Opal automation mif detection kit

Manufactured by Akoya Biosciences

Sourced in Japan

The Opal automation mIF detection kit is a laboratory equipment product designed for multiplexed immunofluorescence (mIF) detection. It automates the process of staining and imaging multiple markers in a single tissue sample, allowing for the simultaneous visualization of multiple cellular components.

Automatically generated - may contain errors

Lab products found in correlation

Anti pd l1, by Cell Signaling Technology (3 mentions) Anti pd 1, by Cell Signaling Technology (3 mentions) Anti cd8, by Abcam (3 mentions) Anti panck, by Abcam (3 mentions) Anti cd68, by Abcam (3 mentions) Anti cd163, by Abcam (3 mentions) Vectra polaris automated quantitative pathology imaging system, by Akoya Biosciences (2 mentions)

3 protocols using opal automation mif detection kit

Multiparametric Immunofluorescence Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formalin-fixed paraffin-embedded tumor tissue sections were prepared. Samples were stained using an Opal automation mIF detection kit (Akoya, Tokyo, Japan). The following antibodies were used: anti-CD163 (Abcam, Cat#ab182422), anti-CD8 (Abcam, Cat# ab178089), anti-CD68 (Abcam, Cat#ab213363), anti-PD-1 (Cell Signaling Technology, Cat#86183S), anti-PD-L1 (Cell Signaling Technology, Cat#13684S), and anti-panCK (Abcam, Cat#ab7753), CD20 (Daco, Cat#L26 IR604), CD3 (Daco, Cat#A0452 IR503), CD56 (Abcam, Cat#ab75813), CD4 (Abcam, Cat# ab133616), FoxP3 (Abcam, Cat# ab20034), and panCK. The Vectra Polaris Automated Quantitative Pathological Imaging System (Akoya) was used to overlay false colors on images from different channels. Tumor and stroma areas were divided according to cytokeratin (CK)-labeled tumor cells and nuclei stained with 4′-6′-diamidino-2-phenylindole (DAPI). Results are reported as percentages (immune subset cells/total cells of DAPI).

Xiang X., Guo F., Li G., Ma L., Zhu X., Abdulla Z., Li J., Zhang J, & Huang M. (2023). Efficacy of intra-arterial chemotherapy with sequential anti-PD-1 antibody in unresectable gastric cancer: A retrospective real-world study. Frontiers in Oncology, 12, 1015962.

+ Open protocol

+ Expand

Multiplex Immunofluorescence Profiling of Tumor Microenvironment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formalin-fixed paraffin-embedded tumor tissue paired slides were prepared. Samples were stained using an Opal automation mIF detection kit (Akoya, Tokyo, Japan). A total of 6 markers were labeled in one seven-color multiplex panel. The following antibodies were used for one panel: anti-CD163 (Abcam Cat#ab182422), anti-CD8 (Abcam Cat# ab178089), anti-CD68 (Abcam Cat#ab213363), anti-PD-1 (Cell Signaling Technology Cat#86183S), anti-PD-L1 (Cell Signaling Technology Cat#13684S), and anti-panCK (Abcam Cat#ab7753). The labeled slides were scanned using a Vectra Polaris Automated Quantitative Pathology Imaging System (Akoya), and images from different channels were false-colored and superimposed. Tumor and stromal areas were divided based on CK-labeled tumor cells, and the cell nuclei were counterstained with 4′–6′-diamidino-2-phenylindole (DAPI). The results are reported as percentages (immune subset cells/total cells of DAPI) and density (cells/mm2) of each individual cell subpopulation in the tumor or stromal area.

Gong C., Zhang W., Sun Y., Shou J., Jiang Z., Liu T., Wang S., Liu J., Sun Y, & Zhou A. (2023). Exploration of the immunogenetic landscape of hyperprogressive disease after combined immunotherapy in cancer patients. iScience, 26(6), 106720.

+ Open protocol

+ Expand

Multiplex Immunofluorescence Analysis of Tumor Immune Landscape

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formalin-fixed paraffin-embedded tumor tissue slides at baseline were prepared. Samples were stained using an Opal automation mIF Detection Kit (Akoya). A total of 11 markers were labeled in two seven-color multiplex panels. The following antibodies were used: anti-CD163 (Abcam, Catalog no. ab182422), anti-CD8 (Abcam, Catalog no. ab178089), anti-CD68 (Abcam, Catalog no. ab213363), anti–PD-1 (Cell Signaling Technology, Catalog no. 86183S), anti-PD-L1 (Cell Signaling Technology, Catalog no. 13684S), and anti-panCK (Abcam, Catalog no. ab7753) for panel 1, as well as CD20 (Daco, Catalog no. L26 IR604), CD3 (Daco, Catalog no. A0452 IR503), CD56 (Abcam, Catalog no. ab75813), CD4 (Abcam, Catalog no. ab133616), FoxP3 (Abcam, Catalog no. ab20034), and panCK for panel 2. The labeled slides were scanned using a Vectra Polaris Automated Quantitative Pathology Imaging System (Akoya), and images from different channels were false-colored and superimposed. Tumor and stroma areas were divided according to cytokeratin (CK)-labeled tumor cells and the cell nuclei counterstained with 4′-6′-diamidino-2-phenylindole (DAPI). Results are reported as percentages (immune subset cells/total cells of DAPI) and density (cells/mm²) from each individual cell subpopulation in the tumor or stromal area.

Zhang W., Gong C., Peng X., Bi X., Sun Y., Zhou J., Wu F., Zeng H., Wang Y., Zhou H., Zhao H., Cai J, & Zhou A. (2022). Serum Concentration of CD137 and Tumor Infiltration by M1 Macrophages Predict the Response to Sintilimab plus Bevacizumab Biosimilar in Advanced Hepatocellular Carcinoma Patients. Clinical Cancer Research, 28(16), 3499-3508.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!