Phosphorylated rb serine 807 811

Manufactured by Cell Signaling Technology

Sourced in United States

Phosphorylated Rb (Serine 807/811) is a laboratory reagent used to detect the phosphorylation of the retinoblastoma (Rb) protein at serine residues 807 and 811. This phosphorylation event is a crucial regulatory mechanism that controls the activity of the Rb protein, which plays a central role in cell cycle progression and proliferation.

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Lab products found in correlation

Ab133534, by Abcam (1 mentions) Antibodies against cleaved caspase 3, by Cell Signaling Technology (1 mentions) γh2ax, by Cell Signaling Technology (1 mentions) Vinculin, by Merck Group (1 mentions) Vinculin, by LI COR (1 mentions) Image studio software, by LI COR (1 mentions)

Close spelling variants of this product

Phosphorylated Rb (6 citations)

2 protocols using phosphorylated rb serine 807 811

Immunohistochemical Analysis of Tumor Samples

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Tumors were fixed in 10% buffered formalin overnight before paraffin embedding. Paraffin blocks were sectioned and stained with haematoxylin and eosin (H&E). The value for tumor cell density was calculated as the number of tumor cells per mm². For immunohistochemical staining analysis, antibodies against Cleaved-Caspase-3 (#9661, RRID: AB_2341188), γH2AX (#2577, RRID: AB_2118010), and phosphorylated Rb (Serine 807/811) (#8516, RRID: AB_11178658) were from Cell Signaling Technology (USA). Antibodies against Ki67 (#ab15580, RRID: AB_443209) and RAD51 (#ab133534, RRID: AB_2722613) were from Abcam (UK). Antibodies against MYC (#10828-1-AP, RRID: AB_2148585) was from Proteintech (USA). For each tumor sample, 3–5 random 40 × fields were scored. Digital images were submitted for quantitative image analysis using Image Pro-plus software.

Yi J., Liu C., Tao Z., Wang M., Jia Y., Sang X., Shen L., Xue Y., Jiang K., Luo F., Liu P, & Cheng H. (2019). MYC status as a determinant of synergistic response to Olaparib and Palbociclib in ovarian cancer. EBioMedicine, 43, 225-237.

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Western Blot Analysis of Apoptosis and Cell Cycle Markers

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Cells were harvested in RIPA lysis buffer containing a proteinase cocktail (Thermo Scientific, USA). Cell lysates were then analyzed by western blot. Antibodies against Cleaved-PARP (#5625, RRID: AB_10699459), MYC (#5605, RRID: AB_1903938) and phosphorylated Rb (Serine 807/811) (#8516, RRID: AB_11178658) were from Cell Signaling Technology (USA). Vinculin (#V9131, RRID: AB_477629) was from Sigma-Aldrich (USA). Immunofluorescently labeled secondary antibodies to rabbit-IgG (Molecular Probes, USA) or mouse-IgG (Rockland Immunochemicals, USA) were used. Western blots were imaged with Odyssey (LI-COR Biosciences, USA). MYC protein abundance was quantified using Image Studio software (LI-COR Biosciences) and normalized to Vinculin.

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