Ca9-22 (derived from OSCC of the gingiva) and HSC-3 (derived from OSCC of the tongue, referred to as HSC3 hereafter) cell lines were obtained from the RIKEN Bioresource Center (Tsukuba, Japan). HSC5 (derived from SCC of the skin) and Ho-1-N-1 (derived from OSCC of the buccal mucosa, referred to as HO1N1 hereafter) were obtained from the Japanese Collection of Research Bioresources (Osaka, Japan). BHY (derived from OSCC of the gingiva) [12 (link)] was provided by Dr. Masato Okamoto (Tsurumi University). SAS (derived from OSCC of the tongue) [13 (link)] and HSC-4 (derived from OSCC of the tongue, referred to as HSC4 hereafter) [14 (link)] were provided by Dr. Masao Saito (Yamanashi University). 293A was purchased from Thermo Fisher Scientific Co. These cells were maintained in Dulbecco’s modified Eagle’s medium/nutrient mixture F-12 (Sigma-Aldrich) with 10% fetal bovine serum at 37°C in the presence of 5% CO2. Primary human keratinocytes isolated from neonatal foreskins were obtained from Kurabo Co. (Osaka, Japan) and were maintained in HuMedia-KG2 (Kurabo) at 37°C in the presence of 5% CO2.
Hsc 3
Manufactured by RIKEN BioResource Center
Sourced in Japan
The HSC-3 is a laboratory equipment designed for handling and analyzing cell samples. It provides a controlled environment for cell culture and related applications. The core function of the HSC-3 is to maintain optimal conditions for cell growth and experimentation.
Automatically generated - may contain errors
Lab products found in correlation
Hsc 4, by RIKEN BioResource Center (14 mentions)
Ca9 22, by RIKEN BioResource Center (12 mentions)
Hsc 2, by RIKEN BioResource Center (8 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Cal 27, by RIKEN BioResource Center (4 mentions)
Penicillin, by Thermo Fisher Scientific (4 mentions)
Streptomycin, by Thermo Fisher Scientific (4 mentions)
Oecm1, by RIKEN BioResource Center (3 mentions)
Ho 1 u 1, by RIKEN BioResource Center (3 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (3 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (2 mentions)
Calu 1, by American Type Culture Collection (2 mentions)
Jurkat, by American Type Culture Collection (2 mentions)
Penicillin, by Sartorius (2 mentions)
Streptomycin, by Sartorius (2 mentions)
Amphotericin b, by Sartorius (2 mentions)
Dulbecco modified eagle medium (dmem), by Nissui Pharmaceutical (2 mentions)
Hsc 3 m3, by RIKEN BioResource Center (2 mentions)
Ho 1 n 1, by RIKEN BioResource Center (2 mentions)
31 protocols using hsc 3
1
Oral Squamous Cell Carcinoma Cell Lines
2
Culturing OSCC Cell Lines
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OSCC cell lines (HSC2, HSC3, HSC4, SAS and Ca9-22) and HaCaT were obtained from the Cell Bank (RIKEN BioResource Center, Tsukuba, Japan). Cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich; Merck KGaA) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 100 µg/ml streptomycin and 100 U/ml penicillin (Thermo Fisher scientific) at 37°C in a humidified atmosphere containing 5% CO2.
3
Oral Squamous Cell Carcinoma Cell Lines
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Human cell lines derived from OSCC HSC-3 and HSC-4 cells were obtained from Riken BRC Cell Bank (Tsukuba, Japan). To analyze the association between SOX9 expression and metastasis, two cell lines, namely HSC-3, a metastatic cell line that was established from the metastatic lymph node of a 63-year-old man with poorly differentiated SCC and HSC-4, a non-metastatic cell line that was established from a metastatic lymph node in a 63-year-old man with well-differentiated SCC, were selected (21 (link)). The cells were maintained in Minimum Essential Medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal calf serum (FCS) under a humidified atmosphere with 5% CO2 and 95% air at 37°C.
4
Establishment and Characterization of HNSCC Cell Lines
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HNSCC cell lines HSC-3, HSC-4 (tongue SCC, DR1/4) and Sa-3 (gingival SCC, DR9/10) were provided by the RIKEN Bio-Resource Center (Tsukuba, Japan). CA9-22 (gingival SCC) and HPC-92Y (hypopharyngeal SCC) were kindly provided by Dr. Yasuharu Nishimura (Dep. of Immunogenetics, Kumamoto University, Kumamoto, Japan) and Dr. Syunsuke Yanoma (Yokohama Tsurugamine Hospital, Yokohama, Japan), respectively. SAS (tongue SCC), Calu-1 (non-small cell lung carcinoma) and 5637 (bladder cancer) were purchased from American Type Culture Collection (Manassas, VA). All cell lines were maintained in RPMI 1640 (nacalai tesque, Kyoto, Japan) supplemented with 10% fetal bovine serum.
5
Establishing HLA-DR Expressing Cell Lines
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Mouse fibroblast cell lines that were transfected with plasmids expressing individual human HLA-DR molecules (L-DR4, −9 or −53) were kindly provided by Dr. Robert W Karr (Karr Pharma, Saint Louis, MO) and by Dr. Takehiko Sasazuki (Kyushu University, Fukuoka, Japan). The cell lines HSC3 (tongue squamous cell carcinoma SCC, DR15/15), HSC4 (tongue SCC, DR1/4, −53), Sa-3 (gingival SCC, DR9/10, −53) and Lu65 (lung large cell carcinoma, DR4/15, −53) were supplied by the RIKEN Bio-Resource Center (Tsukuba, Japan). The HNSCC cell line HPC92Y (hypopharyngeal SCC, DR4/9, −53) was kindly provided by Dr. Syunsuke Yanoma (Yokohama Tsurugamine Hospital, Yokohama, Japan). Tumor cell lines SAS (tongue SCC, DR9/15, −53), Calu-1 (Lung SCC, DR7/14, −53), WiDr (colon adenocarcinoma, DR4/7, −53) and Jurkat (T cell lymphoma, a cell line not expressing HLA-DR) were purchased from the American Type Culture Collection (Manassas, VA, USA). All cell lines were maintained in a tissue culture medium as recommended by the supplier.
