Pgl4.17 luc

Dried crude herbs (Chaihu-Shugan-San, CSS) were purchased and identified in the pharmacy of Xiangya Hospital (Changsha, China). Voucher specimens (CH-NO-15021714, BS-NO-15021411, CP-NO-15020914, XF-NO-15021118, CX-NO-15022413, ZK-NO-15020706, GC-NO-15022715) were obtained and kept in the Laboratory of the Institute of Integrative Medicine, Central South University. Glycyrrhetinic acid (GA) was purchased from the Beijing Hengyuan Qitian Chemical Industry (Beijing, China). Rifampicin (RIF), pregnenolone 16α-carbonitrile (PCN), dimethyl sulfoxide (DMSO), and phenobarbital (PB) were obtained from Sigma-Aldrich (St. Louis, MO, USA). The reverse transcription system, dual-luciferase reporter assay system, pGL4.17-Luc, and pGEM-T constructs were supplied by Promega (Madison, WI, USA).

Rifampicin (RIF), pregnenolone 16α-carbonitrile(PCN), phenobarbital (PB), dimethyl sulfoxide (DMSO), diethyl pyrocarbonate (DEPC) were purchased from Sigma-Aldrich (St. Louis, MO, USA). RIF, PCN, and PB were dissolved in DMSO at concentrations appropriate for the specific studies in which they were used. Trypsin and Taq DNA polymerase were purchased from Gibco BRL (Grand Island, NY, USA). Reverse transcription system, dual-luciferase reporter assay system, pGL4.17-Luc and pGEM-T constructs were purchased from Promega (Madison, WI, USA).