N n methylene bisacrylamide

N,N' methylene bisacrylamide was from Promega (Madison, WI, USA). Molecular mass markers for SDS polyacrylamide gel electrophoresis (SDS PAGE) and nitrocellulose sheets (0.22 μm pores, 100 % nitrocellulose) were from GE Healthcare (Buckinghamshire, UK). Paraformaldehyde was from Electron Microscopy Sciences (Hatfield, PA, USA), and glutaraldehyde was from TAAB (Reading, UK). L-[ 3 H]glutamic acid (52 Ci/mmol) and [ 3 H]GABA (89 Ci/mmol) were from Amersham (Buckinghamshire, UK). Sodium dodecyl sulfate (SDS) of high purity (>99 % C12 alkyl sulfate) and nitrocellulose filters (HAWP; 0.45 μm pores) were from Millipore (Carrigtwohill, C. Cork, Ireland). Electrophoresis equipment was from Hoefer Scientific Instruments (San Francisco, CA, USA). All other reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA).

Sodium dodecyl sulfate (SDS) of high purity (>99 % C12 alkyl sulfate) was from Pierce (Rockford, IL, USA), and electrophoresis equipment were from Hoefer Scientific Instruments (San Francisco, CA, USA). N,N'-methylene-bisacrylamide, acrylamide, ammonium persulfate, TEMED and alkaline phosphatase substrates (nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate) were from Promega (Madison, WI, USA). Molecular mass markers for SDS-polyacrylamide gel electrophoresis and nitrocellulose sheets (0.22 μm pores, 100 % nitrocellulose) were from Amersham (Buckinghamshire, UK). Prolong Gold AntiFade Mountant with was from Life Technologies (Carlsbad, CA, USA). Paraformaldehyde and glutaraldehyde EM grade were from TAAB (Reading, UK). All other chemicals were obtained from Sigma (St. Louis, MO, USA).