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Mca2489

Manufactured by Bio-Rad
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The MCA2489 is a laboratory equipment product manufactured by Bio-Rad. It is designed to perform a core function, but a detailed description while maintaining an unbiased and factual approach is not available.

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Lab products found in correlation

A3562, by Merck Group (2 mentions)

2 protocols using mca2489

1

CsgC Binding Assay on Nitrocellulose

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Two µL samples were spotted on a nitrocellulose membrane and air dried. Membrane blocking for non-specific binding was carried out with a 10% (w/v) skimmed milk solution in PBS for 10 min. CsgC was added (0.4 mM in 0.1% (w/v) milk in PBS) for 1h shaking at 37°C to allow binding. Bound CsgC was detected using a mouse anti-strep monoclonal antibody (AbD Serotec; MCA2489; 1:1000 dilution) as primary and an anti-mouse IgG alkaline phosphatase conjugated (Sigma-Aldrich; A3562; 1:5000 dilution) as secondary antibody.
Sleutel M., Van den Broeck I., Van Gerven N., Feuillie C., Jonckheere W., Valotteau C., Dufrêne Y.F, & Remaut H. (2017). Nucleation and growth of a bacterial functional amyloid at single fiber resolution. Nature chemical biology, 13(8), 902-908.
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2

CsgC Binding Assay on Nitrocellulose

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two µL samples were spotted on a nitrocellulose membrane and air dried. Membrane blocking for non-specific binding was carried out with a 10% (w/v) skimmed milk solution in PBS for 10 min. CsgC was added (0.4 mM in 0.1% (w/v) milk in PBS) for 1h shaking at 37°C to allow binding. Bound CsgC was detected using a mouse anti-strep monoclonal antibody (AbD Serotec; MCA2489; 1:1000 dilution) as primary and an anti-mouse IgG alkaline phosphatase conjugated (Sigma-Aldrich; A3562; 1:5000 dilution) as secondary antibody.
Sleutel M., Van den Broeck I., Van Gerven N., Feuillie C., Jonckheere W., Valotteau C., Dufrêne Y.F, & Remaut H. (2017). Nucleation and growth of a bacterial functional amyloid at single fiber resolution. Nature chemical biology, 13(8), 902-908.
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