Cell count represents the total number of nucleated cells aspirated from the bone marrow and injected into the shoulder joint. Cell count data were accessed from a laboratory database (Centeno Schultz Clinic, Broomfield, CO, USA). Data were available for only one clinic. For cell counting, 5 μL samples were obtained and red blood cells were lysed in 995 μL of sterile distilled water (Thermo Fisher Scientific, Waltham, MA, USA). The number of cells was then manually counted for four times under a microscope (National Optical, Schertz, TX, USA) using a hemocytometer (Reichert Bright-Line; Hausser Scientific, Horsham, PA, USA). The average of the four counts was calculated. The cell count was obtained by multiplying the dilution factor, volume of the hemocytometer, and final volume of the sample.
Reichert bright line
Manufactured by Hausser Scientific
Sourced in United States
The Reichert Bright-Line is a laboratory microscope designed for general-purpose use. It features a bright-field illumination system and a binocular viewing head. The microscope is equipped with objectives that provide various magnification levels, allowing users to observe and examine samples at different levels of detail.
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Lab products found in correlation
2 protocols using reichert bright line
1
Bone Marrow Cell Quantification
2
Enumerating Nucleated Cells in Bone Marrow
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The predictor of interest in this study was the total nucleated cell count contained in the bone marrow aspirate. The number of nucleated cells in each patient sample was manually counted under a microscope (National Optical, Schertz, TX) using a hemocytometer (Reichert Bright-Line, Hausser Scientific, Horsham, PA). Prior to counting, red blood cells were lysed by diluting 5 μL of sample in 995 μL of sterile, distilled water (Life Technologies, Grand Island, NY). Each sample was counted four times and the average calculated. The total nucleated cell count for injection was determined by multiplying the dilution factor, the volume of the hemocytometer, and the final volume of the sample.
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