The electron microscopy was conducted at the Shanghai Jiao Tong University Instrumental Analysis Center. Briefly, the liver tissues from Cp-LKO and littermate controls were excised into pieces of 1 mm3 and fixed overnight in 2.5% glutaraldehyde at room temperature. The samples were post-fixed in potassium ferrocyanide-reduced osmium tetroxide (Electron Microscopy Sciences, USA) for 1.5 h and dehydrated in a bath with gradually increasing concentrations of ethanol (50%, 70%, 90%). The ethanol was then eliminated with gradually increasing acetone concentrations (90% ethanol: 90% acetone = 1:1, 90% acetone, 100% acetone). Further, the tissues were incubated with a mixture of acetone: EPON (liquid epoxy resin) (1:1,1:2, and 1:3) and ultimately embedded with pure EPON (Electron Microscopy Sciences, USA). The picture was captured using a biological transmission electron microscope (Tecnai G2 Spirit BioTWIN, Thermo Fisher Scientific, USA). The liver tissues from the BCS-treated Cp-LKO mice and the control group were analyzed following the same procedures.
Pure epon
Manufactured by Electron Microscopy Sciences
Sourced in United States
Pure EPON is an epoxy resin formulation used in electron microscopy sample preparation. It serves as a base material for embedding and embedding samples for ultrathin sectioning and examination under an electron microscope.
Automatically generated - may contain errors
2 protocols using pure epon
1
Ultrastructural Analysis of Liver Tissues in Cp-LKO Mice
2
Ultrastructural Analysis of Liver Tissues in Cp-LKO Mice
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The electron microscopy was conducted at the Shanghai Jiao Tong University Instrumental Analysis Center. Briefly, the liver tissues from Cp-LKO and littermate controls were excised into pieces of 1 mm3 and fixed overnight in 2.5% glutaraldehyde at room temperature. The samples were post-fixed in potassium ferrocyanide-reduced osmium tetroxide (Electron Microscopy Sciences, USA) for 1.5 h and dehydrated in a bath with gradually increasing concentrations of ethanol (50%, 70%, 90%). The ethanol was then eliminated with gradually increasing acetone concentrations (90% ethanol: 90% acetone = 1:1, 90% acetone, 100% acetone). Further, the tissues were incubated with a mixture of acetone: EPON (liquid epoxy resin) (1:1,1:2, and 1:3) and ultimately embedded with pure EPON (Electron Microscopy Sciences, USA). The picture was captured using a biological transmission electron microscope (Tecnai G2 Spirit BioTWIN, Thermo Fisher Scientific, USA). The liver tissues from the BCS-treated Cp-LKO mice and the control group were analyzed following the same procedures.
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