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The HmlΔ::Gal4 is a genetic construct that drives the expression of the yeast transcriptional activator Gal4 in the hemocytes of Drosophila melanogaster. It is a tool used to study the function and development of blood cells in the fruit fly.

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Lab products found in correlation

W1118, by Bloomington Drosophila Stock Center (2 mentions) Lsp2 gal4, by Bloomington Drosophila Stock Center (2 mentions) Elav gal4, by Bloomington Drosophila Stock Center (2 mentions) Uas 5ht1brnai, by Bloomington Drosophila Stock Center (1 mentions) Uas 5ht2brnai, by Bloomington Drosophila Stock Center (1 mentions) He gal4, by Bloomington Drosophila Stock Center (1 mentions) Lzgal4, by Bloomington Drosophila Stock Center (1 mentions) Uas gfp, by Bloomington Drosophila Stock Center (1 mentions)

4 protocols using hmlδ gal4

1

Drosophila Genetics and Fly Food Preparation

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Flies were cultured on yeast/molasses-based standard fly food (recipe: 10L H2O, 138g agar, 220g molasses, 750g malt extract, 180 dry yeast, 800g corn flour, 100g soy flour, 62.5ml propionic acid, 20g Methyl 4-Hydroxybenzoate, and 72ml ethanol). For GeneSwitch experiments, 86 mg RU486 was additionally dissolved in the ethanol for 200 μM final concentration. Flies were maintained at 25°C with a 12h light/dark cycle and 65% humidity. All flies used in the experiments reported were females.
Fly lines HmlΔ::Gal4, LSP2::Gal4, Elav::Gal4 and W1118 were provided by the Bloomington Drosophila Stock Center. RNAi line MANFRNAiv12834 (UAS::MANFRNAi) was obtained from the Vienna Drosophila RNAi Center. These construct has no predicted off-targets. MANFRNAiv12834 was combined with a transgene driving Dicer overexpression (UAS::DICER2) to achieve efficient MANF knock-down19 (link).
We received the following lines as gifts: UAS::MANF, (T. Heino); NP1::Gal4 (D. Ferrandon); PPL::Gal4 (M. Pankratz); Upd3::Gal4 (N. Perrimon); C7::Gal4 (M. Uhlivora); UAS::Upd2 (M. Zeidler); UAS::Upd3 (N. Buchon); 2xStat::GFP (E. Bach).
Sousa-Victor P., Neves J., Cedron-Craft W., Ventura P.B., Liao C.Y., Riley R.R., Soifer I., van Bruggen N., Kolumam G.A., Villeda S.A., Lamba D.A, & Jasper H. (2019). MANF regulates metabolic and immune homeostasis in ageing and protects against liver damage. Nature metabolism, 1(2), 276-290.
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2

Rearing and Genetic Manipulation of Cabbage White Butterfly

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P. rapae larvae were collected primarily from cabbage fields in the experimental farmland of Zhejiang University, Hangzhou, China and the laboratory colony of P. rapae was reared at 25 ± 1°C and 70% relative humidity under a photoperiod of 14 hr light: 10 hr dark in a greenhouse as previously described by Zhang et al. (2005) (link).
The following fly stocks were from the Bloomington Drosophila Stock Center: HmlΔ-Gal4 (30139); UAS-5-HT1B RNAi (27634, 25833); UAS-5-HT2B RNAi (25874, 60488). The 5-HT1B control and 5-HT1BΔIII-Vflies were generous gifts from Leslie Vosshall (Gasque et al., 2013 (link)).
Qi Y.X., Huang J., Li M.Q., Wu Y.S., Xia R.Y, & Ye G.Y. (2016). Serotonin modulates insect hemocyte phagocytosis via two different serotonin receptors. eLife, 5, e12241.
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3

Drosophila Strains for Genetic Studies

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These Drosophila strains were obtained from the Bloomington Stock Center: He-GAL4 (RRID:BDSC_8699); HmlΔ-GAL4 (RRID:BDSC_30141); Lz-GAL4; UAS-GFP (RRID:BDSC_6313); UAS-GFP (RRID:BDSC_6874); UAS-TRE-Red (RRID:BDSC_59012). Pxn-GAL4 (FBtp0022490) was kindly donated by Utpal Banerjee. All “wild-type” strains were relevant GAL4 driver outcrossed to either UAS-GFP or to w; P(w+).
Myers A.L., Harris C.M., Choe K.M, & Brennan C.A. (2018). Inflammatory Production of Reactive Oxygen Species by Drosophila Hemocytes Activates Cellular Immune Defenses. Biochemical and biophysical research communications, 505(3), 726-732.
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4

Drosophila Genetics and Fly Food Preparation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Flies were cultured on yeast/molasses-based standard fly food (recipe: 10L H2O, 138g agar, 220g molasses, 750g malt extract, 180 dry yeast, 800g corn flour, 100g soy flour, 62.5ml propionic acid, 20g Methyl 4-Hydroxybenzoate, and 72ml ethanol). For GeneSwitch experiments, 86 mg RU486 was additionally dissolved in the ethanol for 200 μM final concentration. Flies were maintained at 25°C with a 12h light/dark cycle and 65% humidity. All flies used in the experiments reported were females.
Fly lines HmlΔ::Gal4, LSP2::Gal4, Elav::Gal4 and W1118 were provided by the Bloomington Drosophila Stock Center. RNAi line MANFRNAiv12834 (UAS::MANFRNAi) was obtained from the Vienna Drosophila RNAi Center. These construct has no predicted off-targets. MANFRNAiv12834 was combined with a transgene driving Dicer overexpression (UAS::DICER2) to achieve efficient MANF knock-down19 (link).
We received the following lines as gifts: UAS::MANF, (T. Heino); NP1::Gal4 (D. Ferrandon); PPL::Gal4 (M. Pankratz); Upd3::Gal4 (N. Perrimon); C7::Gal4 (M. Uhlivora); UAS::Upd2 (M. Zeidler); UAS::Upd3 (N. Buchon); 2xStat::GFP (E. Bach).
Sousa-Victor P., Neves J., Cedron-Craft W., Ventura P.B., Liao C.Y., Riley R.R., Soifer I., van Bruggen N., Kolumam G.A., Villeda S.A., Lamba D.A, & Jasper H. (2019). MANF regulates metabolic and immune homeostasis in ageing and protects against liver damage. Nature metabolism, 1(2), 276-290.
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