Glycidyl methacrylate

All chemicals used for magnetic sorbent synthesis were analytical grade products and were used as received. Glycidyl methacrylate (GMA), ethylene glycol dimethacrylate (EGDMA), diethylenetriamine (deta), 2,2′-Azobis(2-methylpropionitrile) (AIBN), cyclohexanol, and 1-tetradecanol were obtained from Merck (Darmstadt, Germany). Magnetite (nanopowder with particle size 50 nm, ≥98% trace metals basis) and APTMS were obtained from Sigma-Aldrich (Taufkirchen, Germany). Poly(N-vinyl pyrrolidone) (PVP, Kollidone 90) was purchased from BASF (Ludwigshafen, Germany). Toluene, methanol, and ethanol were obtained from Zorka Pharma (Šabac, Serbia). Potassium dichromate (K₂Cr₂O₇, p.a. purity) was obtained from Merck (Darmstadt, Germany). Deionized water was used for the preparation of the solution.

GC (≥60% calculated by titration, crystalline, MW ≈ 585,000 g/mol) and glycidyl methacrylate (GM) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Riboflavin 5′-monophosphate sodium salt (riboflavin; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) was used for photo-curing. Doxorubicin·hydrochloride (DOX·HCl; Tokyo Chemical Industry Co., Ltd., Tokyo, Japan) and cisplatin (CP; Sigma-Aldrich, St. Louis, MO, USA) were used for OSA therapy. (2-hydroxypropyl)-beta-cyclodextrin (HP-β-CD; St. Louis, MO, USA) was used to improve the water solubility of CP. Cellulose membrane (cut-off: 20 kDa) was purchased from Spectrum Laboratories Inc. (Rancho Dominguez, CA, USA). MG-63 and KHOS/NP human osteosarcoma cell lines were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA). A cell counting kit-8 (CCK-8; Dojindo Molecular Technologies, Inc. Rockville, MD, USA) was used for in vitro cell viability assay. All chemicals were used as received.

Glycol chitosan (GC) and glycidyl methacrylate (GM) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bone morphogenetic protein-2 (BMP-2) and transforming growth factor-beta1 (TGF-β1) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) was obtained from Wako Pure Chemical Industries (Osaka, Japan). Dialysis tubes (Spectrum Laboratories Inc., Rancho Dominguez, CA, USA) were used for purification. The MC3T3-E1 cell line was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). α-Minimum Eagle’s medium, fetal bovine serum (FBS), and penicillin and streptomycin (PS) were supplied by Life Technologies (Grand Island, NY, USA). All chemicals were used as received.

GC (≥60% calculated by titration, crystalline, MW ≈ 585,000 g/mol) and glycidyl methacrylate (GM) for visible-light-cured hydrogel preparation were purchased from Sigma-Aldrich (St. Louis, MO, USA). Riboflavin 5′-monophosphate sodium salt (riboflavin) used for photo curing was supplied by Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Doxorubicin hydrochloride (DOX⋅HCl; Tokyo Chemical Industry Co., Ltd, Tokyo, Japan) and paclitaxel (PTX; Shin Poong Pharm, Co., Ltd.; Ansan, Kyunggi, Republic of Korea) were used for breast cancer therapy. β-CD (Sigma-Aldrich; St. Louis, MO, USA) was used to improve the water solubility of PTX. The cellulose membrane was purchased from Spectrum Laboratories Inc. (Rancho Dominguez, CA, USA) and theMCF-7 human breast cancer cell line was obtained from American Type Culture Collection (ATCC; Manassas, VA, USA). Cell counting kit-8 (CCK-8; Dojindo Molecular Technologies, Inc. Rockville, MD, USA) was used for the in vitro cell viability assay. All chemicals were used as received.

The following chemicals
were purchased from
Sigma-Aldrich: ammonia solution (NH₄OH, 25%), ethyl ether
(≥99%), hexadecyl trimethylammonium bromide (CTAB, ≥98%),
tetraethylorthosilicate (TEOS, ≥99.99%), (3-aminopropyl)-triethoxysilane
(APTES, ≥98%), bromoacetyl bromide (≥98%), triethylamine
(≥99%), 2-bromoisobutyryl bromide (BIBB, 98%), N-isopropylacrylamide (NIPAm, ≥99%), glycidyl methacrylate
(GMA, ≥97%), tris(2-dimethylaminoethyl)amine (Me₆TREN, 96%), copper(I) bromide (99.99%), copper(II) bromide (99%),
Alizarin Red S (ARS, grade certified by the Biological Stain Commission),
copper(II) sulfate (≥99%), sodium ascorbate, sodium azide (≥99.5%),
bovine serum albumin (BSA, ≥98%), ovalbumin from chicken egg
white (OVA, ≥98%), methanol (≥99.9%), tetrahydrofuran
(THF, ≥98%), isopropanol (99.5%), N,N-dimethylformamide (DMF, 99.8%), β-nicotinamide adenine
dinucleotide, reduced disodium salt hydrate (NADH, ≥97%), acetic
acid (≥99%), hydrochloric acid (37%), and lactate dehydrogenase
(LDH) from bovine heart (type III, crystalline suspended in ammonium
sulfate solution). Ethanol (99.5%) was purchased from Solveco. Prior
to use, CuBr was stirred in acetic acid for 24 h, collected by centrifugation,
washed with water and methanol, and dried in a vacuum desiccator.
3-(Prop-2-ynyloxycarbonylamino)phenylboronic acid (PCAPBA) was synthesized
by use of a literature method.^{22 (link)}