Log In Sign up
The largest database of trusted experimental protocols

Cell culture supplies

Manufactured by Merck Group
Visit Supplier
Sourced in United States

Cell culture supplies are essential equipment used in laboratories to support the growth and maintenance of living cells. These supplies include items such as culture dishes, flasks, plates, and other vessels designed to provide a controlled and sterile environment for cell cultures. They serve as the fundamental tools for conducting a wide range of cell-based research, experiments, and applications.

Automatically generated - may contain errors

Lab products found in correlation

Click chemistry reagents, by Vector Laboratories (2 mentions) Goat anti mouse igg h l alexa fluor 488, by Abcam (2 mentions) Click it cell reaction buffer kit, by Thermo Fisher Scientific (2 mentions) 3h 5 ht, by PerkinElmer (1 mentions) Cell culture dishes, by Sarstedt (1 mentions) 3h mpp, by American Radiolabeled Chemicals (1 mentions) Rabbit polyclonal anti calnexin, by Abcam (1 mentions) Goat anti rabbit igg h l alexa fluor 488, by Abcam (1 mentions)

5 protocols using cell culture supplies

1

Synthesis of 2-Chlorohexadecanal Analogs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell culture supplies were purchased from Sigma-Aldrich (St. Louis, MO, USA). Click chemistry reagents were purchased from Click Chemistry Tools, (Scottsdale, AZ, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Thermo Fisher Scientific (Waltham, MA, USA). The 2-ClFALD molecular species, 2-chlorohexadecanal (2-ClHDA) ω-alkyne analog (2-ClHDyA) was synthesized as previously described [15 ,[28] (link), [29] , [30] (link)].
Shakya S., Pyles K.D., Albert C.J., Patel R.P., McCommis K.S, & Ford D.A. (2022). Myeloperoxidase-derived hypochlorous acid targets human airway epithelial plasmalogens liberating protein modifying electrophilic 2-chlorofatty aldehydes. Redox Biology, 59, 102557.
+ Open protocol
+ Expand
2

Synthesis and Characterization of Fluorinated Morpholines

Check if the same lab product or an alternative is used in the 5 most similar protocols
2-(2-Fluorophenyl)-3-methylmorpholine (2-FPM), 2-(3-fluorophenyl)-3-methylmorpholine (3-FPM) and 2-(4-fluorophenyl)-3-methylmorpholine (4-FPM) were prepared as fumarate salts and analytically characterized previously (McLaughlin et al., 2016 (link)). [3H]5HT (28.3 µCi mmol−1) was purchased from PerkinElmer (Boston, MD, USA), and [3H]MPP+ (80–85 µCi mmol−1) was purchased from American Radiolabeled Chemicals (St. Louis, MO, USA). Cell culture dishes were from Sarstedt AG&Co., Nuembrecht, Germany. All other chemicals and reagents, including cell culture supplies, were purchased from Sigma Aldrich.
Mayer F.P., Burchardt N.V., Decker A.M., Partilla J.S., Li Y., McLaughlin G., Kavanagh P.V., Sandtner W., Blough B.E., Brandt S.D., Baumann M.H, & Sitte H.H. (2017). Fluorinated phenmetrazine “legal highs” act as substrates for high-affinity monoamine transporters of the SLC6 family. Neuropharmacology, 134(Pt A), 149-157.
+ Open protocol
+ Expand
3

Click Chemistry Reagents Synthesis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell culture supplies were purchased from Sigma-Aldrich (St. Louis, MO, USA). Click chemistry reagents were purchased from Click Chemistry Tools, (Scottsdale, AZ, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Thermo Fisher Scientific (Waltham, MA, USA). The alkyne analog of 2-ClHDA (2-ClHDyA) and 2-ClHA (2-ClHyA), were synthesized, according to the protocols described previously [17 (link),24 (link),25 (link)].
Shakya S., Herr R.A., Carlson H.L., Zoeller R.A., Albert C.J, & Ford D.A. (2022). Endothelial Cell Protein Targeting by Myeloperoxidase-Derived 2-Chlorofatty Aldehyde. Antioxidants, 11(5), 940.
+ Open protocol
+ Expand
4

Immunofluorescence Staining of Cell Adhesion Molecules

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell culture supplies were purchased from Sigma-Aldrich (Vienna, Austria), Lonza (Basel, Switzerland), or Cell Applications Inc. (San Diego, CA, United States). Click-It Cell Reaction Buffer Kit was purchased from Thermo Fisher Scientific (Waltham, MA, United States; catalog no. C10269). Rabbit polyclonal anti-calnexin (catalog no. ab22595), rabbit monoclonal anti- voltage-dependent anion-selective channel 1/porin antibody (anti-VDAC1; catalog no. ab154856), mouse polyclonal anti-golgi matrix protein 130 (anti-GM130; catalog no. ab169276), mouse monoclonal anti-Von Willebrand Factor (anti-VWF; catalog no. ab20435), goat anti-rabbit IgG H&L (Alexa Fluor® 488) (catalog no: 150077), and goat anti-mouse IgG H&L (Alexa Fluor® 488) (catalog no. ab150113) antibodies were purchased from Abcam (Cambridge, United Kingdom). Antibodies to adhesion molecules (mouse monoclonal anti-P-selectin, sc-18834; mouse monoclonal anti-E-selectin, sc-5262; mouse monoclonal anti-intercellular adhesion molecule-1, ICAM-1, sc-53336; mouse monoclonal anti-vascular cell adhesion molecule-1, VCAM-1, sc-13160) were purchased from Santa Cruz Biotechnology (Dallas, TX, United States). All other chemicals were purchased from Sigma-Aldrich or Thermo Fisher Scientific.
McHowat J., Shakya S, & Ford D.A. (2020). 2-Chlorofatty Aldehyde Elicits Endothelial Cell Activation. Frontiers in Physiology, 11, 460.
+ Open protocol
+ Expand
5

Fluorescent Labeling of Organelles

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell culture supplies were purchased from Sigma-Aldrich (St. Louis, MO, USA). A Click-It Cell Reaction Buffer Kit was purchased from Thermo Fisher Scientific (Waltham, MA, USA; catalog no. C10269). Rabbit polyclonal anti-calnexin (catalog no. ab22595), anti-cytochrome c oxidase subunit IV (anti-COX IV; catalog no. ab202554), mouse polyclonal anti-Golgi matrix protein 130 (anti-GM130; catalog no. ab169276), goat anti-rabbit IgG H&L (Alexa Fluor® 488) (catalog no: 150077) and goat anti-mouse IgG H&L (Alexa Fluor® 488) (catalog no. ab150113) antibodies were purchased from Abcam (Cambridge, UK). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Thermo Fisher Scientific (Waltham, MA, USA). The alkyne analog of 2-ClHDA (2-ClHDyA) was synthesized and quantified as described previously [15 (link),18 (link),19 (link),20 (link)].
Shakya S., McGuffee R.M, & Ford D.A. (2023). Characterization of N-Acetyl Cysteine Adducts with Exogenous and Neutrophil-Derived 2-Chlorofatty Aldehyde. Antioxidants, 12(2), 504.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!