Glucose oxidase enzymatic method

Lipids (HDL cholesterol and triglycerides) and glucose were obtained from a total of 12 mL blood samples collected at the beginning of the BWRP. Serum glucose level was determined by the glucose oxidase enzymatic method (Roche Diagnostics, Monza, Italy) with a sensitivity of 2 mg/dL, whilst serum HDL cholesterol and triglycerides levels were measured by colorimetric enzymatic assays (Roche Diagnostics, Monza, Italy) with sensitivities of 3.09 mg/dL and 8.85 mg/dL, respectively.

Blood samples (about 10 mL) were collected at around 8:00 AM after an overnight fast. Aspartate aminotransferase (AST), T-C, HDL-C, LDL-C, TG, total bilirubin, uric acid, glucose, insulin and CRP were measured. Colorimetric enzymatic-assays (Roche Diagnostics, Monza, Italy) were used to determine serum AST, T-C, LDL-C, HDL-C, TG, uric acid and total bilirubin levels. The sensitivities of the method for each parameter were 5 U/L, 3.86 mg/dL, 3.87 mg/dL, 3.09 mg/dL, 8.85 mg/dL, 0.2 mg/dL and 0.146 mg/dL, respectively.
The serum glucose level was measured by the glucose oxidase enzymatic method (Roche Diagnostics, Monza, Italy). The sensitivity of the method was 2 mg/dL. The serum insulin concentration was determined by a chemiluminescent immunometric assay, using a commercial kit (Elecsys Insulin, Roche Diagnostics, Monza, Italy). The sensitivity of the method was 0.2 µIU/mL.
Insulin resistance was estimated using the HOMA-IR method [39 (link)]. CRP was measured using an immunoturbidimetric assay (CRP RX, Roche Diagnostics GmbH, Mannheim, Germany). The sensitivity of the method was 0.03 mg/dL. APRI was calculated by means of the following formula: (AST [IU/L]/40)/platelet count [10⁹/L] * 100.

Blood samples (a total of about 20 mL) were collected at around 8:00 AM after an overnight fast only at the beginning of the BWRP. Lipids (high-density lipoprotein (HDL) cholesterol and triglycerides) and glucose were measured.
Serum glucose level was measured by the glucose oxidase enzymatic method (Roche Diagnostics, Monza, Italy). The sensitivity of the method was 2 mg/dL [1 mg/dL = 0.06 mmol/L].
Colorimetric enzymatic-assays (Roche Diagnostics, Monza, Italy) were used to determine serum HDL cholesterol and triglycerides levels. The sensitivities of the assays were 3.09 mg/dL [1 mg/dL = 0.03 mmol/L] and 8.85 mg/dL [1 mg/dL = 0.01 mmol/L], respectively.