Losartan

Manufactured by Selleck Chemicals

Sourced in United States

Losartan is a laboratory reagent used in research and development applications. It functions as an angiotensin II receptor antagonist, which is a class of compounds that block the action of the hormone angiotensin II. This activity is useful for various biological and pharmacological studies.

Automatically generated - may contain errors

Lab products found in correlation

Captopril, by Selleck Chemicals (2 mentions) U73122, by Selleck Chemicals (2 mentions) Ang 2, by Merck Group (2 mentions) Ganciclovir, by Selleck Chemicals (1 mentions) Rtu vectastain universal elite abc kit, by Vector Laboratories (1 mentions) Vectafluor r t u dylight 488 anti mouse, by Vector Laboratories (1 mentions) Vectashield, by Thermo Fisher Scientific (1 mentions) Recombinant human tgf β1, by R&D Systems (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Celltiter 96 aqueous one solution cell proliferation assay, by Promega (1 mentions) Wortmannin, by Selleck Chemicals (1 mentions) Mk 2206, by Selleck Chemicals (1 mentions) Ng nitro l arginine methyl ester l name, by Selleck Chemicals (1 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions) Indomethacin, by Merck Group (1 mentions) Phenylephrine, by Merck Group (1 mentions) Sodium nitroprusside, by Merck Group (1 mentions) Hydroxyproline detection kit, by Nanjing Jiancheng (1 mentions) Pentobarbital sodium, by Merck Group (1 mentions) Simvastatin, by Merck Group (1 mentions)

12 protocols using losartan

Brain Tumor Implantation and Treatment

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Brain tumor implantation in the forebrain (50,000 to 100,000 cells), cranial window surgery, and tumor resection as part of the SOC treatment regimen were conducted as previously described (17 (link), 52 (link), 53 (link)). Mice were allowed to recover for 10 to 14 d after cranial window surgery prior to tumor implantation and for 2 d after resection surgery (as part of the SOC or bihemispheric model) prior to treatment initiation.
When tumors reached 1 mm in diameter (7 to 10 d post-implantation), mice were treated daily with phosphate-buffered saline (PBS;control) or losartan (Selleckchem) daily at 60 mg/kg until study endpoint. After 1 wk of losartan pretreatment, mice were treated with IgG (control) or anti-PD1 (BioxCell, RMP1-14) every 3 d for three doses at 200 μg/mouse. SOC mice received concurrently with losartan: 5 d of consecutive radiotherapy (2 Gy/d) and 10 d of consecutive chemotherapy (temozolomide, Selleckchem) at 25 mg/kg. All drugs were injected i.p. For flow cytometry, scRNASeq, intravital imaging, histology, and edema measurements, mice were imaged/sacrificed after the third dose of anti-PD1 and/or 2 wk of losartan treatment.

Datta M., Chatterjee S., Perez E.M., Gritsch S., Roberge S., Duquette M., Chen I.X., Naxerova K., Kumar A.S., Ghosh M., Emblem K.E., Ng M.R., Ho W.W., Kumar P., Krishnan S., Dong X., Speranza M.C., Neagu M.R., Iorgulescu J.B., Huang R.Y., Youssef G., Reardon D.A., Sharpe A.H., Freeman G.J., Suvà M.L., Xu L, & Jain R.K. (2023). Losartan controls immune checkpoint blocker-induced edema and improves survival in glioblastoma mouse models. Proceedings of the National Academy of Sciences of the United States of America, 120(6), e2219199120.

+ Open protocol

+ Expand

TGF-β1 Signaling Pathway Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following reagents were obtained from the respective commercial vendors. Recombinant human TGF-β1 from R&D Systems (Minneapolis, MN, USA), losartan from Selleck Chemicals (Houston, TX, USA), dexamethasone from Sigma-Aldrich (St. Louis, MO, USA), ganciclovir from Selleck Chemicals, normal horse serum (RTU Vectastain Universal Elite ABC Kit) from Vector Laboratories (Burlingame, CA, USA), murine monoclonal anti-CMV IE antibody (anti-CMV Immediate Early Antigen Antibody, #LS-C103255) from LSBio (Seattle, WA, USA), a goat anti-mouse antibody (VectaFluor R.T.U. DyLight 488 anti-mouse) from Vector Laboratories, rhodamine phalloidin and Vecta-Stain mounting media (VECTASHIELD) from Invitrogen (Carlsbad, CA, USA), a TGF-β1 enzyme-linked immunosorbent assay (ELISA) kit (Total TGF-β1 ELISA Kit with precoated plates) from BioLegend (San Diego, CA, USA), and a MTS assay kit (CellTiter 96^® AQueous One Solution Cell Proliferation Assay) from Promega (Medison, WI, USA).

Choi J.A., Kim J.E., Ju H.H., Lee J., Jee D., Park C.K, & Paik S.Y. (2019). The effects of losartan on cytomegalovirus infection in human trabecular meshwork cells. PLoS ONE, 14(6), e0218471.

