To measure the colour of the developed colouring formulations, a colourimeter (model CR-400, Konica Minolta Sensing, Inc., Osaka, Japan) equipped with specific tool for granular materials (model CR-A50) was used as reported by the authors Pereira et al. [17 (link)]. The analysis of the colour parameters was made in the CIE L*a*b* colour space, through the illuminant C and the diaphragm aperture was 8 mm. The obtained data were analyzed using a “Spectra Magic Nx” (version CM-S100W 2.03.0006 software, Konica Minolta). The colour measurements were achieved after preparing the colorant preparation (t0) and every 4 weeks of storage, until it reached 12 weeks of shelf life.
Cr a50
Manufactured by Konica Minolta
Sourced in Japan
The Konica Minolta CR-A50 is a compact and versatile laser capture microdissection (LCM) system designed for precise cell sampling. It features a high-resolution camera, precise laser targeting, and a user-friendly interface.
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Lab products found in correlation
Cr 400, by Konica Minolta (8 mentions)
Spectra magic nx, by Konica Minolta (5 mentions)
Chroma meter cr 400, by Konica Minolta (2 mentions)
Spectramagic nx software, by Konica Minolta (2 mentions)
Aqualab 4te, by METER Group (1 mentions)
Cm 700d, by Konica Minolta (1 mentions)
Minolta cr 300, by Konica Minolta (1 mentions)
Aqualab vapor sorption analyzer, by METER Group (1 mentions)
Chroma meter cr 300, by Konica Minolta (1 mentions)
14 protocols using cr a50
1
Colorimetric Analysis of Colouring Formulations
2
Grape Pomace and Jelly Candy Color Analysis
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The color of the grape pomace and jelly candy samples was analyzed in conformity with the method reported by Spinei and Oroian [20 (link)], using the granular material attachment CR-A50 (Konica Minolta, Inc., Tokyo, Japan). A glass Petri dish containing grape pomace and jelly candies was placed above the attachment.
3
Colorant Potential Evaluation of Extracts
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The evaluation of the colorant potential of the extract was carried out by measuring the colour and the measurement of the colouring compounds by chromatography, in order to corroborate the data provided by the MRS. The colour was measured using a colorimeter (model CR-400, Konica Minolta Sensing, Inc., Osaka, Japan) with an adapter for granular materials (model CR-A50), according to a procedure described by Pereira et al. [24 (link)]. The measurements were made in the CIE L*a*b* colour space, using the illuminant C and a diaphragm aperture of 8 mm. Data were processed with the “Spectra Magic Nx” (version CM-S100W 2.03.0006 software, Konica Minolta). Quantitation of anthocyanin compounds was accomplished by chromatography using an HPLC-DAD-ESI/MS system as described in Section 3.4 .
4
Proximate Composition and Color Analysis
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Moisture content was determined at 105 °C by means of an automatic moisture analyzer (Radwag Wagi Elektroniczne, Radom, Poland). Water activity (aw) was determined by using the water activity meter Aqua Lab 4TE (Meter Group Inc., Pullman, WA, USA) according to the manufacturers’ instructions. Protein content (N × 5.7) was analyzed according to the AACC approved method 46-11.02 (AACC, 2000 ), whereas fat was extracted and determined by Soxhlet apparatus using diethyl ether as solvent, and total dietary fiber was determined by the enzymatic-gravimetric procedure (AOAC, 1995 ). Carbohydrates were calculated by difference.
Colorimetric evaluations of red index (a*), yellow index (b*) and brown index (BI, defined as 100-L*) were carried out under D65 illuminant by using a spectro-colorimeter CM-700d (Konica Minolta Sensing, Osaka, Japan) equipped with a pulsed xenon lamp and granular materials attachment CR-A50 (Konica Minolta Sensing, Osaka, Japan).
Chemical determinations were carried out in triplicate, whereas colorimetric evaluations were made with five replications.
Colorimetric evaluations of red index (a*), yellow index (b*) and brown index (BI, defined as 100-L*) were carried out under D65 illuminant by using a spectro-colorimeter CM-700d (Konica Minolta Sensing, Osaka, Japan) equipped with a pulsed xenon lamp and granular materials attachment CR-A50 (Konica Minolta Sensing, Osaka, Japan).
Chemical determinations were carried out in triplicate, whereas colorimetric evaluations were made with five replications.
5
Color Measurement of Baked Breadsticks
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Color measurements were carried out on the day of baking by using a tristimulus colorimeter (Minolta CR-300, Konica Minolta Sensing, Osaka, Japan) equipped with a measuring head CR-300 and previously calibrated against a white tile. To avoid inaccurate measurements due to the limited width of the samples, 60 breadsticks per batch were finely ground and placed into the granular material attachment (CR-A50, Konica Minolta Sensing, Osaka, Japan) of the colorimeter. The results were expressed in accordance with the Hunter Lab color space and the parameters acquired were lightness L*, redness (a*), and yellowness (b*). Total color difference (∆E) was also calculated by using the following equation:
For each sample, a total of ten repetitions were made.
