Supernatants from cell cultures were collected at the times indicated for each experiment in the figure legends. The following ELISA monoclonal Ab (mAb) pairs were used: anti-mouse IL-17 (clones TC11-18H10/TC11-8H4.1, BD Biosciences) and IFN-γ (AN-18/R4-6A2, BD Biosciences). All antibodies were used at 1.5 μg/mL for capture and detection. The recombinant cytokines used as standards were purchased from Peprotech. Detection antibodies were all biotinylated. Streptavidin-conjugated horseradish peroxidase (HRP) was added and visualized by o-phenylenediamine dihydrochloride (SIGMA) (from tablets) or 3,3', 5,5’-tetramethylbenzidine solution (TMB, KPL). IL-22 was measured using the Quantikine®ELISA mouse/rat IL-22 kit. Supernatants were incubated undiluted or diluted in polystyrene microtiter plates (Nunc), except for IL-22 ELISA where the plate was included in the kit. Absorbances at 490nm or 450nm were measured using a tunable microplate reader (VersaMax, Molecular Devices). Cytokine supernatant concentrations were calculated by extrapolating absorbance values from standard curves where known concentrations were plotted against absorbance using SoftMax Pro 5 software.
Anti mouse il 17 clones tc11 18h10 tc
Manufactured by BD
Anti-mouse IL-17 (clones TC11-18H10/TC) is a laboratory reagent used for the detection and quantification of mouse interleukin-17 (IL-17) in various biological samples. It is a monoclonal antibody that specifically binds to mouse IL-17.
Automatically generated - may contain errors
2 protocols using anti mouse il 17 clones tc11 18h10 tc
1
Quantifying Cytokine Secretion in Cell Cultures
2
Quantifying Cytokine Secretion in Cell Cultures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Supernatants from cell cultures were collected at the times indicated for each experiment in the figure legends. The following ELISA monoclonal Ab (mAb) pairs were used: anti-mouse IL-17 (clones TC11-18H10/TC11-8H4.1, BD Biosciences) and IFN-γ (AN-18/R4-6A2, BD Biosciences). All antibodies were used at 1.5 μg/mL for capture and detection. The recombinant cytokines used as standards were purchased from Peprotech. Detection antibodies were all biotinylated. Streptavidin-conjugated horseradish peroxidase (HRP) was added and visualized by o-phenylenediamine dihydrochloride (SIGMA) (from tablets) or 3,3', 5,5’-tetramethylbenzidine solution (TMB, KPL). IL-22 was measured using the Quantikine®ELISA mouse/rat IL-22 kit. Supernatants were incubated undiluted or diluted in polystyrene microtiter plates (Nunc), except for IL-22 ELISA where the plate was included in the kit. Absorbances at 490nm or 450nm were measured using a tunable microplate reader (VersaMax, Molecular Devices). Cytokine supernatant concentrations were calculated by extrapolating absorbance values from standard curves where known concentrations were plotted against absorbance using SoftMax Pro 5 software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!