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Cx3cr1cre

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Cx3cr1Cre is a gene-targeted mouse line that expresses Cre recombinase under the control of the Cx3cr1 promoter. Cx3cr1 is a chemokine receptor expressed in cells of the monocyte/macrophage lineage, including microglia in the central nervous system.

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Lab products found in correlation

Lyz2 cre, by Jackson ImmunoResearch (4 mentions) C57bl 6j, by Jackson ImmunoResearch (3 mentions) B6.129p2 lyz2tm1 cre, by Jackson ImmunoResearch (2 mentions) Sting golden ticket stinggt gt mouse, by Jackson ImmunoResearch (2 mentions) C57bl 6, by Jackson ImmunoResearch (2 mentions) Mrp8 cre, by Jackson ImmunoResearch (2 mentions) Dba 1j, by Jackson ImmunoResearch (2 mentions) Ubc creert2, by Jackson ImmunoResearch (2 mentions) Zbtb46cre, by Jackson ImmunoResearch (1 mentions) E8icre, by Jackson ImmunoResearch (1 mentions) Lysm cre, by Jackson ImmunoResearch (1 mentions) Cd11c cre, by Jackson ImmunoResearch (1 mentions) Cd4 cre, by Jackson ImmunoResearch (1 mentions) Cx3cr1cre mice, by Jackson ImmunoResearch (1 mentions) Tamoxifen, by Merck Group (1 mentions) C57bl 6 mice, by Jackson ImmunoResearch (1 mentions) Scid mice, by Jackson ImmunoResearch (1 mentions) Gfap cre, by Jackson ImmunoResearch (1 mentions) Rpl22 ha, by Jackson ImmunoResearch (1 mentions) C57bl 6j mice, by Daehan Biolink (1 mentions)

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Cx3cr1Cre mice (8 citations) CX3CR1Cre/ERT2 mice (4 citations)

10 protocols using cx3cr1cre

1

Characterization of C9orf72 and STING Mice

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All mice were housed in pathogen-free facilities under 12-h light dark cycles with access to food and water ad libitum. Temperature was set 74°F +/− 2° with humidity 30–70%. C9orf72−/− mice were as previously published7 (link) and wildtype mice were purchased from Jackson Laboratories. C9orf72fl/fl mice were provided by the Pasterkamp lab12 (link) and were crossed with Cx3cr1Cre (B6J.B6N(Cg)-Cx3cr1tm1.1(cre)Jung/J) and Lyz2Cre (B6.129P2-Lyz2tm1(cre)Ifo/J) purchased from Jackson Laboratories to obtain Cx3cr1Cre;C9orf72fl/fl and Lyz2Cre;C9orf72fl/fl mice. The STING golden-ticket (STINGgt/gt) mouse was obtained from Jackson Laboratories (C57BL/6J- Tmem173gt/J). C9orf72−/− mice were crossed with STINGgt/gt to obtain C9orf72−/−;STINGgt/gt mice. All mice have the nuclear background of C57BL/6J. Mice were sex- and age-matched. For all experiments mice were grouped according to genotype. For EAE and B16 melanoma models genotypes were randomly separated into experimental groups. Researchers were blinded when scoring EAE, counting tumor burden in B16 melanoma model. Husbandry and behavioral tests were conducted in accordance with the protocols described by the National Institutes of Health’s Guide for the Care and Use of Animals and were approved by Cedars-Sinai Institutional Animal Care and Use Committees (IACUC #8161).
McCauley M.E., O’Rourke J.G., Yáñez A., Markman J.L., Ho R., Wang X., Chen S., Lall D., Jin M., Muhammad A.K., Bell S., Landeros J., Valencia V., Harms M., Arditi M., Jefferies C, & Baloh R.H. (2020). C9orf72 in myeloid cells suppresses STING-induced inflammation. Nature, 585(7823), 96-101.
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2

