Ab5168

Antibodies against anti-H3 (1:5000; ab1791), anti-H4 (1:5000; ab10158) and H3pT11 (1:5000; ab5168) were purchased from Abcam; antibodies against H3K79me1 (1: 1000; A2367), H3K79me2 (1: 5000; A2368), H3K79me3 (1:5000; A2369), anti-His (1:5000; AE0039) and anti-FLAG (1:5000; AE024) were purchased from Abclonal; antibody against Sir2 (1:500; sc-6667) was purchased from Santa Cruz Biotechnology; antibodies against GAPDH (1:10,000; 10494-1-AP), GFP (1:5000; 66002-1-1 g), Myc (1:5000; 60003-2-1 g), goat polyclonal anti-mouse IgG (1:5000; SA00001-1) and goat polyclonal anti-rabbit IgG (1:5000; SA00001-2) were obtained from Proteintech; antibody against FLAG M2 (1:3000; F1804-1MG) was obtained from Sigma-Aldrich; antibody against H4K16ac (1:2000; 07-329) was obtained from EMD Millipore; antibody against CBP (1:2000; Abs130593) was purchased from Absin Bioscience Inc. The custom-made antibodies against Acs2, Pyk1, Sam1 and Shm2 were produced by Covance Inc. and their specificity was verified by immunoblots of cell lysates of the corresponding histone point mutants, gene deletion or knockdown mutants^{31 (link)}.

Antibodies against H3 (1: 5000; ab1791), and H3pT11 (1:3000; ab5168) were purchased from Abcam; antibody against FLAG M2 (1:3000; F1804-1MG) was obtained from Sigma-Aldrich; antibodies against Myc (1:5000; 60003-2-1 g), PHGDH (1:3000; 14719-1-AP), GAPDH (1:10000; 10494-1-AP), PSAT1 (1:3000; 10501-1-AP), PSPH (1:3000; 14513-1-AP), PKM1 (1:2000; 15821-1-AP), p21 (1:3000; 10355-1-AP), PCAF (1:3000; 13983-1-AP), SIRT1 (1:2000; 13161-1-AP), 6×His (1:5000; HRP-66005), GST (1:5000; HRP-66001), beta-actin (1:10000; 20536-1-AP), and goat polyclonal anti-mouse IgG (1:5000; SA00001-1) were obtained from proteintech; antibodies against PKM2 (1:3000; 4053 S), p300 (1:5000; 54062), and ATF4 (1:1000; 11815) were purchased from Cell Signaling Technology; antibodies against mouse CDKN1A/p21 (1:3000; A11454), SOD1 (1:2000; A0274), SOD2 (1:1000; A19576), Caspase 3 (1:2000; A11040), PPARγ (1:1000; A11183), PGC1α (1:1000; A220995), and mouse PKM2 (1:5000; A19102) were purchased from Abclonal; antibody against SIRT6 (1:2000; 200499-6C9) was purchased from ZENBO; antibody against PKM2 K433ac (1:500) was custom-made in Abclonal. Antibody against PKM2K305ac was a gift from Dr. Qunying Lei (Fudan University). The specificity of the custom-made antibodies was confirmed by dot blots with peptides or immunoblots with cell extract of corresponding mutants.

Antibodies against H3 (1: 5000; ab1791), H3pT11 (1:3000; ab5168) and SETX (1:3000; ab243904) were purchased from Abcam; antibody against FLAG M2 (1:3000; F1804-1MG) was obtained from Sigma-Aldrich; antibodies against Sir2 (1:500; sc-6667) and LC3 (1:1000; sc-398822) were purchased from Santa Cruz Biotechnology; antibodies against Myc (1:5000; 60003-2-1 g), GAPDH (1:10000; 10494-1-AP), beta-actin (1:10000; 20536-1-AP), GFP (1:5000; 66002-1-Ig) and goat polyclonal anti-mouse IgG (1:5000; SA00001-1) were obtained from Proteintech; antibody against Rif1 (1:3000; 95558 S) was purchased from Cell Signaling Technology; antibodies against CDKN1A/p21 (1:3000; A11454), and PPP1CA (1:5000; A12468) were purchased from Abclonal; antibody against CBP (1:3000; abs130593) was purchased from Absin. Antibody against Glc7 (1:5000) was custom-made in Abclonal and its specificity was confirmed by dot blots with Glc7 peptide (Supplementary Fig. S11b). H3pT11 was validated for ChIP assay in H3T11A mutant and PKM2 knockdown HUVECs (Supplementary Fig. S11c, d).