Media and cell pellet metabolic products were quantified and compared by integrated peak area values under corresponding total ion current (TIC) ID logs as determined by retention times in the selected ion monitoring mode by blinded spectra processing personnel (see Acknowledgments section). Average peak width values were less than 5 s (ChemStation Integrator, Agilent, Palo Alto, CA, USA) using identical injection volume, solvent strength and split ratios with that of natural 13C-labeled external chromatographic standards. Concentration-dependent integrated chromatographic TIC areas are displayed as arbitrary values of metabolite concentration among the control and fructose treated groups.
Chem station integrator
Manufactured by Agilent Technologies
Sourced in United States
The ChemStation integrator is a software package designed for data analysis and instrument control in analytical chemistry applications. It provides basic functions for data acquisition, peak integration, and report generation.
Automatically generated - may contain errors
Lab products found in correlation
Hp 5ms capillary column, by Agilent Technologies (3 mentions)
Agilent 5975c gc ms system, by Agilent Technologies (2 mentions)
G1888 network headspace autosampler, by Agilent Technologies (1 mentions)
Agilent 5975 gc ms system, by Agilent Technologies (1 mentions)
Gc 7890a ms 5975, by Agilent Technologies (1 mentions)
Agilent 7890a, by Agilent Technologies (1 mentions)
9 protocols using chem station integrator
1
Quantifying Metabolic Products by TIC
2
Ethanol Blood Sampling Protocol
3
HPLC Analysis of Cinnamic and Vanillic Acids
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An HPLC analysis of the cinnamic and vanillic acid concentrations in the liquid medium samples from the experiments described in Section 2.3 was carried out according to Gambacorta et al. [12 ] using a HPLC binary system (Agilent, model G1311A, Santa Clara, CA, USA) equipped with a 7725 Rheodyne injector, a 20 μL sample loop, adiode array detector (Agilent, model G1315Bm), and a Chem Station integrator (Agilent) for data acquisition. The stationary phase was a Nova-Pack C18 analytical column (150 mm length, 3.9 mm i.d.) with a particle size of 4 μm (Waters, Milford, MA, USA). The mobile phases for chromatographic analysis were (A) water:acetic acid (98:2, v/v) and (B) methanol:acetonitrile (1:1, v/v) at a constant flow rate of 1 mL/min. The gradient program of solvent was as follows: 0 to 30 min 100% A; 30 to 45 min 70% A; 45 to 55 min 50% A; 55 to 65 min 40% A; 65 to 75 min 0% A. The compound identification was carried out by comparing the peak retention times with those obtained by the injection of pure standards. The concentrations were expressed as mg/L.
The experiments were performed at least over two independent batches. The results were analyzed as a decrease in phenols after 21 days and submitted to ANOVA and MANOVA.
The experiments were performed at least over two independent batches. The results were analyzed as a decrease in phenols after 21 days and submitted to ANOVA and MANOVA.
4
GC-MS Analysis of Biofuels NOSE and NORE
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NOSE and NORE were initially bi-fractionated by dissolving in dichloromethane and n-hexane separately. The mixtures were centrifuged thrice (12,000 rpm) for 15 min. The clear supernatant was used for GC-MS analysis using Agilent 5975C GC-MS system (Agilent Technologies, USA) attached with HP-5 ms Capillary Column (30 m × 0.25 mm i.d. × 0.25 μm film thickness). The machine was equipped with inert MSD triple axis mass detector conditioned at ion trap 200 °C, transfer line 280 °C, electron energy 70 eV (vacuum pressure- 2.21e–0.5 torr) was used for analysis. Helium was used as carrier gas (1 ml/min). Sample volume was 2 μl and injected in a splitless mode. The column temperature was kept at 60 °C for 1 min followed by 5 °C/min up to 250 °C. The major and essential compounds present in samples were identified by their retention times and mass fragmentation patterns using Agilent Chem Station Integrator and the database of National Institute Standard and Technology (NIST) with a MS library version 2010.
5
Comparative Flavor Analysis of CPM and Beef
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To evaluate the potential of the CPM as a food product, samples of the CPM and lyophilized beef (tenderloin) were each grilled on a hot-plate with a little oil at 120 °C for 15 min. The flavor analysis was then performed via the headspace-solid phase microextraction (HS-SPME) method of gas chromatography-mass spectrometry (GC-MS, Agilent 8890 GC system-Agilent 5677B MSD, Agilent Technologies). The analytes were separated on a HP-5ms column (30 m × 250 μm × 0.25 μm). The temperature of the oven was initially maintained at 40 °C for 5 min then raised to 160 °C at a rate of 4 °C/min, then increased to 250 °C at a rate of 7 °C/min, and maintained for 10 min. The mass spectra (MS) were acquired in normal scanning mode with a source temperature of 230 °C. The volatile compounds were identified by comparison with the data from the spectral library (Agilent Chemstation Integrator). The flavors were referenced from the Flavor Extract Manufacturers Association of the United States (FEMA) and the joint FAO/WHO Expert Committee on Food Additives (JECFA) lists.
