Pan γδtcr pe clone immu510

Manufactured by Beckman Coulter

Sourced in United Kingdom

Pan-γδTCR-PE (clone IMMU510) is a monoclonal antibody conjugated with the fluorescent dye R-Phycoerythrin (PE). It is designed to detect the gamma-delta T cell receptor (γδTCR) on the surface of human cells.

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Lab products found in correlation

Facsdiva software, by BD (3 mentions) Lsrfortessa, by BD (3 mentions) Hucd45 pb clone hi30, by Sony (2 mentions) Mcd45 apc clone 30 f11, by Sony (2 mentions) αβtcr fitc clone ip26, by BioLegend (2 mentions) Cd8 percpcy5.5 clone rpa t8, by BioLegend (2 mentions) Fixable viability dye efluor 506, by Thermo Fisher Scientific (2 mentions) Pd 1 bv711 clone eh12.2h7, by BioLegend (1 mentions) Cd4 pe cy7 clone rpa t4, by BioLegend (1 mentions) Cd45ro pe cy7 uchl 1, by BD (1 mentions) Cd45 fitc 2d1, by BD (1 mentions) Cd3 efluor450 okt3, by Thermo Fisher Scientific (1 mentions) Cd16 fitc 3g8, by BD (1 mentions) Cd4 v450 clone rpa t4, by BD (1 mentions) Cd27 apc efluor780, by Thermo Fisher Scientific (1 mentions) Vδ1 fitc clone ts8, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

IMMU510 (5 citations)

3 protocols using pan γδtcr pe clone immu510

Multiparameter Flow Cytometry Analysis

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The following antibodies were used for flow cytometry analysis: huCD45-PB (clone HI30; Sony Biotechnology), pan-γδTCR-PE (clone IMMU510; Beckman-Coulter), mCD45-APC (clone 30-F11, Sony Biotechnology), αβTCR-FITC (clone IP26; Biolegend), CD4-PeCy7 (clone RPA-T4, Biolegend), CD8-PerCPCy5.5 (clone RPA-T8, Biolegend), PD-1-BV711 (clone EH12.2H7, Biolegend), and TIM3-BV650 (clone F38-2E2, Biolegend). To exclude non-viable cells from the analysis, Fixable Viability Dye eFluor506 was used (eBioscience). All samples were analyzed on a BD LSRFortessa using FACSDiva Software (BD Biosciences).

Johanna I., Hernández-López P., Heijhuurs S., Scheper W., Bongiovanni L., de Bruin A., Beringer D.X., Oostvogels R., Straetemans T., Sebestyen Z, & Kuball J. (2021). Adding Help to an HLA-A*24:02 Tumor-Reactive γδTCR Increases Tumor Control. Frontiers in Immunology, 12, 752699.

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Flow Cytometry Antibody Panel

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Antibodies used for flow cytometry include: pan-γδTCR-PE (clone IMMU510; Beckman Coulter, Woerden, the Netherlands), pan-αβTCR-APC (clone IP26; eBioscience, Thermo Fisher Scientific), CD4-V450 (clone RPA-T4; BD Biosciences), CD8α-PerCP-Cy5.5 (RPA-T8; Biolegend), CD3-eFluor 450 (OKT-3; eBioscience), CD45-FITC (2D1; BD Biosciences), CD16-FITC (3G8; BD Biosciences), CD56-FITC (MY31; BD Biosciences), CD27-APC-eFluor780 (O323; eBioscience), CD45RO-PE-Cy7 (UCHL-1; BD Biosciences). All samples were analyzed on a BD LSRFortessa using FACSdiva software (BD Biosciences).

Straetemans T., Kierkels G.J., Doorn R., Jansen K., Heijhuurs S., dos Santos J.M., van Muyden A.D., Vie H., Clemenceau B., Raymakers R., de Witte M., Sebestyén Z, & Kuball J. (2018). GMP-Grade Manufacturing of T Cells Engineered to Express a Defined γδTCR. Frontiers in Immunology, 9, 1062.

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Multiparametric Flow Cytometry Analysis

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The following antibodies were used for flow cytometry analysis: huCD45-PB (clone HI30; Sony Biotechnology, Surrey, United Kingdom), mCD45-APC (clone 30-F11, Sony Biotechnology), αβTCR-FITC (clone IP26; Biolegend, London, United Kingdom), pan-γδTCR-PE (clone IMMU510; Beckman Coulter, Woerden, The Netherlands), CD8-PerCPCy5.5 (clone RPA-T8, Biolegend), CD4-PeCy7 (clone TPA-R4, Biolegend), and Vδ1-FITC (clone TS8.2, Thermo Fisher Scientific, Breda, The Netherlands). To exclude nonviable cells from the analysis, Fixable Viability Dye eFluor506 was used (eBioscience, Thermo Fisher Scientific). All samples were analyzed on a BD LSRFortessa using FACS-Diva Software (BD Biosciences).

Johanna I., Hernández-López P., Heijhuurs S., Bongiovanni L., de Bruin A., Beringer D., van Dooremalen S., Shultz L.D., Ishikawa F., Sebestyen Z., Straetemans T, & Kuball J. (2020). TEG011 persistence averts extramedullary tumor growth without exerting off-target toxicity against healthy tissues in a humanized HLA-A*24:02 transgenic mice. Journal of leukocyte biology, 107(6), 1069-1079.

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