Characterization of C9orf72 and STING Mice

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All mice were housed in pathogen-free facilities under 12-h light dark cycles with access to food and water ad libitum. Temperature was set 74°F +/− 2° with humidity 30–70%. C9orf72−/− mice were as previously published7 (link) and wildtype mice were purchased from Jackson Laboratories. C9orf72fl/fl mice were provided by the Pasterkamp lab12 (link) and were crossed with Cx3cr1Cre (B6J.B6N(Cg)-Cx3cr1tm1.1(cre)Jung/J) and Lyz2Cre (B6.129P2-Lyz2tm1(cre)Ifo/J) purchased from Jackson Laboratories to obtain Cx3cr1Cre;C9orf72fl/fl and Lyz2Cre;C9orf72fl/fl mice. The STING golden-ticket (STINGgt/gt) mouse was obtained from Jackson Laboratories (C57BL/6J- Tmem173gt/J). C9orf72−/− mice were crossed with STINGgt/gt to obtain C9orf72−/−;STINGgt/gt mice. All mice have the nuclear background of C57BL/6J. Mice were sex- and age-matched. For all experiments mice were grouped according to genotype. For EAE and B16 melanoma models genotypes were randomly separated into experimental groups. Researchers were blinded when scoring EAE, counting tumor burden in B16 melanoma model. Husbandry and behavioral tests were conducted in accordance with the protocols described by the National Institutes of Health’s Guide for the Care and Use of Animals and were approved by Cedars-Sinai Institutional Animal Care and Use Committees (IACUC #8161).
McCauley M.E., O’Rourke J.G., Yáñez A., Markman J.L., Ho R., Wang X., Chen S., Lall D., Jin M., Muhammad A.K., Bell S., Landeros J., Valencia V., Harms M., Arditi M., Jefferies C, & Baloh R.H. (2020). C9orf72 in myeloid cells suppresses STING-induced inflammation. Nature, 585(7823), 96-101.
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3

TIM-3 Conditional Knockout Mouse Model

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Six-to eight-week-old C57BL/6, Cd11ccre, Zbtb46cre, Cd4cre, E8icre, Lysmcre, Cx3cr1cre and Foxp3-ERT2cre mice were purchased from the Jackson Laboratory. Havcr2fl/fl mice were generated as described in supplementary materials. TIM-3 conditional knockout mice were generated by crossing to the above cre lines. For knockin/knockout alleles (Lysmcre) the appropriate controls were used; that is, Havcr2fl/+ × Lysmcre+/−, for all other cre lines fl/fl mice were used as controls. For experiments with Foxp3-ERT2cre mice, mice were orally gavaged with 8 mg tamoxifen 3 days before tumour implantation and every 3 days thereafter for the duration of the experiments. Havcr2+/+Foxp3-ERT2cre were used as an additional wild-type control but results were comparable to Havcr2fl/fl and were therefore not included. Deletion efficiency was determined by flow cytometry (not shown). Animal experiments were done in accordance with the guidelines of the institutional Animal Care and Use Committee (IACUC) at Brigham and Women’s Hospital and Harvard Medical School. All animals were euthanized before reaching humane endpoint, with tumour growth no greater than 2 cm in any one direction or of a total of 400 mm2 overall. Mice of both sexes were used throughout the study; sex-matched and age-matched (8–12 weeks) controls were used in individual experiments.
Dixon K.O., Tabaka M., Schramm M.A., Xiao S., Tang R., Dionne D., Anderson A.C., Rozenblatt-Rosen O., Regev A, & Kuchroo V.K. (2021). TIM-3 restrains anti-tumour immunity by regulating inflammasome activation. Nature, 595(7865), 101-106.
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4

Conditional P2Y6 Receptor Deletion in Mice

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C57BL/6 mice were purchased from the Jackson Laboratory and Charles River. P2ry6fl/fl/+/+ (+/+) mice and P2ry6fl/fl/Cre/+ (Cre/+) mice were generated as previously reported and backcrossed for 10 generations with C57BL/6 mice (29 ). To delete the P2Y6 receptor, we used the ubiquitous inducible Rosa26 Cre recombinase in 8- to 16- week-old P2ry6fl/fl/cre/+ mice by daily intraperitoneal (i.p.) injection of tamoxifen (1mg; MilliporeSigma) for 5 days, beginning 14 days before the first Df treatment, or bone marrow harvesting. Nfatc1fl/fl mice (32 (link)) were provided by Antonios O. Aliprantis and intercrossed with CX3CR1-cre mice (Jackson Laboratory, JAX stock #025524) (33 (link)) to generate Nfatc1fl/fl/CX3CR1cre/+ mice. Nfatc2−/− mice were provided by Anjana Rao and Patrick Hogan (La Jolla Institute, La Jolla)(34 (link)). All mice were housed in groups of two to five mice per cage with a standard 12-hour light/dark cycle and provided food and water ad libitum. All experiments were in accordance with review and approval by the Animal Care and Use Committee of Brigham and Women’s Hospital.
Nagai J., Lin J, & Boyce J.A. (2022). Macrophage P2Y6 Receptor Signaling Selectively Activates NFATC2 and Suppresses Allergic Lung Inflammation. The Journal of Immunology Author Choice, 209(12), 2293-2303.
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5

