Bifidobacterium longum subsp. infantis ATCC 15697 and B. breve SC95 were routinely grown for 48 h anaerobically at 37 °C in the semisynthetic de Man-Rogosa-Sharpe (MRS) broth or MRS agar (Becton Dickinson) supplemented with 1 % (wt/vol) L-cysteine. Single colony isolates were inoculated into modified MRS (mMRS) without sugar supplemented with 2 % of filter-sterilized (wt/vol) lactose (LAC) (Sigma Aldrich), 2 % glucose (GLU) (Fisher) or 2 % purified human milk oligosaccharide mixture (HMO) as the sole carbon source. HMO was kindly provided by the laboratory of Dr. Bruce German (Department of Food Science and Technology, University of California, Davis) and purified according to the method described in Gnoth et al. [53 (link)].
Lactose lac
Manufactured by Merck Group
Sourced in United States
Lactose (LAC) is a disaccharide sugar found in milk and dairy products. It is a key component in various laboratory applications, serving as a substrate for enzymatic reactions and microbial growth. Lactose provides a reliable source of carbohydrates for culturing and maintaining a wide range of microorganisms in controlled laboratory environments.
Automatically generated - may contain errors
Lab products found in correlation
Mrs agar, by BD (1 mentions)
Glucose glu, by Thermo Fisher Scientific (1 mentions)
Methanol, by Chron Chemicals (1 mentions)
Acetonitrile, by Chron Chemicals (1 mentions)
Ellagic acid, by Yuanye Bio-Technology (1 mentions)
Phloridzin, by Yuanye Bio-Technology (1 mentions)
Isoquercitrin, by Yuanye Bio-Technology (1 mentions)
Hyperin, by Yuanye Bio-Technology (1 mentions)
Fucose fuc, by Merck Group (1 mentions)
Quercetin, by Yuanye Bio-Technology (1 mentions)
Quercitrin, by Yuanye Bio-Technology (1 mentions)
1 phenyl 3 methyl 5 pyrazolone pmp, by Adamas (1 mentions)
2 protocols using lactose lac
1
Bifidobacterium Growth on Lactose, Glucose, and HMO
2
Characterization of Apple Peel Polysaccharides
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Dried apple peel, as a by-product of apple chips, was obtained from Meiqingyuan Food Co. (Sichuan, China). A. oryzae (CICC 2339) in lyophilized powder form was acquired from China Center of Industrial Culture Collection (CICC) and it was kept at 4 °C until use. The standards of mannose (Man), rhamnose (Rha), galacturonic acid (GalA), glucose (Glc), galactose (Gal), xylose (Xyl), arabinose (Ara) and fucose (Fuc) and lactose (Lac) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). High-performance liquid chromatograph (HPLC)-grade chlorogenic acid, hyperin, isoquercitrin, ellagic acid, phloridzin, quercitrin, quercetin and Folin-Ciocalteu’s reagent was obtained from YuanyeBio-Technology Co., Ltd (Shanghai, China). 1-Phenyl-3-methyl-5-pyrazolone (PMP) was obtained from Adamas Reagent Co., Ltd. (Shanghai, China). Acetonitrile and methanol of chromatography grade and other chemicals of analytical grade were purchased from Kelong Chemical Reagent Co., Ltd (Chengdu, China).
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