6
GC-MS Analysis of Bioactive Compounds in CBL Extract
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CBL extract was dissolved in n-hexane and the mixture was centrifuged thrice at 12,000 rpm for 15 min for GC-MS analyses. Supernatant was collected and used for GC–MS analysis. Agilent 5975 GCMS system (Agilent Technologies, USA) attached with HP-5 ms Capillary Column (30 m × 0.25 mm i.d. × 0.25 μm film thickness) and equipped with inert MSD triple axis mass detector condition edation trap 200°C, transfer line 280°C, electronenergy70eV (vacuum pressure-2.21e0.5 Torr) was used to identify the bio active compounds present in CBL extract. Helium was used as a carrier gas at a flow rate of 1 ml/min and 2 ml sample was injected in a split less mode. The column temperature was set at 60°C for 1 min followed by 5°C/min up to 250°C and the essential compounds in CBL were identified by the retention times and mass fragmentation patterns using Agilent Chem Station integrator and the database of National Institute of Standard and Technology (NIST) with a MS library version2011.
7
GC-MS Analysis of Lipid Fraction Extract
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LFE was dissolved in n-hexane and the mixture was centrifuged thrice at 12,000 rpm for 15 min. The clear supernatant was used for GC–MS analysis. Agilent 5975 CGCMS system (Agilent Technologies, USA) attached with HP-5 ms Capillary Column (30 m × 0.25 mm i.d. × 0.25 μm film thickness) and equipped with inert MSD triple axis mass detector condition edation trap 200 °C, transfer line 280 °C, electronenergy70eV (vacuum pressure-2.21e-0.5 Torr) was used for analysis. The carrier gas, helium, was used at a flow rate of 1 ml/min. 2 ml sample was injected in a split less mode. The column temperature was set at 60 °C for 1 min followed by 5 °C/min up to 250 °C. The major and essential compounds in LFE were identified by the retention times and mass fragmentation patterns using Agilent Chem Station integrator and the database of National Institute of Standard and Technology (NIST) with a MS library version2011.
8
GC-MS Analysis of Plant Extracts
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The shadow-dried powder of the two samples (120 gm of root and 150 gm of flower) was exposed to hydro-distillation for 4 h by a Clevenger device. The obtained oil was again dried with anhydrous sodium sulfate and then later kept at 4°C.
GC-MS was carried out by Agilent GC (7890A)/MS (5975) with a capillary column, He gas carrier with a flow rate of 1.5 ml/min, flame ionization detector, and a split ratio of 1:25. The temperature was set at 50 °C for the column for 1 min and then for 265 °C for the heating purpose at the rate of 2.5°C/min, then stored at 265 °C for 20min. detector temp at 300 °C, injector temp at 265 °C flow of Helium at 35 ml/min with the airflow rate of 400 ml/min. The MS set off at 70 eV ionization energy. Retention times have been recorded and calculated by utilizing the n-alkanes retention times which have been injected in the same condition after the oil (www.agilent.com ). The final found chemical compounds were reported by the “Agilent’s ChemStation Integrator” algorithm (www.agilent.com ).
GC-MS was carried out by Agilent GC (7890A)/MS (5975) with a capillary column, He gas carrier with a flow rate of 1.5 ml/min, flame ionization detector, and a split ratio of 1:25. The temperature was set at 50 °C for the column for 1 min and then for 265 °C for the heating purpose at the rate of 2.5°C/min, then stored at 265 °C for 20min. detector temp at 300 °C, injector temp at 265 °C flow of Helium at 35 ml/min with the airflow rate of 400 ml/min. The MS set off at 70 eV ionization energy. Retention times have been recorded and calculated by utilizing the n-alkanes retention times which have been injected in the same condition after the oil (
9
GC-MS Analysis of Organic Compounds
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Agilent 7890A (Agilent, Santa Clara, CA, USA) was used to carry out the GC-MS analysis. GC was equipped with a DB-5 (30 m × 0.25 mm × 0.25 μm) capillary column (Agilent, Santa Clara, CA, USA). Initially, the instrument was maintained at a temperature of 100 °C for 2.1 min. The temperature rose to 300 °C at a rate of 25 °C/min and was maintained for 20 min. The injection port temperature and helium flow rate were sustained at 250 °C and 1.5 mL/min, respectively. The ionization voltage was 70 eV. The samples were injected in the split mode at 10:1. The MS scan range was set at 35–900 (m/z). The fragmentation patterns of mass spectra were compared with those stored in the W8N05ST Library MS database. The percentage of each compound was calculated from the relative peak area of each compound in the chromatogram. The concept of integration was used with the ChemStation integrator (Agilent, Santa Clara, CA, USA) algorithms (analyzed 19 May 2021) [84 (link)].
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