Conditional Elmo1 Knockout Mice

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C57BL/6J, DBA/1J, NOD, Mrp8-Cre, Cx3cr1-Cre, Lyz2-Cre, and Ubc-CreERt2 mice were obtained from Jackson Laboratories. Elmo1fl/fl and Elmo1–/– mice have been described previously24 (link). To generate mice with deletion of Elmo1 in myeloid cells, neutrophils or macrophages, Elmo1fl/fl mice were crossed to Lyz2-Cre, Mrp8-Cre or Cx3cr1-Cre mice, respectively. To generate mice with inducible deletion of Elmo1, Elmo1fl/fl mice were crossed to Ubc-CreERt2. In these mice, Elmo1 deletion was induced by three daily intraperitoneal injections of 40 mg/kg tamoxifen dissolved in corn oil. To generate Elmo1–/–DBA mice, Elmo1–/– mice were backcrossed onto the DBA/1J background for at least 5 generations. KRN TCR transgenic mice22 (link) were a gift from Dr. Diane Mathis at the Harvard Medical School, and were bred to NOD mice to obtain the K/BxN mice23 (link), which develop progressive spontaneous arthritis. K/BxN serum was collected from 9-week old K/BxN mice by cardiac puncture. Age- and sex-matched littermate control animals were used for all experiments, and both males and females were assessed. In Elmo1fl/flLyz2-Cre mice, reduced disease severity was observed in female, but not in male mice (data not shown). All animal procedures were approved by and performed according to guidelines of the Institutional Animal Care and Use Committee (IACUC) at the University of Virginia.
Arandjelovic S., Perry J.S., Lucas C.D., Penberthy K.K., Kim T.H., Zhou M., Rosen D.A., Chuang T.Y., Bettina A.M., Shankman L.S., Cohen A.H., Gaultier A., Conrads T.P., Kim M., Elliott M.R, & Ravichandran K.S. (2019). A non-canonical role for the engulfment gene ELMO1 in neutrophils that promotes inflammatory arthritis. Nature immunology, 20(2), 141-151.
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6

Myeloid-specific Inducible K3 Knockout Mouse

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The K3hypo and K3int mice were reported previously.39 To create the myeloid‐specific inducible K3 knockout (K3KO) mouse line, K3f/f mice (exon 2 of the K3 gene is flanked by 2 loxP sites) were crossed with CX3CR1‐Cre (tamoxifen‐inducible, the Jackson Laboratory) mice. Nude mice (007850) and SCID mice (001303) were commercially available from the Jackson Laboratory. Animal experimental procedures were performed in accordance with National Institutes of Health (NIH) guidelines on animal care and all protocols were approved by the Institutional Animal Care and Use Committee of the Cleveland Clinic.
Zhevlakova I., Xiong L., Liu H., Dudiki T., Ciocea A., Podrez E, & Byzova T.V. (2022). Opposite roles of Kindlin orthologs in cell survival and proliferation. Cell Proliferation, 55(9), e13280.
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7

Conditional Knockout Mice for Immune Research

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All the animal experiments were conducted in accordance to ARRIVE guidelines and approved by the institutional animal care and use committees (IACUC) of Northwestern University (protocol #IS00015862). In addition, all the methods were performed in accordance with the relevant guidelines and regulations. C57BL/6, CD11c (Itgax)Cre (strain #8068), GfapCre (strain #24098), LysM (Lyz2)Cre (strain #4781), and Cx3cr1Cre (strain #25524) mice were purchased from the Jackson Laboratory (Bar Harbor, ME). Il27p28fl/fl mice were previously reported29 (link). All mice were bred in a specific pathogen-free facility at Northwestern University Feinberg School of Medicine.
Kim D., Kim S., Kang M.S., Yin Z, & Min B. (2023). Cell type specific IL-27p28 (IL-30) deletion in mice uncovers an unexpected regulatory function of IL-30 in autoimmune inflammation. Scientific Reports, 13, 1812.
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8

