Alexa fluor 488 conjugated rabbit anti mouse igg secondary antibody

Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), bovine calf serum (BS), penicillin/streptomycin and trypsin 0.25% were purchased from Hyclone (GE Healthcare Life Sciences, Pittsburgh, PA, United States). 10X solution of phosphate buffered saline (PBS), SN-38, and Alexa Fluor 488 conjugated rabbit anti-mouse IgG secondary antibody were purchased from Thermo Fisher Scientific Inc. (Rockford, IL, United States). The monoclonal antibodies for ABCG2 (BXP-34), paclitaxel, vincristine, vinblastine, cisplatin, MX, verapamil, 3-(4, 5-dimethylthiazol-yl)-2, 5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), propidium iodide (PI), and Triton X-100, were obtained from Sigma Chemical Co. (St. Louis, MO, United States). Ko-143 and MK-571 were products from Enzo Life Sciences (Farmingdale, NY, United States). Monoclonal antibodies sc-47778 (against β-actin) and secondary HRP-labeled rabbit anti-mouse IgG were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, United States). [³H]- MX) (2.5 Ci/mmol) was purchased from Moravek Biochemicals (Brea, CA, United States). Liquid scintillation cocktail was a product from MP Biomedicals, Inc. (Santa Ana, CA, United States). All other chemicals were purchased from Sigma Chemical Co. (St. Louis, MO, United States).

The antimicrobial peptides were synthesized at and provided by Hunan University of Science and Engineering, Yongzhou, Hunan, China. Bovine calf serum (BS), fetal bovine serum (FBS), Dulbecco’s modified Eagle’s medium (DMEM), trypsin 0.25% and penicillin/streptomycin were purchased from Hyclone (GE Healthcare Life Sciences, Pittsburgh, PA). [³H]-paclitaxel (15 Ci/mmol) was purchased from Moravek Biochemicals (Brea, CA). Phosphate buffered saline (PBS), GAPDH Mouse Anti-Human Clone: GA1R, 4′,6-diamidino-2-phenylindole (DAPI), and Alexa Fluor 488 conjugated rabbit anti-mouse IgG secondary antibody were purchased from Thermo Fisher Scientific Inc. (Rockford, IL). Anti-mouse IgG HRP-linked secondary-antibody was purchased from Cell Signaling Technology (Danvers, MA). were obtained from Sigma Chemical Co. (St. Louis, MO). Liquid scintillation cocktail was a product of MP Biomedicals, Inc (St. Ana, CA). The monoclonal anti-P-glycoprotein (MDR) antibody Clone F4 produced in mice, paclitaxel, verapamil, cisplatin, 3-(4, 5-dimethylthiazol-yl)-2, 5-diphenyltetrazolium bromide (MTT), Triton X-100, dimethyl sulfoxide (DMSO), and all other chemicals were purchased from Sigma Chemical Co (St. Louis, MO).