Raw white eggshells were provided by American Dehydrated Foods, Atlanta, GA. Ethanol (99.5% purity, absolute), nitric acid (65% HNO3), ammonium hydroxide (28% NH4OH), semiconductor grade phosphoric acid (85% H3PO4), poly(lactic acid) (PLA, Mw: 76000), anhydrous chloroform (≥99% purity), and dimethyl sulfoxide (≥99% DMSO) were purchased from Sigma-Aldrich Chemical Company St. Louis, MO. Human osteoblast cells (ATCC CRL 11372) were purchased from American Type Culture Collection (ATCC); Dulbecco's modified eagle medium (DMEM) was purchased from Fisher scientific. Penicillin and streptomycin were purchased from Lonza. Fetal bovine serum (FBS Lot no. 8SB013) was purchased from VWR. Hematoxylin and eosin reagents were purchased from Sigma-Aldrich.
Hematoxylin and eosin reagents
Manufactured by Merck Group
Hematoxylin and eosin (H&E) reagents are a set of stains used in histology and pathology laboratories for the staining and visualization of cellular structures. Hematoxylin stains nuclei blue, while eosin stains cytoplasm and other structures pink. This staining technique provides contrast and allows for the identification of different cell types and tissue structures under a microscope.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Ethanol, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Ammonium hydroxide, by Merck Group (1 mentions)
Nitric acid, by Merck Group (1 mentions)
Penicillin, by Lonza (1 mentions)
Streptomycin, by Lonza (1 mentions)
2 protocols using hematoxylin and eosin reagents
1
Eggshell-Derived Biomaterials for Osteoblast Culture
2
Hematoxylin and Eosin Staining of Paraffin Sections
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Sections of 3 μm were prepared from paraffin-embedded tissue blocks and stained using commercial hematoxylin and eosin reagents (Sigma-Aldrich, NJ). Briefly, slides were deparaffinized and processed as follows: incubation at 75°C in an oven for 10 minutes; three 5-minute rinses in xylene; three 5-minute rinses in 100% ethanol; one 5-minute rinse in each of the 95% ethanol, 75% ethanol, and 50% ethanol solutions, consecutively; one 3-minute rinse in running tap water; one 5-minute staining incubation immersed in Mayer's hematoxylin solution. Slides were then rinsed once in running cold tap water for 5 minutes, 10 times in 95% acid ethanol for 3 second each and stained in eosin Y solution for 30 seconds. Slides were dehydrated by one 3minute immersion in each of the 50% ethanol, 75% ethanol, and 95% ethanol solutions, and three 3-minute immersions in 100% ethanol, and cleared in three 3-minute immersions in xylene. Data from 5 representative nonoverlapping fields selected under 400× magnification were pooled for each clinical specimen.
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