Cytopainter mitochondrial staining kit red fluorescence

Penicillin–streptomycin 100,000 UI/mL, poly(ethylenimine) (PEI) 1300~50% in H₂O, sodium citrate, cytochrome c from equine heart, transferrin, fibrinogen, human serum albumin, bovine serum albumin, staurosporine from streptomyces, and 3-(4,5-dimethulthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Slide-A-Lyzer^® Dialysis Cassette–2000 MWCO 3.0–12 mL Capacity and DRAQ5™ were purchased from Thermo Scientific, Rockford, IL, USA. l-Valine, l-Serine, l-Methionine, l-Leucine, l-Aspartic Acid, l-Tryptophan, l-Asparagine monohydrate, l-Phenylalanine were purchased from ThermoFischer (Kandel, Germany). Fetal bovine serum and phosphate saline buffer were purchased from Gibco, Grand Island, NY, USA. Etoposide was purchased from Fresenius Kabi, Bad Homburg, Germany. CytoPainter Mitochondrial Staining Kit Red Fluorescence was purchased from Abcam, Cambridge, UK. Phalloidin-FITC Acti-stainTM 488 Fluorescent Phalloidin was purchased from Cytoskeleton Inc., Denver, CO, USA. Prolong^® Gold Antifade Reagent Molecular Probes was purchased from Cell Signaling Technology, Danvers, CO, USA. Double distilled water (18.2 MΩ) was used as a solvent.