Human decidual tissues were briefly fixed in 4% paraformaldehyde (PFA) and embedded in O.C.T. Compound (Catalog No. 4583, Sakura Finetek, Torrance, CA). The frozen sections at 10 μm were further fixed in 4% PFA and treated with 0.1% triton, and subjected to the incubation with specific antibodies against NCAM1/CD56 (Catalog No. ab75813, Abcam, Cambridge, MA), CK7 (Catalog No. ab181598, Abcam), CD39 (Catalog No. 14211-1-AP, Proteintech, Wuhan, China), or CD103 (Catalog No. 350227, BioLegend). Binding of the antibody was visualized using FITC-conjugated or TRITC-conjugated secondary antibody (Catalog No. ZF-0311 or ZF-0313, ZSGB-BIO, Beijing, China), and cell nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI; Catalog No. 28718-90-3, Sigma). Immunofluorescent staining was examined using Zeiss LSM780 confocal system (Carl Zeiss, Jena, Germany) and processed with ZEN 2012 software (Carl Zeiss). Immunohistochemical staining for CK in decidua was performed by using antibody against CK7 (Catalog No. ab181598, Abcam) and HRP-conjugated second antibody (Catalog No. PV-6001, ZSGB-BIO) followed by recovery of substrate diaminodbenzidine (DAB) (Catalog No. ZLI-9019, ZSGB-BIO). The imagines were recorded on a light microscope with charge-coupled device (CCD) (Olympus, Tokyo, Japan).
Catalog no 4583
Manufactured by Sakura Finetek
Sourced in Japan
Catalog No. 4583 is a laboratory equipment product. It is designed for use in scientific and research applications. The core function of this product is to [DESCRIPTION_NOT_AVAILABLE].
Automatically generated - may contain errors
2 protocols using catalog no 4583
1
Immunofluorescence and Immunohistochemical Analysis of Human Decidual Tissues
2
Retinal Ischemia-Reperfusion Injury in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
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At day 7 after RIR injury, each eyeball was enucleated and fixed in 4% paraformaldehyde at 4° C overnight. After fixation, the anterior segment of the eyeball was removed and the posterior segment was dehydrated in a graded sucrose series and embedded in O.C.T. compound (Catalog No. 4583; Sakura Finetek Co., Ltd., Tokyo, Japan). For HE staining, sections through the optic disc of the eye were cut into 6-µm-thick sections. Because retinal thickness in the mouse model is not uniform and is dependent upon location, all measurements were performed in the mid-peripheral retinal region 1 mm from the optic nerve head. All images were captured using a charge-coupled device camera (Olympus DP25; Olympus Corporation, Tokyo, Japan) connected to a light microscope (Olympus BX41; Olympus Corporation). The images were analyzed using Image-Pro Plus software (version 6.0.0.260; Media Cybernetics Inc., Bethesda, MD, USA). To assess ischemic damage in the retina, we measured the retinal thickness from the GCL to the outer plexiform layer. Retinal thickness was calculated as the average of six measurements in each eye. Eight mice from each group were histopathologically examined.
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