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Cxcr5 pe cy7

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CXCR5-PE-Cy7 is a fluorescently conjugated antibody that detects the CXCR5 receptor. CXCR5 is a chemokine receptor that plays a role in B-cell migration and organization of lymphoid tissues. The PE-Cy7 fluorescent label allows for detection and analysis of CXCR5-expressing cells using flow cytometry.

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Lab products found in correlation

Live dead fixable near ir dead cell stain kit, by Thermo Fisher Scientific (3 mentions) Cd25 bv421, by BD (3 mentions) Cd8 pe, by BD (3 mentions) Cd14 apc cy7, by BD (3 mentions) Cd19 bv510, by BioLegend (3 mentions) Cd19 apc cy7, by BD (3 mentions) Cd3 apc cy7, by BioLegend (3 mentions) Cd3 buv395, by BD (3 mentions) Cd21 apc, by BioLegend (3 mentions) Cd4 buv496, by BD (3 mentions) Cd38 pe cy7, by BioLegend (3 mentions) Cd27 bv650, by BioLegend (3 mentions) Pd 1 bv711, by BioLegend (3 mentions) Cd10 pe dazzle594, by BioLegend (3 mentions) Igd buv 737, by BD (3 mentions) Igg percp, by BD (3 mentions) Iga pe, by Miltenyi Biotec (3 mentions) Lsr 2 flow cytometer, by BD (1 mentions) Cd4 pe, by BD (1 mentions) Facsdiva software, by BD (1 mentions)

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CXCR5-PE (5 citations)

4 protocols using cxcr5 pe cy7

1

Comprehensive B and T Cell Profiling

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Donor PBMCs was stained with live-dead marker (1:1000; Invitrogen, LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit) to gate out the dead cells for 20 min in 4 °C. After washing with FACS buffer, the cells were stained with respective B, T cell marker panel antibodies diluted in FACS buffer (1xPBS, 2% FBS, 0.1% sodium azide) at 4 °C for 30 min. B cell panel: CD19-BV510 (1:40; Biolegend, Cat. No. 363020), CD14-APC-Cy7 (1:50; BD, Cat. No. 557831), CD3-APC-Cy7 (1:125; Biolegend, Cat. No. 317342), CD10-PE-Dazzle594 (1:50; Biolegend, Cat. No. 312228), CD21-APC (1:50; Biolegend, Cat. No. 354906), CD27-BV650 (1:40; Biolegend, Cat. No. 302828), CD38-PE-Cy7 (1:40; Biolegend, Cat. No. 356608), PD-1-BV711 (1:20; Biolegend, Cat. No. 329928), IgD-BUV 737 (1:40; BD, Cat. No. 612798), IgG-PerCP (1:40; BD), IgA-PE (1:40; Miltenyi, Cat. No. 130-114-002). T cell panel: CD14-APC-Cy7 (1:50; BD, Cat. No. 557831), CD19-APC-Cy7 (1:50; BD, Cat. No. 557791), CD3-BUV395 (1:40; BD, Cat. No. 563546), CD4-BUV496 (1:40; BD, Cat. No. 612936), CD8-PE (1:40; BD, Cat. No. 555367), CXCR5-PE-Cy7 (1:100; BD, Cat. No. 624052), CD25-BV421 (1:40; BD, Cat. No. 562442), CD127-FITC (1:20; BD, Cat. No. 557938). The samples were then washed, resuspended in FACS buffer and analysed on BD LSRFortessa flow cytometer (Data availability—Figshare 10.6084/m9.figshare.23549964).
Krishnananthasivam S., Li H., Bouzeyen R., Shunmuganathan B., Purushotorman K., Liao X., Du F., Friis C.G., Crawshay-Williams F., Boon L.H., Xinlei Q., Chan C.E., Sobota R., Kozma M., Barcelli V., Wang G., Huang H., Floto A., Bifani P., Javid B, & MacAry P.A. (2023). An anti-LpqH human monoclonal antibody from an asymptomatic individual mediates protection against Mycobacterium tuberculosis. NPJ Vaccines, 8, 127.
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2

Comprehensive Lymphocyte Phenotyping by Flow Cytometry

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To assess lymphocytes by flow cytometry, single cell suspensions were prepared and incubated with primary antibody for 30 minutes on ice. After staining for surface proteins, cells were incubated with Propidium Iodide (BD Biosciences, San Jose, CA, USA) for 10 minutes as a live/dead stain. After staining, cells were fixed with 0.6% formalin. The antibodies used were CD4-PE, CD5-PE, CD19-FITC, CD69-FITC, CD86-PE, B220-APC, CD93-BB515, CD279-APC, CXCR5-PECy7 (all BD Biosciences), IgM-FITC (Southern Biotech), IgD-APC-Cy7 (BioLegend, San Diego, CA, USA), CD21-eFlour450, and CD23-PE-Cy7 (eBioscience Inc., San Diego, CA, USA). Apoptosis was analyzed with Telford reagent. Flow cytometry was performed with a BD LSRII Flow Cytometer and analyzed with FACSDiva software (BD Biosciences, v.8.0).
Tabor D.E, & Gould K.A. (2016). Estrogen Receptor Alpha Promotes Lupus in (NZB x NZW)F1 Mice in a B cell Intrinsic Manner. Clinical immunology (Orlando, Fla.), 174, 41-52.
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3

