Falcon hts fluoroblok 24 well inserts

The migration and invasion ability of cells were determined by the Boyden chamber technique (transwell analysis). The cell migration assay was performed with Falcon HTS FluoroBlok 24-well inserts (BD Biosciences). The 24-well Collagen-Based Cell Invasion Assay (Millipore) was used in the cell invasion assay. In brief, we rehydrated each insert by adding serum-free medium, replacing it with serum-free suspension with equal amounts of cells in the upper chamber, and incubating the cells for 12 to 24 hours to allow the cells to migrate toward/invade the lower chamber containing 10% FBS. After removing the non-invading cells in the upper chamber, the cells invading through the inserts were stained with the supplied dye, dissolved in extraction buffer, and transferred to 96-well plates for colorimetric reading at 560 nm by using ELISA microplate reader (GloMax Discover, Promega) [42 (link), 43 (link)].

Cell migration assay was performed using Falcon HTS FluoroBlok 24-well inserts (BD Biosciences, Franklin Lakes, NJ, USA) and the cell invasion assay was performed using the 24-well Collagen-based Cell Invasion Assay (Millipore, Beverly, MA, USA). Briefly, we added serum-free medium to rehydrate each insert, then replaced it with a serum-free suspension with equal numbers of cells in the upper chamber, followed by a 12- to 24-h incubation period to allow cells to migrate toward (i.e., invade) the lower chamber, which contained medium with 10% fetal bovine serum. After removal of the noninvading cells in the upper chamber, cells that invaded through the inserts were stained, lysed in extraction buffer, and transferred to 96-well plates for colorimetric readings at 560 nm.

Migration and invasion ability of cells were determined by Boyden chamber technique (transwell analysis). The cell migration assay was carried out with Falcon HTS FluoroBlok 24-well inserts (BD Biosciences). The cell invasion assay was performed using the 24-well Collagen-Based Cell Invasion Assay (Millipore). Briefly, we rehydrated each insert by adding serum-free medium, then replaced it with serum-free suspension with equal amounts of cells in the upper chamber, and incubated them for 12 to 24 hours to let cells migrate toward/invade the lower chamber containing 10% FBS. After removing the non-invading cells in the upper chamber, cells invading through the inserts were stained with provided dye, dissolved in extraction buffer, and transferred to 96-well plates for colorimetric reading at 560 nm.