+ Open protocol

+ Expand

Angiotensin II Signaling Inhibition in NK/T-cell Lymphoma

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human NK/T-cell lymphoma cell lines SNK-6 (ATCC) were cultured in RPMI-1640 (BioChannel Biological Technology Co., Ltd.) supplemented with 10% fetal bovine serum (FBS; BioChannel Biological Technology Co., Ltd.) and 700 U/ml interleukin-2 (IL-2), and incubated at 37°C and 5% CO₂. Losartan (an antagonist of AT1R [18 (link)]; 10⁻⁵ M), wortmannin (an inhibitor of PI3K [19 (link)]; 10⁻⁷ nM, Selleck, Shanghai, China) or MK2206 (an inhibitor of Akt [20 (link)]; 5 × 10⁻⁶ μM, Selleck) pretreatment lasted for 30 min, then, Ang II (10⁻⁶ M) was added.

Zhang G.H., Miao F.A., Xu J.G, & Zhang Y. (2020). Angiotensin II enhances the proliferation of Natural Killer/T-cell lymphoma cells via activating PI3K/Akt signaling pathway. Bioscience Reports, 40(10), BSR20202388.

+ Open protocol

+ Expand

Vascular Tension Modulation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Phenylephrine (PE; Sigma-Aldrich; Merck KGaA), sodium nitroprusside (SNP; Sigma-Aldrich; Merck KGaA), indomethacin (Cyclooxygenase 2 inhibitor; Sigma-Aldrich; Merck KGaA), EDTA (Sigma-Aldrich; Merck KGaA), dimethyl sulfoxide (DMSO; Sigma-Aldrich; Merck KGaA), acetylcholine (Ach; Apexbio Corporation), MDL12330A (AC-V inhibitor, Apexbio Corporation). NPS2143 (CaSR inhibitor, R&D Systems, Inc.), NPSR568 (CaSR agonist, R&D Systems, Inc.). N^G-nitro-L-arginine methyl ester (L-NAME, NOS inhibitor; Selleck Chemicals), U73122 (PLC inhibitor, Selleck Chemicals), 2-aminoethoxydiphenyl borate (2-APB, IP₃ inhibitor; Selleck Chemicals), Captopril (CAP, angiotensin-converting enzyme inhibitor, Selleck Chemicals), Losartan [LOS, angiotensin II receptor type 1 (AT1R) inhibitor, Selleck Chemicals]. All of the other chemicals were of reagent grade. indomethacin, L-NAME, NPS2143, NPSR568, U73122, 2-APB, MDL12330A, CAP and LOS were dissolved in DMSO to prepare stock solutions (10⁻¹ mol/l), with the use of double distilled water prepared into the corresponding working fluid. The other agents were prepared in double distilled water. Studies have shown no notable effects on the tension development of isolated arteries with concentrations of DMSO<0.2% (27 (link)–29 (link)).

Zhang W., Sun R., Zhong H., Tang N., Liu Y., Zhao Y., Zhang T, & He F. (2019). CaSR participates in the regulation of vascular tension in the mesentery of hypertensive rats via the PLC-IP3/AC-V/cAMP/RAS pathway. Molecular Medicine Reports, 20(5), 4433-4448.

+ Open protocol

+ Expand

Antifibrotic Pathway Modulation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Angiotensin II, Simvastatin, and Pentobarbital sodium were each purchased from Sigma (Sigma, USA). Losartan and Y-27632 were obtained from Selleck (Selleck Chemicals, USA). TGF-B1 was purchased from PROSPEC (PROSPEC, Israel). The ROCK-1, CTGF, Collagen–I (α1), TGF-β1, and Smad 2/3 antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, Delaware Avenue, CA). The RhoA pull-down was provided by Cytoskeleton (Cytoskeleton, USA). All DNA probes were synthesized by Invitrogen (Invitrogen, USA). The HE and Masson trichrome stains were both purchased from Maxin (Fuzhou, Fujian, China). The hydroxyproline detection kits were purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, Ghina). Fetal calf serum and Dulbecco's modified Eagle medium (DMEM) were obtained from Gibco BRL (Gaithersburg, MD). All buffer solutions used in this study were prepared in our lab. Additional commonly used chemicals and materials were purchased from standard commercial sources.

Liu L.J., Yao F.J., Lu G.H., Xu C.G., Xu Z., Tang K., Cheng Y.J., Gao X.R, & Wu S.H. (2016). The Role of the Rho/ROCK Pathway in Ang II and TGF-β1-Induced Atrial Remodeling. PLoS ONE, 11(9), e0161625.

+ Open protocol

+ Expand

Angiotensin II Signaling Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

Angiotensin II (Ang II), diphenyleneiodonium (DPI) and catalase (CAT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). AVE 0991 was purchased from Apexbio (Houston, TX, USA). Losartan was purchased from Selleck Chemicals (Houston, TX, USA). Small interfering RNA (siRNA) targeting NOX4 was purchased from GenePharma (Shanghai, China).