Conditional Elmo1 Knockout Mice

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C57BL/6J, DBA/1J, NOD, Mrp8-Cre, Cx3cr1-Cre, Lyz2-Cre, and Ubc-CreERt2 mice were obtained from Jackson Laboratories. Elmo1fl/fl and Elmo1–/– mice have been described previously24 (link). To generate mice with deletion of Elmo1 in myeloid cells, neutrophils or macrophages, Elmo1fl/fl mice were crossed to Lyz2-Cre, Mrp8-Cre or Cx3cr1-Cre mice, respectively. To generate mice with inducible deletion of Elmo1, Elmo1fl/fl mice were crossed to Ubc-CreERt2. In these mice, Elmo1 deletion was induced by three daily intraperitoneal injections of 40 mg/kg tamoxifen dissolved in corn oil. To generate Elmo1–/–DBA mice, Elmo1–/– mice were backcrossed onto the DBA/1J background for at least 5 generations. KRN TCR transgenic mice22 (link) were a gift from Dr. Diane Mathis at the Harvard Medical School, and were bred to NOD mice to obtain the K/BxN mice23 (link), which develop progressive spontaneous arthritis. K/BxN serum was collected from 9-week old K/BxN mice by cardiac puncture. Age- and sex-matched littermate control animals were used for all experiments, and both males and females were assessed. In Elmo1fl/flLyz2-Cre mice, reduced disease severity was observed in female, but not in male mice (data not shown). All animal procedures were approved by and performed according to guidelines of the Institutional Animal Care and Use Committee (IACUC) at the University of Virginia.
Arandjelovic S., Perry J.S., Lucas C.D., Penberthy K.K., Kim T.H., Zhou M., Rosen D.A., Chuang T.Y., Bettina A.M., Shankman L.S., Cohen A.H., Gaultier A., Conrads T.P., Kim M., Elliott M.R, & Ravichandran K.S. (2019). A non-canonical role for the engulfment gene ELMO1 in neutrophils that promotes inflammatory arthritis. Nature immunology, 20(2), 141-151.
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9

Microglial Ribosome Tagging in Mice

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Seven weeks old female C57BL/6 J mice, weighing approximately 20 g, were purchased from Daehan Bio link (DBL, Eumseong, South Korea) and maintained in a temperature (23–25 °C) and humidity controlled chamber with a 12 h light/dark cycle (light exposure during 7am to7pm). The mice had access to standard chow (DBL) and water ad libitum. CX3CR1-Cre (#025524) and RPL22-HA (#011029) mice were purchased from Jackson Laboratory. CX3CR1-Cre mice express Cre recombinase under the direction of the CX3CR1 promoter in microglia. The RPL22-HA mice carry the HA-affinity tag adjacent to the ribosomal protein Rpl22, separated from the natively expressed terminal exon by loxP recognition sites. Hence, the tagged version of Rpl22 is only expressed following Cre mediated recombination. The RPL22-HA mouse was bred into the CX3CR1-Cre mouse line to obtain RPL22-HA expressing homozygous mice. All animal care and experimental procedures were approved by the Institutional Animal Care and Use Committee (IACUC) at the Incheon National University (permission number: INU-2016-001).
Yang H.R., Tu T.H., Jeong D.Y., Yang S, & Kim J.G. (2020). Obesity induced by estrogen deficiency is associated with hypothalamic inflammation. Biochemistry and Biophysics Reports, 23, 100794.
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10

Mouse Strain Acquisition and Utilization

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C57BL/6J, Cx3cr1-cre, Villin-cre,and Alb-Cre mice were purchased from the Jackson
Laboratories. PEPCK-cre mice were provided by Volker Haase
(Vanderbilt University) 54 (link).
Morioka S., Kajioka D., Yamaoka Y., Ellison R.M., Tufan T., Werkman I.L., Tanaka S., Barron B., T Ito S., Kucenas S., Okusa M.D, & Ravichandran K.S. (2022). Chimeric efferocytic receptors improve apoptotic cell clearance and alleviate inflammation. Cell, 185(26), 4887-4903.e17.
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