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3 protocols using n butyl alcohol

1

Comprehensive Biochemical Analysis Techniques

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Anthrone reagent (Thermo Scientific, Waltham, MA, USA), sulfuric acid (HiMedia, Mumbai, India), Congo red (HiMedia), n-butyl alcohol (HiMedia), methanol (HiMedia), hematoxylin (Mayer’s reagent) (HiMedia), acetic anhydride (Sigma-Aldrich, St. Louis, MO, USA), chloroform (HiMedia), ammonium buffer solution (HiMedia), hydrochloric acid (HiMedia), lead(II) acetate trihydrate (HiMedia), ninhydrin (HiMedia), Fehling A and B solutions (HiMedia), potassium ferricyanide (HiMedia), trichloroacetic acid (HiMedia), DPPH (2,2-diphenyl-1-picrylhydrazyl) (Sigma-Aldrich), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) (Invitrogen, Carlsbad, CA, USA), (H2DCFDA) (dichlorofluorescein diacetate) (Invitrogen), LysoTracker Red (Invitrogen), and MitoTracker Red CMX-ROS (Invitrogen).
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2

Antioxidant and Cytotoxicity Assays

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Bovine serum
albumin (BSA), 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH), 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic
acid) (ABTS), nicotinamide adenine dinucleotide (NADH), nitroblue
tetrazolium (NBT), disodium hydrogen phosphate, n-butyl alcohol, 2-thiobarbituric acid (TBA), phenazine methosulfate
(PMS), glutathione reduced, potassium persulfate, gallic acid, quercetin
dihydrate, ferric chloride, sodium nitrite, methanol, and cumene hydroperoxide
were obtained from HiMedia Laboratories Pvt., Ltd. (Mumbai, India).
Glacial acetic acid, aluminum chloride, silver nitrate, hydrogen peroxide
(H2O2), and 5,5′-dithio-2-nitrobenzoic
acid (DTNB) were obtained from Merck Specialities Pvt., Ltd. (Mumbai,
India). Potassium ferricyanide and ferrous chlorides were obtained
from Loba Chemie Pvt., Ltd. (Mumbai, India). 1-Chloro-2,4-dinitrobenzene
(CDNB), cupric sulfate, trichloroacetic acid (TCA), Folin–Ciocalteu’s
reagent, sodium hydroxide, and ascorbic acid were obtained from SD
Finechem Ltd. (Mumbai, India). Doxorubicin (Getwell Oncology Pvt.,
Ltd., Haryana, India) was purchased from a local pharmacy.
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3

Cellular Assays for Cytotoxicity and Oxidative Stress

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Anthrone reagent (Thermo Scientific), Sulfuricacid (Hi-Media), n-Butyl alcohol (HiMedia), Methanol (HiMedia), DPPH (2,2-Diphenyl-1-picrylhydrazyl) (Sigma-Aldrich), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) (Invitrogen), (H 2 DCFDA) (dichlorofluoresceindiacetate) (Invitrogen), LysoTracker RedDND99 (Invitrogen), JC-1 (Invitrogen), Hoechst (Invitrogen), FBS, Antibiotic-antimycotic solution, Dulbecco's Modified Eagle Medium (DMEM) (Gibco), Trypsin (HiMedia).
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