the fatty acids of the two acyl groups of phosphatidylcholine within the used lecithin types are listed in Table 1.
Phosphatidylcholine standards of 1,2-dilinoleoyl-sn-glycero-3-phosphocholine (18:2–18:2 PC, DLPC), 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (16:0–18:2 PC, PLPC), 1-palmitoyl-2-stearoyl-sn-glycero-3-phosphocholine (16:0−18:0 PC, PSPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (16:0–16:0 PC, DPPC) were purchased from Avanti Polar Lipids (Alabama, US). Phosphatidylcholine standards 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (16:0–18:1 PC, POPC), 1,2-dioleoyl-sn-glycero-3-phosphocholine (18:1–18:1 PC, DOPC) and 1,2-distearoyl-sn-glycero-3-phosphocholine (18:0–18:0 PC, DSPC) were supplied from Lipoid GmbH (Ludwigshafen, Germany).
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2

Lipid Compound Characterization and Preparation

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Lipid compounds including 1-palmitoyl-2-stearoyl-sn-glycero-3-phosphocholine (HSPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino (polyethyleneglycol)-2000] [DSPE-PEG(2000)], 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethyleneglycol)-2000]-folate [DSPE-PEG (2000)-folate], 1,2-dipalmitoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DPPG), and cholesterol were obtained from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Free folate; PFP (29°C BP); and fluorescent dyes including 4′,6-diamidino-2-phenylindole (DAPI), 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR), 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI), and 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO) were purchased from Sigma-Aldrich Chemical Co. (St Louis, MO, USA). Chloroform (CHCl3) was purchased from Fisher Scientific (Waltham, MA, USA).
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