Treating tissues and UC cells with 500 μL of cell lysis buffer (Cell Signaling, Cambridge, UK), and the protein concentration was measured by ELx800 spectrophotometer (BIO-TEK™; Wolf Laboratories, York, UK) using DC Protein Assay kit (Bio-Rad, Hemel Hemstead, England, UK). 50 ng of protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred into PVDF membrane. 2 hours after immunostaining with primary antibody, membrane was incubated with HRP-conjugated secondary antibody for 4 h Finally, protein bands were visualized using the enhanced chemiluminescence (ECL) system (Amersham, Aylesbury, UK), and photographed using an UVITech imager (UVITech, Inc., Cambridge, UK). LEF1 (# C12A5) rabbit mAb, β-catenin (# D10A8) XP® rabbit mAb, phospho-β-catenin (Ser33/37), MMP-2 (# D8N9Y) rabbit mAb, MMP-9 (# D6O3H) XP® rabbit mAb and anti-rabbit HRP-linked antibody (# 7074) were purchased from Cell Signaling Technology. PPAR gamma antibody (# ab59256), calpain 2 antibody (# ab39165) and β-actin monoclonal antibody (# ab6276) were purchased from Abcam (Cambridge, UK).
Mmp 9 d6o3h xp rabbit mab
Manufactured by Cell Signaling Technology
MMP-9 (# D6O3H) XP® rabbit mAb is a laboratory reagent used to detect and quantify the Matrix Metalloproteinase-9 (MMP-9) protein. MMP-9 is an enzyme involved in the breakdown of extracellular matrix proteins. This antibody can be used in various immunoassay techniques to analyze MMP-9 expression and levels.
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Lab products found in correlation
Ecl system, by Cytiva (2 mentions)
β actin monoclonal antibody ab6276, by Abcam (2 mentions)
Dc protein assay kit, by Bio-Rad (1 mentions)
Cell lysis buffer, by Cell Signaling Technology (1 mentions)
β catenin d10a8 xp rabbit mab, by Cell Signaling Technology (1 mentions)
Anti rabbit hrp linked antibody 7074, by Cell Signaling Technology (1 mentions)
Mmp 2 d8n9y rabbit mab, by Cell Signaling Technology (1 mentions)
2 protocols using mmp 9 d6o3h xp rabbit mab
1
Western Blot Protein Analysis
2
Protein Expression Profiling in Cells
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Total protein was isolated and an equal quantity of protein lysates (50 g/well) were performed by SDS‐polyacrylamide gel electrophoresis, then protein was electrotransferred onto polyvinylidene difluoride membranes. The membrane was incubated with a blocking solution (TBS, 0.1% Tween 20 and 5% nonfat dried milk powder) to block nonspecific protein for 2 hour at room temperature. Primary antibody of TGFBI (D31B8) XP Rabbit mAb, MMP‐2 (D8N9Y) Rabbit mAb and MMP‐9 (D6O3H) XP Rabbit mAb were purchased from Cell Signaling Technology. Calpain 2 antibody (ab39165) and β‐actin monoclonal antibody (ab6276) were purchased from Abcam. The blots were incubated with antibodies overnight at room temperature, and the immune complex was detected using a peroxidase‐conjugated secondary antibody and ECL system (Amersham) according to the manufacturer's instructions.
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