Anti mouse igg1κ pe

Manufactured by BD

Sourced in United States

Anti-Mouse IgG1κ-PE is a laboratory reagent used for the detection and quantification of mouse IgG1κ isotype antibodies in various immunological assays. It is a fluorescently labeled secondary antibody that specifically binds to the Fc region of mouse IgG1κ antibodies, allowing for their identification and measurement. The 'PE' in the name refers to the phycoerythrin fluorescent dye conjugated to the antibody, which emits a red-orange fluorescent signal upon excitation.

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2 protocols using anti mouse igg1κ pe

Multiparametric Immunophenotyping of Immune Cells

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The anti-mouse antibodies, anti-TER119-FITC, anti-CD4 FITC, anti-CD24-PE, anti-CD44-PE-Cy7, and anti-CD25-APCCy7, were purchased from eBioscience (San Diego, CA, USA). Anti-CD8-FITC, anti-CD8-AF647, and anti-CD4-Pacific Blue were purchased from BD Biosciences (San Jose, CA, USA). The anti-human antibodies, anti-CD1a-PerCP-Cy5.5, anti-CD1a-PECy5, anti-CD3-APCCy7, anti-CD4-Pacific Blue, anti-CD7-FITC, anti-CD8-BV785, Anti-CD8-FITC, anti-CD34-PE, anti-CD34-PECy7, and anti-CD38-AF700 were purchased from Biolegend (San Diego, CA). Anti-Aiolos-PE, anti-Eos-PE, and anti-Helios-AF647 were purchased from e-Bioscience. Anti-Mouse IgG1κ-PE and anti-Ikaros-PE were purchased from BD Biosciences. The anti-Armenian Hamster IgG-AF647 control was purchased from Biolegend.

Mitchell J.L., Seng A, & Yankee T.M. (2017). Expression and splicing of Ikaros family members in murine and human thymocytes. Molecular immunology, 87, 1-11.

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Activated T Cell PD-1 Binding Assay

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To prepare activated T cells, freshly isolated human PBMCs (2×10⁶ cells/ml) were stimulated with 10 ng/ml phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, St Louis, MO, USA) and 1 µg/ml ionomycin (Ion.; Sigma-Aldrich) in a volume of 200 µl RPMI-1640 containing 10% FBS at 37 °C in a humidified 5% CO₂ incubator for 24 h. Cells were collected and washed twice with FACS buffer, and added anti-human PD-1-PE (BD Pharmingen) and hPD-L1, L3B3-hPD-L1 and L3C7-hPD-L1 respectively at serial concentrations (0.02 µM, 0.04 µM , 0.2 µM , 0.411 µM , 2 µM , 4 µM , 8 µM and 20 µM) in the same time, and incubated in room temperature for 1 h. Samples added with anti-human PD-1-PE (BD Pharmingen) and anti-mouse IgG1κ-PE (BD Pharmingen) were considered as positive control and negative control respectively. Fluorescence was evaluated and the data was analyzed as above.

Liang Z., Tian Y., Cai W., Weng Z., Li Y., Zhang H., Bao Y, & Li Y. (2017). High-affinity human PD-L1 variants attenuate the suppression of T cell activation. Oncotarget, 8(51), 88360-88375.

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