Mouse hypothalamic protein lysates (20 µg protein) were separated by 10% (w/v) sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (Bio-Rad). Following blocking in 5% skim milk, the membranes were incubated overnight at 4 °C with primary antibodies against IFT88 (1:1000). Blots were developed using a horseradish peroxidase-linked secondary antibody (1:1000) and the chemiluminescent detection system (PerkinElmer). Bands were quantified with a densitometer (VersaDoc Multi Imaging Analyzer System; Bio-Rad) and normalized to the density of β-actin.
Versadoc multi imaging analyzer system
Manufactured by Bio-Rad
The VersaDoc Multi Imaging Analyzer System is a versatile lab equipment designed for imaging and analyzing a variety of samples. It provides high-quality image capture and advanced data analysis capabilities for various applications in the scientific and research community.
Automatically generated - may contain errors
Lab products found in correlation
Polyvinylidene difluoride membrane, by Bio-Rad (1 mentions)
Chemiluminescent detection system, by PerkinElmer (1 mentions)
Pvdf membrane, by GE Healthcare (1 mentions)
Sc 47778, by Santa Cruz Biotechnology (1 mentions)
Ag 20a 0034, by Adipogen (1 mentions)
Rt pcr kit, by Takara Bio (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
3 protocols using versadoc multi imaging analyzer system
1
Western Blot Analysis of IFT88 in Mouse Hypothalamus
2
Quantifying Hypothalamic NAMPT Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary hypothalamic astrocyte lysates (50 μg protein) were separated by 10% SDS-PAGE and transferred to a PVDF membrane (GE Healthcare). Following incubation in blocking buffer, the membranes were incubated overnight at 4 °C with an antibody against NAMPT (1:1500, mouse; Adipogen, AG-20A-0034). The blots were developed using a densitometer (VersaDoc Multi Imaging Analyzer System, Bio-Rad) and normalized using β-actin immunoblotting (1:1000, mouse; Santa Cruz, sc-47778).
3
Quantification of ApoJ mRNA Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was isolated by the Trizol Reagent (Invitrogen, CA). Single strand cDNA from total RNA was synthesized by reverse transcription (RT-PCR) kit (Clontech, Mountain View, CA) according to the kit instruction. For ApoJ mRNA, PCR reaction was performed in a 20 μl volume containing 10 ng of cDNA for 35 cycles. The forward primer 5ʹ-ACA ATG GCA TGG TCC TGG GAG AG-3ʹ and the reverse primer 5ʹ-GTA TGC TTC AGG CAG GGC TTG C-3ʹ were used. The PCR products were separated on 0.9% agarose gels and analyzed with gel-imaging system (VersaDoc Multi Imaging Analyzer System, Bio-Rad Laboratories, Hercules, CA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!