Alamethicin, β-estradiol, dipyridamole (DIPY), D-saccharic-1, 4-lactone, magnesium chloride (MgCl2), MK-571, Ko143, leukotriene C4 (LTC4) and uridine diphosphate glucuronic acid (UDPGA) were all obtained from Sigma-Aldrich (St Louis, MO). Apigenin, chrysin, cycloicaritin, genistein, and wushanicaritin with purity over 98% was purchased from Aladdin Reagents (Shanghai, China). Apigenin-7-O-glucuronide, chrysin-7-O-glucuronide, genistein-7-O-glucuronide, and β-estradiol-3-O-glucuronide was purchased from Toronto Research Chemicals (North York, ON, Canada). Wushanicaritin-3-O-glucuronide was prepared in our laboratory as described previously [23 (link)]. 293T cells, HeLa cells, pLVX-mCMV-ZsGreen-PGK-Puro vector [9371 base pairs (bp)] and pLVX-shRNA2-Neo vector (9070 bp) were all provided from BioWit Technologies (Shenzhen, China). The pGEM-T plasmid which carried the UGT1A1 cDNA clone was purchased from Sino Biological Inc. (Beijing, China), while recombinant human UGT1A1 was purchased from Corning Biosciences (New York, USA). The anti-BCRP, anti-GAPDH, anti-MRP1, anti-MRP2, anti-MRP3, anti-MRP4 and anti-UGT1A1 antibodies were all obtained from OriGene Technologies (Rockville, MD). All other chemicals and reagents were the highest grade commercially available.
Anti bcrp
Manufactured by OriGene
Sourced in United States
Anti-BCRP is a laboratory reagent used to detect and quantify the expression of the BCRP (Breast Cancer Resistance Protein) protein. It is a useful tool for researchers studying multidrug resistance in cancer cells.
Automatically generated - may contain errors
Lab products found in correlation
Ko143, by Merck Group (3 mentions)
Anti mrp1, by OriGene (3 mentions)
Anti mrp2, by OriGene (3 mentions)
Anti mrp3, by OriGene (3 mentions)
Anti mrp4, by OriGene (3 mentions)
Alamethicin, by Merck Group (3 mentions)
β estradiol 3 o glucuronide, by LGC (2 mentions)
Mk571, by Merck Group (2 mentions)
Uridine diphosphate glucuronic acid, by Merck Group (2 mentions)
Propofol, by Merck Group (2 mentions)
D saccharic 1 4 lactone, by Merck Group (2 mentions)
D saccharic 1, by Merck Group (1 mentions)
Magnesium chloride mgcl2, by Merck Group (1 mentions)
Anti gapdh, by OriGene (1 mentions)
4 lactone, by Merck Group (1 mentions)
Dipyridamole dipy, by Merck Group (1 mentions)
Leukotriene c4 ltc4, by Merck Group (1 mentions)
Uridine diphosphate glucuronic acid udpga, by Merck Group (1 mentions)
β estradiol, by Merck Group (1 mentions)
β estradiol, by LGC (1 mentions)
3 protocols using anti bcrp
1
Glucuronidation Profiling of Flavonoids
2
Demethoxycurcumin Glucuronidation by UGT1A1
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Demethoxycurcumin (DMC) was provided by Guangzhou Fans Biotechnology Co., Ltd. (Guangzhou, China). Alamethicin, D-saccharic-1,4-lactone, magnesium chloride (MgCl2), MK571, Ko143, propofol, and uridine diphosphate glucuronic acid (UDPGA) were purchased from Sigma-Aldrich (St. Louis, MO). Human UGT1A1 and individual human liver microsomes (iHLM, n = 12) were obtained from Corning Biosciences (New York, USA). UGT1A1-overexpressing HeLa cells (HeLa1A1 cells) were established as described in a previous study [22 (link)] and provided by Prof. Baojian Wu, who works in Jinan University in Guangzhou of China. β-Estradiol and β-Estradiol-3-O-glucuronide were purchased from Toronto Research Chemicals (North York, ON, Canada). The anti-UGT1A1 antibody was purchased from BD Biosciences (Woburn, MA). The anti-BCRP (catalog number TA322704), anti-MRP1 (catalog number TA309559), anti-MRP2 (catalog number TA313641), anti-MRP3 (catalog number TA314800), and anti-MRP4 (catalog number TA327332) antibodies were purchased from OriGene Technologies (Rockville, MD). All other chemicals and reagents (analytical grade or better) were commercially available.
3
Establishment and Characterization of UGT1A9-Overexpressing HeLa Cells
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Human UGT1A9-overexpressing HeLa cells (HeLa1A9 cells) were established as described previously (Jiang et al., 2011 (link)), and were provided by Prof. Baojian Wu who works in Jinan University in Guangzhou of China. Alamethicin, D -saccharic-1,4-lactone, magnesium chloride (MgCl2), MK571, Ko143, propofol, and uridine diphosphate glucuronic acid (UDPGA) were purchased from Sigma-Aldrich (St Louis, MO, United States), whereas propofol-O-glucuronide from Toronto Research Chemicals (North York, ON, Canada). Fraxetin was provided by Guangzhou Fans Biotechnology Co., Ltd., (Guangzhou, China), and human UGT1A9 was purchased from Corning Biosciences (New York, NY, United States). UGT1A9 antibody was purchased from BD Biosciences (Woburn, MA, United States). The anti-BCRP, anti-MRP1, anti-MRP2, anti-MRP3, and anti-MRP4 antibodies were purchased from OriGene Technologies (Rockville, MD, United States). All other chemicals and reagents (analytical grade or better) were commercially available.
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