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Allprep extraction kit

Manufactured by Qiagen
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The AllPrep extraction kit is a laboratory equipment product designed for the simultaneous purification of DNA, RNA, and proteins from a single biological sample. It facilitates the efficient extraction and separation of these molecular components, allowing for comprehensive analysis and downstream applications.

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Lab products found in correlation

Rnalater, by Qiagen (2 mentions) Hiseq 2000 system, by Illumina (2 mentions) 7500 fast real time pcr detection system, by Thermo Fisher Scientific (1 mentions) Absolute blue qpcr sybr green low rox mix, by Thermo Fisher Scientific (1 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions) Viia 7 real time pcr system, by Thermo Fisher Scientific (1 mentions) Nanodrop 2000, by Thermo Fisher Scientific (1 mentions) Sybr select, by Thermo Fisher Scientific (1 mentions) Bstui, by New England Biolabs (1 mentions) Hpaii, by New England Biolabs (1 mentions) Rneasy rna extraction kit, by Qiagen (1 mentions) Qiashredder spin column, by Qiagen (1 mentions) Nextseq 500, by Illumina (1 mentions) Truseq stranded mrna library preparation kit, by Illumina (1 mentions)

Close spelling variants of this product

AllPrep kit (307 citations) AllPrep DNA/RNA extraction kit (40 citations) AllPrep DNA/RNA FFPE extraction kit (11 citations) AllPrep DNA extraction kit (4 citations) AllPrep DNA/RNA/protein extraction kit (3 citations) AllPrep DNA/RNA Extraction Mini kit (3 citations) Allprep DNA/RNA column-based extraction kits (2 citations) AllPrep 96 DNA/RNA extraction kit (2 citations) AllPrep DNA/RNA/miRNA extraction kit (2 citations) Allprep DNA/RNA/miRNA universal extraction kit (2 citations)

12 protocols using allprep extraction kit

1

RNA Extraction and RT-qPCR Analysis

Cited 2 times
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AllPrep extraction kit (Qiagen, Hilden, Germany) was used to extract RNA. cDNA was obtained by reverse transcription using the high capacity cDNA reverse transcription kit (Applied Biosystems). ABsolute Blue qPCR SYBR Green Low ROX Mix (Thermo Scientific), 2.5 pmol of each primer and 5 ng of cDNA per reaction was used and the reaction was run on a 7500 FAST Real time PCR Detection System (Applied Biosystems) cycler with the following settings: 40x (95°C for 30 sec, 60°C for 30 sec and 72°C for 30 sec). Expression levels of the gene of interest were normalized against multiple reference genes [39 (link)] using the qbase+ software [40 (link)], according to the MIQE guidelines. The used reference genes were: EFF1A1, B-Actin, 28S, YWHAZ. Primer pairs used for RT-qPCR are shown in S1C Table.
Qureshi-Baig K., Ullmann P., Rodriguez F., Frasquilho S., Nazarov P.V., Haan S, & Letellier E. (2016). What Do We Learn from Spheroid Culture Systems? Insights from Tumorspheres Derived from Primary Colon Cancer Tissue. PLoS ONE, 11(1), e0146052.
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2