For each sample, a total of ten repetitions were made.
6
Color Analysis of Emulsions
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The colour of the emulsions was assessed using a Chroma Meter CR-400 (Konica Minolta, Inc., Tokyo, Japan). The results were express in accordance with the CIELAB system with reference to illuminate D65 and a visual angle of 10°. The parameters determined were L* (L* = 0 (black) and L* = 100 (white)), a* (−a* = greenness and +a* = redness), b* (−b* = blueness and +b* = yellowness). The samples were poured into a granular-materials attachment CR-A50 (Konica Minolta, Inc., Tokyo, Japan) and measured in triplicate.
7
Colorimeter Analysis of Granular Materials
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The color measurement was performed to assess possible differences after incorporation. A colorimeter (model CR-400; Konica Minolta Sensing, Inc., Tokyo, Japan) coupled to an adapter for granular materials (model CRA50) was used, following the methodology previously described by Roriz et al. [18 (link)].
The values of the three-dimensional coordinates CIE L* a* b* were obtained in a computerized system with a type C illuminant and an 8 mm diameter diaphragm, and for data processing, the Spectra Magic Nx software (CM-S100W 2.03.0006 version, Konica Minolta, Japan) was used. Regarding the three-dimensional coordinates obtained, L* represents luminosity, a* represents chromaticity on an axis from green (−) to red (+), and b* represents chromaticity on an axis from blue (−) to yellow (+).
The values of the three-dimensional coordinates CIE L* a* b* were obtained in a computerized system with a type C illuminant and an 8 mm diameter diaphragm, and for data processing, the Spectra Magic Nx software (CM-S100W 2.03.0006 version, Konica Minolta, Japan) was used. Regarding the three-dimensional coordinates obtained, L* represents luminosity, a* represents chromaticity on an axis from green (−) to red (+), and b* represents chromaticity on an axis from blue (−) to yellow (+).
8
Colorimetric Analysis of Powdered Samples
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The powdered samples were placed on a adapter for granular materials (model CR-A50, Konica Minolta Sensing, Inc., Sakai, Osaka, Japan) to reduce external interferences and data were collected in three different points on each set of samples with a colorimeter (model CR-400, Konica) previously calibrated using the standard white plate. Using illuminant C and the diaphragm opening of 8 mm, the CIE L*a*b* color space values were registered through the computerized system using the SpectraMagic Nx (version CM-S100W) color data software. Average values were considered to determine the color coordinates, where L* represents lightness, a* represents chromaticity on a green (–) to red (+) axis, and b* represents chromaticity on a blue (–) to yellow (+) axis. The total color difference (ΔE*) was calculated according to the CIEDE2000 equation [44 ].
9
Anthocyanin Concentration and Color Analysis
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To evaluate the concentration of anthocyanins in the different formulations, stored at distinct temperatures, these were dissolved in water to a final concentration of 5 mg/mL and were analysed as described in Section 3.2.4 .
In what concerns the colour parameters, a colourimeter (model CR-400, Konica Minolta Sensing, Inc., Osaka, Japan) equipped with a specific tool for granular materials (model CR-A50) was used to analyse the powders, as reported by the authors Pereira et al. [39 (link)]. For that purpose, the colour parameters were obtained in the CIE L*a*b* colour space, through the illuminant C with a diaphragm aperture of 8 mm, and the Spectra Magic Nx software (version CM-S100W 2.03.0006, Konica Minolta) was used to analyse the obtained data.
In what concerns the colour parameters, a colourimeter (model CR-400, Konica Minolta Sensing, Inc., Osaka, Japan) equipped with a specific tool for granular materials (model CR-A50) was used to analyse the powders, as reported by the authors Pereira et al. [39 (link)]. For that purpose, the colour parameters were obtained in the CIE L*a*b* colour space, through the illuminant C with a diaphragm aperture of 8 mm, and the Spectra Magic Nx software (version CM-S100W 2.03.0006, Konica Minolta) was used to analyse the obtained data.
10
Colorimetric Analysis of Granular Samples
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A colorimeter (model CR-400, from Konica Minolta Sensing, Inc., Japan), with an adapter for granular materials (model CR-A50) was used to measure the color of the samples. Using the illuminant C and diaphragm aperture of 8 mm, the CIE L*a*b* color space values were registered using a data software "Spectra Magic Nx" (version CM-S100W 2.03.0006), from Konica Minolta company (Japan). Before starting the measurements the instrument was calibrated against a standard white tile (Pereira et al., 2015) . The colour of three samples from each batch was measured in three different points, for each dose and at each time point, being considered the average value.
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