Measurement of MMP-9, VEGF, and IGF1 protein content was conducted using a Human IGF1 SimpleStep ELISA® Kit (Abcam, Cambridge, UK), Human VEGF SimpleStep ELISA® Kit (Abcam, Cambridge, UK), and Human MMP-9 ELISA Kit (Invitrogen, Waltham, MA, USA), respectively. All procedures were conducted in accordance with the manufacturer’s protocol. The endpoint reading (color intensity) of all analytes was measured at 450 nm and protein concentration was calculated from the standard curve.
Human igf1 simplestep elisa kit
Manufactured by Abcam
Sourced in United Kingdom
The Human IGF1 SimpleStep ELISA® Kit is a solid-phase enzyme-linked immunosorbent assay designed to measure the concentration of IGF1 in human serum, plasma, and cell culture supernatants.
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Lab products found in correlation
Human igfbp 3 simplestep elisa kit, by Abcam (2 mentions)
Decorin human elisa kit, by Abcam (2 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Human vegf simplestep elisa kit, by Abcam (1 mentions)
Human mmp 9 elisa kit, by Thermo Fisher Scientific (1 mentions)
Ab211651, by Abcam (1 mentions)
Ab211652, by Abcam (1 mentions)
4 protocols using human igf1 simplestep elisa kit
1
Quantification of MMP-9, VEGF, and IGF1
2
Fasting Serum Biomarker Analysis
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Resting fasting venous blood samples (at least 10 h of overnight fasting) were collected before any physical tests on the day of baseline and postintervention body composition assessments using serum separating tubes. After blood collection, the blood was left at room temperature for 30 min and then immediately centrifuged at 1600g for 10 min. The centrifuged blood samples were transported to the Exercise Medicine Research Institute (Joondalup, WA, Australia), and the supernatant was carefully aliquoted. The serum samples were then stored in a −80°C freezer at the Exercise Medicine Research Institute until further analysis. Serum levels of SPARC, OSM, IL-6, IL-15, and irisin were analyzed using a multiplexed magnetic bead–based immunoassay (MILLPLEX human myokine magnetic bead panel; Millipore, Burlington, MA), and serum levels of decorin, insulin-like growth factor-1 (IGF-1), and insulin-like growth factor binding protein-3 (IGFBP-3) were analyzed using an enzyme-linked immunosorbent assay (Human decorin ELISA kit, Human IGF1 SimpleStep ELISA kit, Human IGFBP-3 SimpleStep ELISA kit; Abcam, Cambridge, United Kingdom).
3
Salivary Biomarkers Measurement Protocol
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Saliva samples were collected from all participants that were willing and able to offer saliva through passive drool before and after the single-bout intervention. The samples were placed on ice at −80 °C and then analyzed in triplicate for concentrations of cortisol (Cohen Biochemistry lab protocol), alpha-amylase (alpha-amylase saliva ELISA, BioVendor, Brno, Czech Republic), and IGF-1 (Human IGF1 SimpleStep ELISA Kit, Abcam, Cambridge, UK). Protein concentrations in the saliva samples were measured using the bicinchoninic (BCA) assay (Pierce™ BCA Protein Assay Kit, Thermo Fisher, Waltham, MA, USA). Measurements of the salivary biomarkers were normalized to the total protein concentrations.
4
Serum Myokine and Growth Factor Profiling
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Resting blood samples were collected early in the morning for fasting specimens and at least 48 h post any exercise. The collected blood samples were processed to serum and stored at −80 °C until serum myokine analysis. Serum myokine levels for irisin, IL-6, IL-15, SPARC, OSM, and myostatin, were analysed using multiplex magnetic bead panels (HMYOMAG-56k-15 Huma, Millipore, Billerica, MA, USA), and serum decorin (ab99998, Decorin Human ELISA Kit, Abcam, Cambridge, United Kingdom), IGF-1 (ab211651, Human IGF-1 SimpleStep ELISA Kit, Abcam, Cambridge, United Kingdom), and IGFBP-3 (ab211652, Human IGFBP3 SimpleStep ELISA Kit, Abcam, Cambridge, United Kingdom) levels were analysed using appropriate enzyme-linked immunosorbent assay (ELISA) kits.
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