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2 protocols using xad 16amberlite resin

1

Phytochemical Analysis of Maple Syrup

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All solvents
were either ACS or HPLC grade and were obtained from Pharmco-AAPER
through Wilkem Scientific (Pawcatuck, RI). Sephadex LH-20, XAD-16
Amberlite resin, 2,2-diphenyl-1-picrylhydrazyl (DPPH), the Folin–Ciocalteau
reagent, and all of the cell culture supplies were purchased from
Sigma-Aldrich (St. Louis, MO). Standards of compounds used to aid
in the phytochemical characterization of MSX were previously isolated
and identified (by NMR) from maple syrup and maple sap by our laboratory.4 (link)−7
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2

Anti-Oxidant and Anti-Inflammatory Assay Protocol

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Aminoguanidine hydrochloride (AG), bovine serum albumin (BSA), d-fructose, d-ribose, 2,3-dimethylquinoxaline (DQ), gallic acid (GA), resveratrol (RESV), butylated hydroxytoluene (BHT), 2,2-diphenyl-1-picrylhydrazyl (DPPH), methylglyoxal (MGO), 1,2-phenylenediamine (PD), hydrogen peroxide (H2O2), trifluoroacetic acid (TFA), lipopolysaccharide (LPS), 2’,7’-dichlorofluorescin diacetate reagent (DCFDA), XAD-16 Amberlite resin, and HPLC-grade methanol were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Beta amyloid 1-42 (Aβ) was purchased from AnaSpec Inc. (Fremont, CA, USA). Griess reagent was purchased from Promega Corp. (Madison, MI, USA).
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