DNA and RNA Methylation Analysis

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DNA and RNA were extracted simultaneously from flash-frozen left ventricular tissue specimens using the AllPrep extraction kit (Qiagen, Germantown, MD). Nucleic acid quality and concentration was assessed by spectrophotometry (Nanodrop 2000, ThermoFisher Scientific, Waltham, MA). Analysis of the DNA methylation was performed from 250 ng of genomic DNA digested with a 5-enzyme digestion protocol using 1 U each of SmaI, HpaII, HhaI, AciI, and BstUI (New England Biolabs, Ipswitch, MA) as described before [Miousse et al., 2015b ]. Digested DNA was then analyzed with intercalating dye (SYBR Select, ThermoFisher Scientific) by qRT-PCR on a ViiA 7 Real-Time PCR System (Applied Biosystems, Foster City, CA). Assays for determination of 5’-UTR LINE-1 and satellite DNA methylation are provided in Supplementary Table S1. Fold changes were calculated using the ΔΔCt method and normalized towards readings from a LINE-1 element ORF1 region that lacks CpG sites.
Miousse I.R., Skinner C.M., Sridharan V., Seawright J.W., Singh P., Landes R.D., Cheema A.K., Hauer-Jensen M., Boerma M, & Koturbash I. (2019). Changes in one-carbon metabolism and DNA methylation in the hearts of mice exposed to space environment-relevant doses of oxygen ions (16O). Life sciences in space research, 22, 8-15.
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3

RNA and DNA Extraction from Tumor Samples

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Fresh tumor samples preserved in RNAlater (Qiagen, Hilden, Germany) were obtained in conjunction with routine clinical sampling by a diagnostic pathologist in regional pathology departments (see 5 (link)). RNA and DNA were extracted using the Qiagen Allprep extraction kit (Qiagen) as described33 (link). DNA from whole blood was extracted by the Labmedicin Skåne Biobank.
Staaf J., Glodzik D., Bosch A., Vallon-Christersson J., Reuterswärd C., Häkkinen J., Degasperi A., Amarante T.D., Saal L.H., Hegardt C., Stobart H., Ehinger A., Larsson C., Rydén L., Loman N., Malmberg M., Kvist A., Ehrencrona H., Davies H.R., Borg Å, & Nik-Zainal S. (2019). Whole-genome-sequencing of triple negative breast cancers in a population-based clinical study. Nature medicine, 25(10), 1526-1533.
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4

Comprehensive Extraction of Biomolecules

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Tumor tissues were homogenized using the polytron PT1300 tissue homogenizer followed by additional homogenization using a Qiashredder spin column (Qiagen). RNA DNA and protein were extracted using the Allprep extraction kit (Qiagen). RNA from cells in culture was extracted using Trizol, followed by clean up with RNeasy RNA extraction kit (Qiagen).
Ostrow K.L., Donaldson K., Blakeley J., Belzberg A, & Hoke A. (2015). Immortalized Human Schwann Cell Lines Derived From Tumors of Schwannomatosis Patients. PLoS ONE, 10(12), e0144620.
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5

Transcriptomic RNA Sequencing for Differential Gene Expression

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Transcriptomic RNA sequencing was performed at the USC Norris Molecular Genomics Core. Total RNA were isolated using a Qiagen (Germatown, MD, USA) All prep Extraction kit following the manufacturer’s protocol (Qiagen Cat. No. 80284). Libraries were simultaneously prepared using an Illumina Truseq Stranded mRNA Library Preparation kit (Illumina Cat. No. 20020594; San Diego, CA, USA). Transcriptomic RNAseq libraries were sequenced on Illumina Nextseq500 at 25 million reads per sample at 2 × 75 read length. Data were trimmed normalized and analyzed using Partek Flow. Gene lists were created to detect differentially expressed genes between
Walker C., Boisvert A., Malusare P, & Culty M. (2023). Impact of Fetal Exposure to Endocrine Disrupting Chemical Mixtures on FOXA3 Gene and Protein Expression in Adult Rat Testes. International Journal of Molecular Sciences, 24(2), 1211.
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6

