The reagents and analysis systems used were as follows: heroin (purity 92.09%, provided by the Guizhou Public Security Bureau), pentobarbital sodium (Sigma, USA), naloxone hydrochloride (Chongqing YaoPharma, China), mouse monoclonal anti-SIRT1 (Abcam, USA), rabbit polyclonal anti-Cdk5, rabbit polyclonal anti-NF-κB (Bioss, China), rabbit monoclonal anti-FOXO1 (Abcam, USA), rabbit polyclonal anti-Cbp (Bioss, China), rabbit monoclonal anti-PSD95 (Abcam, USA), rabbit monoclonal anti-Syn (Abcam, USA), mouse monoclonal anti-beta-actin (Abcam, USA), SuperScript III RT reverse transcription kit (Invitrogen, USA) Sybr qPCR mix (Invitrogen, USA), Plasmid Mini kit (TransGen Biotech, Beijing, China), gel extraction kit (Omega), Trans2K Plus II DNA Marker (TransGen, Beijing, China), DNA primers (Invitrogen, USA), DNA sequencing (Invitrogen, USA), restriction endonuclease EcoRI (NEB, USA), T4 DNA Ligase (NEB, USA), Plasmid Maxi Kit (Qiagen, Germany), CPT High-efficiency Transfection Kit (Virotherapy Technologies, Wuhan, China).
Restriction endonuclease ecori
Manufactured by New England Biolabs
Sourced in United States
EcoRI is a type II restriction endonuclease enzyme that recognizes and cleaves the DNA sequence 5'-GAATTC-3'. It is used in various molecular biology applications, such as DNA cloning, gene expression analysis, and genome manipulation.
Automatically generated - may contain errors
Lab products found in correlation
Pentobarbital sodium, by Merck Group (1 mentions)
Dna primers, by Thermo Fisher Scientific (1 mentions)
Mouse monoclonal anti beta actin, by Abcam (1 mentions)
Plasmid mini kit, by Transgene (1 mentions)
Trans2k plus 2 dna marker, by Transgene (1 mentions)
Sybr qpcr mix, by Thermo Fisher Scientific (1 mentions)
Dna sequencing, by Thermo Fisher Scientific (1 mentions)
Plasmid maxi kit, by Qiagen (1 mentions)
T4 dna ligase, by New England Biolabs (1 mentions)
Agarose, by Merck Group (1 mentions)
Cisplatin, by Merck Group (1 mentions)
Rnase a, by Qiagen (1 mentions)
α 32p atp, by MP Biomedicals (1 mentions)
2 protocols using restriction endonuclease ecori
1
Molecular Mechanisms of Heroin-Induced Neurotoxicity
2
Preparation and Characterization of DNA Complexes
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Commercial reagent grade chemicals, solvents and reagents for the preparation of solutions for biological work were used as received from commercial suppliers without further purification. Calf thymus (CT) DNA (42% G + C, mean molecular mass ca. 2 × 10 7 ) was prepared and characterized as previously described [14, 15] . Plasmid pPUC19 [2686 base pairs (bp)] was isolated according to standard procedures. Restriction endonuclease EcoRI was purchased from New England Biolabs. Agarose was obtained from Merck KgaA (Darmstadt, Germany). MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] was from Calbiochem (Darmstadt, Germany). [α-32 P]ATP was obtained from MP Biomedicals, LLC (Irvine, CA). RNase A was from Qiagen (USA). DNazol (genomic DNA isolation reagent) was obtained from MRC (Cincinnati, OH). Cisplatin was from Sigma-Aldrich (Prague, Czech Republic). Complexes 1 and 2 were synthesized and characterized as described in the previously published article [11] .
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