Comprehensive B and T Cell Profiling

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Donor PBMCs was stained with live-dead marker (1:1000; Invitrogen, LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit) to gate out the dead cells for 20 min in 4 °C. After washing with FACS buffer, the cells were stained with respective B, T cell marker panel antibodies diluted in FACS buffer (1xPBS, 2% FBS, 0.1% sodium azide) at 4 °C for 30 min. B cell panel: CD19-BV510 (1:40; Biolegend, Cat. No. 363020), CD14-APC-Cy7 (1:50; BD, Cat. No. 557831), CD3-APC-Cy7 (1:125; Biolegend, Cat. No. 317342), CD10-PE-Dazzle594 (1:50; Biolegend, Cat. No. 312228), CD21-APC (1:50; Biolegend, Cat. No. 354906), CD27-BV650 (1:40; Biolegend, Cat. No. 302828), CD38-PE-Cy7 (1:40; Biolegend, Cat. No. 356608), PD-1-BV711 (1:20; Biolegend, Cat. No. 329928), IgD-BUV 737 (1:40; BD, Cat. No. 612798), IgG-PerCP (1:40; BD), IgA-PE (1:40; Miltenyi, Cat. No. 130-114-002). T cell panel: CD14-APC-Cy7 (1:50; BD, Cat. No. 557831), CD19-APC-Cy7 (1:50; BD, Cat. No. 557791), CD3-BUV395 (1:40; BD, Cat. No. 563546), CD4-BUV496 (1:40; BD, Cat. No. 612936), CD8-PE (1:40; BD, Cat. No. 555367), CXCR5-PE-Cy7 (1:100; BD, Cat. No. 624052), CD25-BV421 (1:40; BD, Cat. No. 562442), CD127-FITC (1:20; BD, Cat. No. 557938). The samples were then washed, resuspended in FACS buffer and analysed on BD LSRFortessa flow cytometer (Data availability—Figshare 10.6084/m9.figshare.23549964).
Krishnananthasivam S., Li H., Bouzeyen R., Shunmuganathan B., Purushotorman K., Liao X., Du F., Friis C.G., Crawshay-Williams F., Boon L.H., Xinlei Q., Chan C.E., Sobota R., Kozma M., Barcelli V., Wang G., Huang H., Floto A., Bifani P., Javid B, & MacAry P.A. (2023). An anti-LpqH human monoclonal antibody from an asymptomatic individual mediates protection against Mycobacterium tuberculosis. NPJ Vaccines, 8, 127.
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4

Comprehensive B and T Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Donor PBMCs was stained with live-dead marker (1:1000; Invitrogen, LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit) to gate out the dead cells for 20 min in 4 °C. After washing with FACS buffer, the cells were stained with respective B, T cell marker panel antibodies diluted in FACS buffer (1xPBS, 2% FBS, 0.1% sodium azide) at 4 °C for 30 min. B cell panel: CD19-BV510 (1:40; Biolegend, Cat. No. 363020), CD14-APC-Cy7 (1:50; BD, Cat. No. 557831), CD3-APC-Cy7 (1:125; Biolegend, Cat. No. 317342), CD10-PE-Dazzle594 (1:50; Biolegend, Cat. No. 312228), CD21-APC (1:50; Biolegend, Cat. No. 354906), CD27-BV650 (1:40; Biolegend, Cat. No. 302828), CD38-PE-Cy7 (1:40; Biolegend, Cat. No. 356608), PD-1-BV711 (1:20; Biolegend, Cat. No. 329928), IgD-BUV 737 (1:40; BD, Cat. No. 612798), IgG-PerCP (1:40; BD), IgA-PE (1:40; Miltenyi, Cat. No. 130-114-002). T cell panel: CD14-APC-Cy7 (1:50; BD, Cat. No. 557831), CD19-APC-Cy7 (1:50; BD, Cat. No. 557791), CD3-BUV395 (1:40; BD, Cat. No. 563546), CD4-BUV496 (1:40; BD, Cat. No. 612936), CD8-PE (1:40; BD, Cat. No. 555367), CXCR5-PE-Cy7 (1:100; BD, Cat. No. 624052), CD25-BV421 (1:40; BD, Cat. No. 562442), CD127-FITC (1:20; BD, Cat. No. 557938). The samples were then washed, resuspended in FACS buffer and analysed on BD LSRFortessa flow cytometer (Data availability—Figshare 10.6084/m9.figshare.23549964).
Krishnananthasivam S., Li H., Bouzeyen R., Shunmuganathan B., Purushotorman K., Liao X., Du F., Friis C.G., Crawshay-Williams F., Boon L.H., Xinlei Q., Chan C.E., Sobota R., Kozma M., Barcelli V., Wang G., Huang H., Floto A., Bifani P., Javid B, & MacAry P.A. (2023). An anti-LpqH human monoclonal antibody from an asymptomatic individual mediates protection against Mycobacterium tuberculosis. NPJ Vaccines, 8, 127.
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