Targeted Sequencing for Genetic Profiling

Cited 1 time
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Samples from diagnostic and remission BM or PB underwent DNA extraction using the AllPrep Extraction Kit (Qiagen). The integrity of the samples was confirmed by visualization on a 0.8% agarose gel. All samples then underwent targeted exome capture sequencing, which included the entire coding region of KIT, as previously described.(31 (link)) Samples from relapse specimens underwent analysis of mutations of exon 8 and 17 using amplification by genomic PCR as previously described.(19 (link))
Tarlock K., Alonzo T.A., Wang Y.C., Gerbing R.B., Ries R., Loken M.R., Pardo L., Hylkema T., Joaquin J., Sarukkai L., Raimondi S.C., Hirsch B., Sung L., Aplenc R., Bernstein I., Gamis A.S., Meshinchi S, & Pollard J.A. (2019). Functional Properties of KIT Mutations are Associated with Differential Clinical Outcomes and Response to Targeted Therapeutics in CBF Acute Myeloid Leukemia. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(16), 5038-5048.
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7

RNA and DNA Extraction from Tumor Samples

Cited 2 times
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Fresh tumor samples preserved in RNAlater (Qiagen, Hilden, Germany) were obtained in conjunction with routine clinical sampling by a diagnostic pathologist in regional pathology departments (see 5 (link)). RNA and DNA were extracted using the Qiagen Allprep extraction kit (Qiagen) as described33 (link). DNA from whole blood was extracted by the Labmedicin Skåne Biobank.
Staaf J., Glodzik D., Bosch A., Vallon-Christersson J., Reuterswärd C., Häkkinen J., Degasperi A., Amarante T.D., Saal L.H., Hegardt C., Stobart H., Ehinger A., Larsson C., Rydén L., Loman N., Malmberg M., Kvist A., Ehrencrona H., Davies H.R., Borg Å, & Nik-Zainal S. (2019). Whole-genome-sequencing of triple negative breast cancers in a population-based clinical study. Nature medicine, 25(10), 1526-1533.
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8

OSCC Tissue DNA Extraction

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DNA was extracted from fresh frozen OSCC tissue (tumor) and oral cavity mucosa (matched normal). DNA extraction was performed using the Qiagen AllPrep extraction kit (Qiagen, Germantown, MD, USA), as per the manufacturer's instructions. Library preparations were processed using the TruSeq Nano preparation kit (Illumina, San Diego, CA, USA).
Satgunaseelan L., Strbenac D., Willet C., Chew T., Sadsad R., Wykes J., Low T.(., Cooper W.A., Lee C.S., Palme C.E., Yang J.Y., Clark J.R, & Gupta R. (2022). Whole genome duplication in oral squamous cell carcinoma in patients younger than 50 years: implications for prognosis and adverse clinicopathological factors. Genes, Chromosomes & Cancer, 61(9), 561-571.
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9

Progenitor Cell RNA Sequencing

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The RNA was isolated from the progenitor cells using Qiagen’s AllPrep extraction kit, per the manufacturer’s instructions. RNA-seq libraries were prepared using TruSeq™ RNA-Seq by poly(A) enrichment (Illumina) and sequenced on a HiSeq® 2000 system (Illumina) using a 50-bp single-end approach, per the manufacturer’s instructions. Sequencing statistics are included in Supplementary Table 1B.
Ciccocioppo R., Sanna P.P, & Weiss F. (2001). Cocaine-predictive stimulus induces drug-seeking behavior and neural activation in limbic brain regions after multiple months of abstinence: Reversal by D1 antagonists. Proceedings of the National Academy of Sciences of the United States of America, 98(4), 1976-1981.
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10

Quantifying HIV DNA and RNA in PBMCs

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DNA and RNA were co-extracted from 5 million freshly isolated PBMCs using the All prep extraction kit (Qiagen). Total HIV DNA (gag) and ca-RNA (gag and tat-rev) were quantified by droplet digital PCR (ddPCR) from extracted DNA or RNA, respectively, as described previously [25 ]. Copy numbers were normalized to 1 million CD4 T cells as determined by RPP30 (total cell count) and flow cytometry (percentage of CD4 T cells).
Massanella M., Yek C., Lada S.M., Nakazawa M., Shefa N., Huang K, & Richman D.D. (2018). Improved assays to measure and characterize the inducible HIV reservoir. EBioMedicine, 36, 113-121.
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