Elecsys vitamin d3 assay

Sociodemographic characteristics, including age at marriage, educational level, occupation, monthly income, sun exposure, and parity was collected using a questionnaire. Dietary data were also collected, and included information regarding the consumption of foods containing vitamin D, such as fish, yogurt, and cheese. Details such as types and amounts of food, preparation methods, and serving size of each item were collected by asking the participants to recall what they had consumed within a 3-day period (24-hour dietary recall) retrospective. Daily intake of vitamin D for each participant was estimated by using Food Processor for Windows (version 7.71; ESHA Research, Inc., Salem, OR, USA). A daily vitamin D intake of a minimum of 600 IU (15.0 µg) was considered adequate based on the dietary reference intakes for women.22
To measure plasma vitamin D levels, blood samples were collected after a 12-hours fasting period. The samples were centrifuged at 2,000 rpm for 20 minutes to separate the plasma, which was then stored at -70°C for later analysis. Roche Elecsys vitamin D3 assay (Roche Diagnostics GmbH, Mannheim, Germany), using a Cobas e601 immunoassay analyzer was used to estimate plasma vitamin D3 levels. Measured vitamin D levels were then classified as sufficient (>75.0 nmol/L), insufficient (50.0-75.0 nmol/L), or deficient (<50.0 nmol/L).23 (link)