All mouse strains were bred and housed in specific pathogen-free conditions in accordance with the Institutional Animal Care and Use Guidelines of the University of California, San Diego (UCSD) at a temperature between 18 °C and 23 °C with 40–60% humidity. Male and female mice were both used in the present study. All mice used were on a C57BL/6J background. P14, Tgfbr2fl/fl mice (stock no. 012603, Jackson Laboratory), R26Cre-ERT2 (stock no. 008463, Jackson Laboratory), Thy1.1 and CD45.1 congenic mice were bred in house. Prdm1fl/fl (stock no. 008100, Jackson Laboratory) and Gzmb-cre (stock no. 003734, Jackson Laboratory) spleens were a gift from the laboratory of S. Kaech. To delete floxed alleles using Cre-ERT2, we administered 1 mg of tamoxifen (Cayman Chemical Company) emulsified in 100 μl of sunflower seed oil (Sigma-Aldrich) via daily intraperitoneal injections on days 14–18 of infection. All animal studies were approved by the Institutional Animal Care and Use Committees of UCSD and performed in accordance with UC guidelines.
Prdm1fl fl
Manufactured by Jackson ImmunoResearch
Prdm1fl/fl is a lab equipment product that functions as a genetic construct used for conditional gene targeting. It contains loxP sites flanking the Prdm1 gene, allowing for cell-type-specific deletion of the gene when used in combination with Cre recombinase.
Automatically generated - may contain errors
Lab products found in correlation
Sunflower seed oil, by Merck Group (1 mentions)
R26creert2, by Jackson ImmunoResearch (1 mentions)
Tgfbr2fl fl mice, by Jackson ImmunoResearch (1 mentions)
Tamoxifen, by Cayman Chemical (1 mentions)
Titermax gold adjuvant, by Merck Group (1 mentions)
Wt c57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
Cd19 cre, by Jackson ImmunoResearch (1 mentions)
Il 6 ko, by Jackson ImmunoResearch (1 mentions)
2 protocols using prdm1fl fl
1
Genetic Manipulation of Murine Immune Cells
2
Topo I-Induced Autoimmune Model in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
WT C57BL/6 mice, IL-6KO, IL-23KO, CD19-Cre, PRDM1 fl/fl, or MHC class II fl/fl mice were purchased from The Jackson Laboratory (Bar Harbor, ME) or Taconic Biosciences (Rensselaer, NY). These mice were backcrossed between 10 generations onto the C57BL/6 genetic background. All mice were housed in a specific pathogen-free barrier facility and screened regularly for pathogens. The mice used in these experiments were 6 weeks of age. Recombinant topo I proteins were purified from Escherichia coli expressing human topo I and were dissolved in saline (100 μg/ml). The topo I solution was mixed 1:1 (volume/volume) with TiterMax Gold adjuvant (Sigma-Aldrich, St. Louis, MO). These solutions (100 μl) were injected up to four times subcutaneously into a single location on the shaved back of the mice with a 26-gauge needle at an interval of 2 weeks, as described previously (Yoshizaki et al., 2011 (link); Fukasawa et al., 2017 (link)). In the mouse studies, at least six mice per group were examined. All studies and procedures were approved by the Committee on Animal Experimentation of the University of Tokyo Graduate School of Medicine.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!