R8-RGD peptides with a terminal cysteine modification [Cys-RRRRRRRR-c(RGDfK)] were purchased from Chinapeptides Co. Ltd. (Shanghai, China). PEI 25K was purchased from Sigma-aldrich and PEI 1.8K was purchased from Alfa Aesar. YOYO-1, Lyso-Tracker red, Hoechst 33342, Lipofectamine 2000 and Lipofectamine 3000 were purchased from Invitrogen (USA). pUNO1-hTRAILa (hTRAIL) and pUNO1-MCS (MCS) plasmid were obtained from In vivo Gen (San Diego, CA, USA) and purified with QIAGEN Plasmid Mega Kit (Qiagen GmbH, Hilden, Germany). Annexin V-FITC /PI apoptosis detection kit was purchased from Nanjing KeyGen Biotech. Co., Ltd (Nanjing,China). Antibodies used for western blotting were purchased from Cell Signaling Technology (CST, Beverly, MA, USA).
SW 480 cell line was obtained from American Type Culture Collection (ATCC, USA) and cultured in DMEM with 10% fetal bovine serum (Gibco), 100 units/mL penicillin G sodium and 100 μg/mL streptomycin sulfate and maintained at 37 °C in a humidified and 5% CO2 incubator.
SW 480 cell line was obtained from American Type Culture Collection (ATCC, USA) and cultured in DMEM with 10% fetal bovine serum (Gibco), 100 units/mL penicillin G sodium and 100 μg/mL streptomycin sulfate and maintained at 37 °C in a humidified and 5% CO2 incubator.