Western lighting chemiluminescence reagent

Manufactured by Merck Group

The Western Lighting Chemiluminescence Reagent is a laboratory product designed for the detection and quantification of proteins in Western blot analysis. The reagent produces a chemiluminescent signal when it reacts with the horseradish peroxidase (HRP) enzyme that is commonly used to label antibodies in Western blotting procedures.

Automatically generated - may contain errors

Lab products found in correlation

Cell lysis buffer, by Cell Signaling Technology (1 mentions) Protease inhibitor, by Thermo Fisher Scientific (1 mentions) Anti cyp11a1, by Cell Signaling Technology (1 mentions) Bca assay, by Bio-Rad (1 mentions) Anti actin hrp, by Merck Group (1 mentions) Laemmli sample buffer, by Bio-Rad (1 mentions) Anti β actin antibody, by Merck Group (1 mentions) Mab979, by Merck Group (1 mentions)

Spelling variants of this product

Chemiluminescence reagent (157 citations)

2 protocols using western lighting chemiluminescence reagent

Protein Extraction and Western Blotting for Steroidogenic Enzymes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Platelet protein extracts were prepared using cell lysis buffer (Cell Signaling; cat no. 9803S) with protease inhibitors (Thermo Scientific; cat no. 78410). Protein concentrations were determined by BCA assays (BioRAD). Samples were boiled in Laemmli sample buffer (BioRAD) with β-ME and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. One hundred micrograms of protein was loaded per lane. Proteins were transferred to a nitrocellulose membrane, blocked in 5% nonfat milk (wt/vol in PBS), and immunoblotted with indicated primary antibodies and appropriate horseradish peroxidase–conjugated secondary antibodies. Immunoblots were developed using Western Lighting Chemiluminescence Reagent (Milipore, Billerica, MA).
Antibodies anti-CYP17A1 (cat no. SAB1300941), anti-HSD3B2 (cat no. SAB2101087), and anti–actin-HRP (cat no. A3854) were from Sigma-Aldrich; anti-CYP11A1 (cat no. 12491) was from Cell Signaling Technology (Danvers, MA).

Zaslavsky A.B., Gloeckner-Kalousek A., Adams M., Putluri N., Venghatakrishnan H., Li H., Morgan T.M., Feng F.Y., Tewari M., Sreekumar A, & Palapattu G.S. (2015). Platelet-Synthesized Testosterone in Men with Prostate Cancer Induces Androgen Receptor Signaling. Neoplasia (New York, N.Y.), 17(6), 490-496.

+ Open protocol

+ Expand

Western Blot Analysis of EV71 Capsid Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

MAB979, a mouse monoclonal antibody that specifically recognizes EV71 VP0/VP2 capsid proteins (Millipore), MAB1255-M08, a mouse monoclonal antibody that specifically recognizes EV71 VP1 capsid protein (Abnova), and anti-β-actin antibody (Sigma-Aldrich) were used as primary antibodies. Infected cells or concentrated virus samples were lysed in loading buffer and resolved by electrophoresis in a 12% SDS-PAGE. Proteins were transferred onto a PVDF membrane, blocked with 5% skim milk, and probed with primary antibodies, including anti-EV71 VP0/VP2 antibody (diluted 1:1000), anti-EV71 VP1 antibody (diluted 1:1000), or anti-β-actin antibody (diluted 1:5000) at 4°C overnight. After incubation, the 1:5000 diluted HRP-conjugated secondary antibody was added, followed by incubation for 2 hours at room temperature. After four wash steps with PBS plus 0.1% Tween 20 (PBST), signals were detected using Western Lighting chemiluminescence reagent (Millipore) and the densitometry of specific blots was calculated using the ImageJ software.

Zhang Y.X., Huang Y.M., Li Q.J., Li X.Y., Zhou Y.D., Guo F., Zhou J.M, & Cen S. (2017). A highly conserved amino acid in VP1 regulates maturation of enterovirus 71. PLoS Pathogens, 13(9), e1006625.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!