Cxcr3ko

The experimental procedures and use of animals were performed in accordance with the Association of Research for Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research, and all protocols were approved by the Institutional Animal Care and Use Committee at the University of Texas Medical Branch. C57BL/6 J, CXCR3KO and GFP transgenic mice were obtained from Jackson Laboratory (Bar Harbor, ME, USA) and maintained on a 12:12 light/dark cycle with food and water available ad libitum.

WT C57BL/6 and BALB/c mice were purchased from Charles River Laboratories. Rag1^KO, Ifng^KO, Ifnar1^KO, Klrk1^KO, Sting^KO, Batf3^KO, Ticam1^KO, Myd88^KO, Ccr5^KO, Cxcr3^KO, CD11c-DTR, and ROSA^DTR C57BL/6 mice were purchased from the Jackson Laboratory. Single-KO mice were bred to generate double-KO mice, including Ticam1^KO Myd88^KO, Ccr5^KO Cxcr3^KO, and Ifnar1^KO Cxcr3^KO. Ncr1^iCre mice (a gift from Eric Vivier, Aix Marseille University Hospital, Marseille, France) were bred with ROSA^DTR mice to generate Ncr1^iCre ROSA^DTR mice. MMTV-PyMt^tg mice (a gift from David DeNardo, Washington University, St. Louis, Missouri, USA) were maintained on BALB/c background. All mice were housed in pathogen-free facilities in accordance with the guidelines instituted by the animal study committees of Massachusetts General Hospital.

C57BL/6J WT, Rag1-KO, Cxcr3-KO, Cxcl10-KO, Tgfa-KO, and Il10-KO mice were purchased form The Jackson Laboratory. Mice were housed in a temperature- and humidity-controlled and specific pathogen–free animal facility with a 12-hour light-dark cycle at the University of Pittsburgh. Food and water were available ad libitum. As detailed below, we followed STAIR guidelines in the design of animal experiments, including testing aged mice of both sexes, use of 2 different stroke models, evaluation of long-term functional stroke outcomes, monitoring of regional CBF, etc. Mice were randomly assigned to experimental groups and received randomized treatments using a lottery-drawing box. All efforts were made to minimize animal suffering and the number of animals used. All surgeries, treatments, and data analyses were performed by investigators blinded to animal genotypes and experimental grouping wherever feasible.