Silicon

Manufactured by Kulzer

Sourced in United States

Silicon is a lab equipment product used in various scientific applications. It serves as a material for manufacturing components and devices used in laboratory settings. The core function of silicon is to provide a stable and reliable base for constructing and supporting various analytical and experimental setups.

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Lab products found in correlation

Isoflurane, by RWD Life Science (1 mentions) Laser doppler flowmetry, by Moor Instruments (1 mentions)

2 protocols using silicon

Transient Middle Cerebral Artery Occlusion in Mice

Cited 1 time

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tMCAO was carried out owing to previously published research [53 (link)]. Briefly, adult ICR mice (weighing 27 ± 3 g) were anesthetized with 1.5–2% isoflurane (RWD, Shenzhen, China) in a mixture of 30% oxygen and 70% nitrous oxide. The left common carotid artery, along with the internal carotid artery and the external carotid artery, were temporarily ligated. A cut was made between the two ligations on the external carotid artery. Following this, a 6-0 nylon suture (Covidien, Mansfield, MA, USA) coated with silicon (Heraeus Kulzer, Germany) was introduced through the incision and guided toward the ipsilateral middle cerebral artery. The success of middle cerebral artery occlusion was confirmed using laser Doppler flowmetry (Moor Instruments, Devon, UK) to observe a 10% reduction in the surface cerebral blood flow compared to its baseline level. The suture was withdrawn 1.5 h after the occlusion, and successful reperfusion was confirmed by observing the surface cerebral blood flow recovery back to 70% of its baseline level.

Zheng H., Haroon K., Liu M., Hu X., Xu Q., Tang Y., Wang Y., Yang G.Y, & Zhang Z. (2024). Monomeric CXCL12-Engineered Adipose-Derived Stem Cells Transplantation for the Treatment of Ischemic Stroke. International Journal of Molecular Sciences, 25(2), 792.

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Microsurgical Suture Implantation Protocol

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A 7-0 monofilament suture (Harvard Apparatus, Holliston, MA) was cut into 20 mm segments. The tip of each segment was embedded with silicon (Heraeus Kulzer, Inc., Armonk, NY). The diameter of the tip was measured using a micrometer (Applied Image Inc., Rochester, NY). We used a suture with a final tip diameter of 0.22–0.23 mm for mice with body weight in the range of 25–30 g. All surgical tools (Retractor; SuperCut Iris scissors, straight; Dumont forceps, fine tip, 45° bent tips; Vannas micro-scissors, straight; micro vessel clip, all from World Precision Instruments, Inc., Sarasota, FL, USA.) were sterilized by autoclaving (minimum 121 °C, 15 PSI, for 15 min). Sterile cotton tips (Fisher Scientific International, Inc. Pittsburgh, PA), Gauze sponges (Fisher Scientific International, Inc. Pittsburgh, PA), 70% ethanol, and sterile 0.9% saline were prepared. The surgery table was sanitized using 70% ethanol.

Xie L., Kang H, & Nedergaard M. (2016). A Novel Model of Transient Occlusion of the Middle Cerebral Artery in Awake Mice. Journal of nature and science, 2(2), e176.

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