Zhang M., Zhu X., Tong H., Lou A., Li Y., Li Y., Su L, & Li X. (2019). AVE 0991 Attenuates Pyroptosis and Liver Damage after Heatstroke by Inhibiting the ROS-NLRP3 Inflammatory Signalling Pathway. BioMed Research International, 2019, 1806234.

+ Open protocol

+ Expand

Cardiac Myofibroblast Differentiation on Hydrogels

Check if the same lab product or an alternative is used in the 5 most similar protocols

To determine the effect of substrate stiffness and pore size on cardiac myofibroblast differentiation, cardiac fibroblasts were seeded at a concentration of 2 × 10⁵ cells/cm² on collagen-coated PA hydrogels with different ratios of acrylamide (%)/MBA (%). Cardiac fibroblasts cultured on a cell culture plate (with a stiffness of ~1 GPa) were used as a positive control. Following the 5-day culture on PA hydrogels, these cells were subjected to evaluation of cardiac myofibroblast differentiation through α-SMA immunofluorescence staining and gene (α-SMA, TGF-β1, Col I, Col III, AT₁R and Smad7) expression analysis (described later). Further, the concentration of angiotensin II in conditioned medium of cardiac fibroblasts cultured on 30 kPa substrates and cell culture plate was determined by ELISA (described later).
In addition, to explore the role of AT₁R in cardiac myofibroblast differentiation, losartan (AT₁R inhibitor) (Selleckchem, Houston, USA) at 10⁻⁷ M was given to cardiac fibroblasts cultured on PA hydrogels on day 4. The treatment was lasted for 1 day. The cells were then subjected to cardiac myofibroblast differentiation assessment.

Yong K.W., Li Y., Liu F., Bin G.a.o., Lu T.J., Wan Abas W.A., Wan Safwani W.K., Pingguan-Murphy B., Ma Y., Xu F, & Huang G. (2016). Paracrine Effects of Adipose-Derived Stem Cells on Matrix Stiffness-Induced Cardiac Myofibroblast Differentiation via Angiotensin II Type 1 Receptor and Smad7. Scientific Reports, 6, 33067.

+ Open protocol

+ Expand

Neonatal Rat Cardiomyocyte and Fibroblast Isolation

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary neonatal rat cardiomyocytes (NRCMs) and cardiac fibroblasts (NRCFs) were obtained from 1 to 3-day-old newborn SD rats (Airforce Medical University, Xi’an, China). Briefly, 30 newborn rats were euthanasia by cervical dislocation after anesthetized with 2% isoflurane for 2 min. The isolated left ventricle of heart was digested in PBS containing collagenase type II (Worthington, NJ, USA) and pancreatin (Sigma, MO, USA), then the cells were collected and cultured in Dulbecco modified Eagle medium (DMEM) (Gibco, Invitrogen Inc.) supplemented with 2 mM glutamine, 10% heat-inactivated fetal bovine serum and antibiotics (100 U/ml of penicillin A and 100 U/ml of streptomycin) at 37 ℃ in a humidified incubator containing 5% CO₂ for 2–4 h to separate fibroblasts and cardiomyocytes, and then treated with adenovirus-TOR2A shRNA (GeneChem Co., Shanghai, China), Ang II (10^–6 M; Sigma) [28 (link), 30 (link)] or losartan (10^–6 M; Selleck, Shanghai, China) for 24 h.

Dang J.Y., Zhang W., Chu Y., Chen J.H., Ji Z.L, & Feng P. (2024). Downregulation of salusins alleviates hypertrophic cardiomyopathy via attenuating oxidative stress and autophagy. European Journal of Medical Research, 29, 109.

+ Open protocol

+ Expand

Alcohol-Induced Cardiac Injury Mitigation

Check if the same lab product or an alternative is used in the 5 most similar protocols

For treating hiPSC-CMs, 100-mM alcohol was diluted in B27, and cells were treated for different durations. Then, 1 μM losartan (Selleck, USA) was diluted in B27, and hiPSC-CMs were co-treated with alcohol.

Song Y., Li H., Ma S., Zhu M., Lu W.J., Lan F, & Cui M. (2022). Losartan protects human stem cell-derived cardiomyocytes from angiotensin II-induced alcoholic cardiotoxicity. Cell Death Discovery, 8, 134.

+ Open protocol

+ Expand

Chemical Compound Procurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

NPS2143 was purchased from Tocris Bioscience Co., Ltd. U73122, captopril and losartan were obtained from Selleck Chemicals. 2-APB was purchased from Abcam and MDL12330A was obtained from ApexBio Technology LLC.

Zhao Y., Tang N., Xi D., Huang Z., Zhang T., Liu Y., Wang L., Tang Y., Zhong H, & He F. (2020). Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin-angiotensin system. Experimental and Therapeutic Medicine, 20(2), 818-829.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!