6
Cultivation of Human Oral Cancer Cell Lines
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The human oral squamous cell carcinoma (OSCC) cell lines SAS (#RCB1974), HSC-3 (#RCB1975), HSC-4 (#RCB1902), and Ca9-22 (#RCB1976) were purchased from RIKEN BRC Cell Bank (Tsukuba, Japan; 2013), where the cell lines were authenticated by STR profiling before distribution. Cells were cultured in Minimum Essential Medium (MEM, Invitrogen/GIBCO, Carlsbad, CA) supplemented with 10% fetal bovine serum (Nichirei Bio., Tokyo, Japan) in a humidified atmosphere containing 5% CO2 at 37°C according to supplier’s instructions. Cells were used within 6 months of resuscitation.
7
Cultivation of Human and Mouse OSCC Cell Lines
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Human OSCC cell lines HSC-2, HSC-3, and Ca 9-22 were obtained from the RIKEN Bio Resource Center Cell Bank. These cells were cultured in RPMI 1640 (Thermo Fisher Scientific, Waltham, MA, USA) medium supplemented with 10% fetal bovine serum (FBS; GIBCO, NY, USA) containing 1% penicillin–streptomycin (Sigma-Aldrich, St Louis, MO, USA) and 1% sodium pyruvate (Wako, Osaka, Japan) in a wet carbon dioxide (CO2) gas incubator set at 5% CO2.
The mouse OSCC cell line NR-S1K was derived from the NR-S1 cell line, which in turn was established from C3H/HeN mice [14 (link)]. Cells were maintained in RPMI1640 medium supplemented with 10% FBS.
The mouse OSCC cell line NR-S1K was derived from the NR-S1 cell line, which in turn was established from C3H/HeN mice [14 (link)]. Cells were maintained in RPMI1640 medium supplemented with 10% FBS.
8
Gingival Epithelial Cell Culture
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Reagents. OCT (maxacalcitol) and 1α,25-dihydroxy vitamin D 3 (calcitriol) were provided by Chugai Pharmaceutical Corporation (Tokyo, Japan). 1α,25dihydroxy vitamin D 2 (ercalcitriol), an active metabolite of vitamin D 2 , was obtained from R&D Systems (Minneapolis, MN, USA). Other reagents were obtained from Sigma-Aldrich unless otherwise indicated.
Human gingival/oral epithelial cell lines. The human gingival epithelial cell line Ca9-22 established from squamous cell carcinoma was obtained from the Japanese Collection of Research Biosources Cell Bank (Hokkaido, Japan). The human oral epithelial cell lines HSC-2, HSC-3, and HSC-4 established from squamous cell carcinoma were obtained from RIKEN BioResource Center (Ibaraki, Japan). Ca9-22, HSC-2, HSC-3, and HSC-4 cells were grown in E-MEM (Nacalai Tesque, Kyoto, Japan) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (HyClone, Logan, UT, USA) and 1% antibiotic-antimycotic mixture. Cells were incubated in medium containing 5% FBS unless otherwise indicated.
Human gingival/oral epithelial cell lines. The human gingival epithelial cell line Ca9-22 established from squamous cell carcinoma was obtained from the Japanese Collection of Research Biosources Cell Bank (Hokkaido, Japan). The human oral epithelial cell lines HSC-2, HSC-3, and HSC-4 established from squamous cell carcinoma were obtained from RIKEN BioResource Center (Ibaraki, Japan). Ca9-22, HSC-2, HSC-3, and HSC-4 cells were grown in E-MEM (Nacalai Tesque, Kyoto, Japan) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (HyClone, Logan, UT, USA) and 1% antibiotic-antimycotic mixture. Cells were incubated in medium containing 5% FBS unless otherwise indicated.
9
Culturing Human Oral Cancer Cell Lines
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Human oral squamous cell carcinoma cell lines HSC-3, Ca9-22, and OECM-1 were obtained from the Bioresource Collection and Research Center, Hsinchu, Taiwan (https://www.bcrc.firdi.org.tw ). HSC-3 and Ca9-22 cells were cultured in DMEM/F12 medium (Thermo Fisher Scientific, Waltham, MA, USA), and OECM-1 cells were cultured in RPMI 1640 medium (Thermo Fisher Scientific, Waltham, MA, USA). All cell culture media were supplemented with 10% fetal bovine serum (FBS; Biological Industries, Beit Haemek, Israel) and 1% penicillin–streptomycin-amphotericin B (Thermo Fisher Scientific, Waltham, MA, USA). Cells were maintained at 37 °C in a humidified incubator with 5% CO2 incubator.
10
Culturing Human Oral SCC Cell Lines
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The human oral SCC cell line SAS and HSC-3 cells were obtained from the RIKEN BRC CELL BANK (Tsukuba, Japan), and Ca9-22 cells were from the Japanese Collection of Research Bioresources (Tokyo, Japan). Cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 5% fetal bovine serum, 4 mM l -glutamine, 100 μg/ml penicillin and 100 μg/ml streptomycin, and then grown in an incubator at 37 °C in a humidified atmosphere with 5% CO2.
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